蛋白的

The way of preparing mouse Nanog fusion protein from the inclusion body was established, which was prepared for activation detection and mechanism of gene expression and regulation.

建立了一种可行的从包涵体中纯化重组小鼠Nanog蛋白的方法，为Nanog蛋白的活性检测及其基因表达调控机理的功能研究奠定基础。

For instance, ROS is involved in the hypersensitive responses, the lignification of the cell wall, the linkage of proteins and the inducible expression of many genes.

在植物抵御病原微生物的侵染过程中，ROS发挥了重要的作用，如参与超敏反应、细胞壁的木质化、富含经脯氨酸糖蛋白的交联、诱导表达抗病基因及 POD等病程相关蛋白。

Bone morphogenetic protein gene in bone tissue engineering for bone defect has become a new hot study currently.

骨组织中的骨形态发生蛋白以及骨形态发生蛋白的表达和功能由于他们显著的生物学作用而被长时间的研究。

Nighty rats were randomly divided into the groups of control, myocardial infarction and doxycycline, which were given orally 48 hours before and 48 hours after MI with 30mg/Kg per day. Protein and mRNA extraction was done on left ventricular samples containing scar and myocardium together. Samples were assayed from 1 day to 4 weeks post-Mi. The activity of MMPs was measured by zymography and collagen amount by the method of chloramine T, the ratio of II III collagen was assessed by immunohistochemical stain. Protein and mRNA of MMP-2, 9, TIMP-1 were determined by Western-Blot and RT-PCR.

分为对照组、心梗组和治疗组(D组，术前两天至术后两天，口服Doxycycline，30mg／kg／天)，分别于术后第一天、术后一周、术后两周、术后四周取心肌组织，采用免疫组化测定胶原含量和Ⅰ／Ⅲ胶原比例，酶谱法测定心梗后MMP-2，9活性蛋白的表达规律，Western Blotting进一步确定酶谱法中所消化条带蛋白的属性，逆转录-聚合链反应法测定心梗后MMP-2，9和TIMP-1的mRNA的变化规律，运用Acuson Sequoia 512超声心动图机分别测定心梗组、治疗组在两周和四周时左室前壁、后壁厚度以及左心室舒张末内径和左室射血分数。

VEGF protein was induced by hypoxia in rat cerebral microvascular endothelial cells, and NYA could upregulate the expression of VEGF protein, which may be one of the protection mechanisms for cerebral microvascular endothelial cells.

缺氧可诱导脑微血管内皮细胞VEGF蛋白的表达；脑溢安可以上调VECF蛋白的表达，这可能是脑溢安对缺氧的脑微血管内皮细胞的保护作用机制之一。

Ultimately, vulnerable groups of neurons atrophy and die in a process that may involve excitotoxicity (overstimulation of neurotransmitter receptors on neuronal surface membranes), collapse of calcium homeostasis, inflammation, and depletion of energy and growth factors. A form of apolipoprotein E, apoE4, contributes to the abnormal accumulation of A and tau, but probably also damages mitochondria and the cellular cytoskeleton. A , tau, apoE and -synuclein interact with many other molecules and modulate diverse signalling cascades that regulate neuronal activity and survival.

最后，易感神经元会萎缩直至死亡，原因可能有兴奋性中毒（神经递质对神经元表面受体的过度刺激引起），钙稳态失衡，炎症反应，能量和生长因子的耗尽。apoE4的产生可导致A肽和tau蛋白的异常聚合，可能也损害线粒体和细胞骨架。A肽、tau蛋白、apoE和突触核蛋白可与很多分子反应，也可调节不同信号级联反应，这些级联反应对于维持神经元活动性和生存性意义重大。

Primary sequence analysis and in vitro transcription/translation of ECPsp have revealed that it is a potential substrate for human signal peptide peptidase, an intramembrane protease located in endoplasmic reticulum.

此外，以小型干扰核醣核酸的方式压制人类蛋白质前导序列水解脢的表现后，表现嗜伊红血球阳离子蛋白前导序列融合蛋白的人类上皮肿瘤细胞也出现生长抑制的情形。

Combination of phosphoprotein enrichment and two-dimensional liquid chromatographic fractionation is an effective approach of phosphoproteomic studies.

磷酸化蛋白纯化技术与二维液相色谱技术的结合是研究磷酸化蛋白质组的有效方法，为下一步鉴定和研究磷酸化蛋白的功能打下坚实的基础。

By interacting with these proteins, PIAS proteins could affect their activation and function, and take part in gene transcriptional regulation and cellular signal pathway including Signal transducer and activator of transcription, Wnt, TGFβ, NF-κB pathways and so on, therefore present important biological functions.

通过相互作用，PIAS蛋白通过影响了蛋白的活性和功能，参与基因的转录调控和包括STAT(Signal transducer and activator of transcription)， Wnt， TGFβ， NF-κB等通路在内的细胞信号通路，具有重要的生理功能。

EST similarity analysis was finished by comparing sequences with BLASTx searches in NCBI. The results showed that they are proteins involved in biological processes such as energy metabolism and RNA molecular level regulation. Some of them are enzymes and structural proteins in the cell. This study provides useful information for future studies on molecular mechanism of filament coloring and for breeding new silkworm variety that spin color cocoons.

通过BLAST比对和对所得表达序列标签的同源分析表明，这些基因主要涉及能量代谢因子、RNA分子水平相关调节因子、酶类、结构蛋白等，由此初步探明了参与黄血基因协同作用的相关基因表达蛋白的种类和数量，可为进一步研究蚕茧着色的分子机制及通过遗传选择培育天然有色茧品种提供基础信息。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。