蛋白的

PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

And Homo Sapien fetal brain cDNA Library was used to screen PRL-3-interacting proteins. 3. Identification and bioinformatical analysis of CDH22, a candidate PRL-3-interacting protein Interaction between PRL-3 and CDH22, a candidate PRL-3- interacting protein was identified by GST-pull down assay, co-immunoprecipitation assay and co-localization analysis in vivo and in vitro.

PRL-3相互作用蛋白的鉴定及其功能的生物信息学分析运用基于蛋白质水平的谷胱甘肽-S-转移酶融合蛋白沉淀、免疫共沉淀和共定位分析等技术对酵母双杂交系统初步筛选的结果进行体内和体外的验证。

We will look at two studies that address the 3-dimensional conformation of the native prion protein and the infectious scrapie state using cryoEM and X-ray crystallography.

我们将探讨两个分别使用低温电子显微镜和X射线结晶法阐明了自然状态下的朊蛋白和传染性的瘙痒病状态下的朊蛋白的三维构象的研究。

The expressed apoE protein in central neuronal system functions potentially in deposition and clearance of amyloidal peptide, sustaining the stabilization of microtubule proteins, cellular signal transduction, immune regulation, glucose metabolism and oxidation stress.

中枢神经系统中的apoE对脑组织淀粉样蛋白的沉积与清除、微管蛋白稳定性的维持、细胞信号转导、免疫调节、葡萄糖代谢、氧化应激等都有潜在的作用，apoE在体内发挥十分重要的生物学活性。

DPPⅢ may be involved in the alteration of crystallin during the development of lenses, and it may play an important role in the formation and aggravation of age-related cataract.

晶状体中DPPⅢ随年龄的增长表达发生变化，可能与晶状体发育中一些晶状体蛋白的变化有关，推测DPPⅢ可能在年龄相关性白内障的形成过程中参与晶状体蛋白的降解，促进白内障的发展。

In the preparation of the material, VitC was added, and the protective effect of antioxydant was tested by investigating the changes of the crystallin during the cataractogenesis which included the content, component of polypeptide and the conformation of the secondary structure of the protein.

结果在VitC的保护作用下晶状体蛋白的低分子量蛋白的含量明显增加，PAGE电泳中出现多条分子量较低的蛋白条带，但SDS-PAGE的泳带无明显改变，CD谱显示α-螺旋增加和β-折叠减少。

The preparation of the soya cell protein with the material of the low-denatured soybean powder of the degrease, study on the influence of quality of material on the production technology and the quality of the production.

以低变性脱脂豆粉为原料生产大豆组织蛋白，详细讨论了原料的质量对生产工艺和产品质量的影响，指出了生产高质量的大豆组织蛋白的较适宜的工艺条件。

According to the condition of the dispase and alkaline proteinase, we combine the two enzymes so as to get the most optimization method to improve the yield of dissolvable peptide.

通过对单酶水解条件的摸索，将选择的两种酶以适当的比例混合，并且通过控制反应条件，提高蛋清蛋白的水解度和可溶性肽的得率，从而研制出一种适合蛋清白蛋白水解的复合酶。

Objective GFP-streptavidin bi-functional fusion protein was generated and characterized in order to demonstrate our novel platform for efficiently and durably modifying the cell surface with SA-tagged bi-functional proteins.

目的 GFP-SA双功能融合蛋白的制备及其鉴定研究，以展示我们建立的技术平台，即用含链亲和素的双功能融合蛋白对生物素化的细胞表面进行高效的锚定修饰。

The embryonic analysis results indicated that the disruption of oxt gene would lead to the disruption of wg transcription and the expression of engrailed gene, as the wg transcription is dependant on the Engrailed.

进一步结合运用FLP-FRT系统，用缺损GFP蛋白的表达来标记纯合的oxt突变基因克隆，针对眼睛成虫盘的克隆分析结果表明，在oxt突变克隆中，Hh下游的Ci蛋白的表达区域完全消失。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。