蛋白的

OrLPT1 ORF encods a polypeptide with 535 amino acid residues (molecular mass of 57.6KDaltons), which shares more than 64% amino acid identity with some other high-affinity Pi transporters of plants. Hydropathy analysis of the deduced polypeptides showed that the OrLPT1 is highly hydrophobic and contains 12 putative membrane-spanning regions.

OrLPT1 ORF 编码长为535 个氨基酸(分子量57.6 KDaltons)的蛋白，与其他植物物种磷酸盐转运蛋白的同源性在64%以上；多肽链的疏水性分析表明，OrLPT1 具有12 个疏水的跨膜区及高亲和力磷酸盐转运蛋白中保守的3 个磷酸化作用位点：蛋白激酶C作用位点、N端糖基化部位和酪蛋白激酶II 作用部位； 3。

In the second trial, five groups of diets with isonitrigenous (40% protein) and isoenergetic 20 MIJg^(-1 gross energy and different relativity of essential amino acid balance with soybean protein replacing 0.0%, 13.5%, 27.0%, 40.5% and 54% of fish meal protein, were formulated to determine optimal replacement level of soybean protein to fish meal protein.

实验Ⅰ以褐鱼粉为蛋白源，配制5个蛋白水平(31.04%、35.51%、40.89%、46.62%、50.33%)的等能、等必需氨基酸平衡关联度的半精制饲料，探讨翘嘴红鲌对饲料蛋白的需求；经过8周饲养，实验Ⅰ的结果表明，饲料蛋白含量对翘嘴红鲌的增重率、饲料效率和蛋白效率具有显著影响。

①The collected clinical Acinetobacter baumannii isolates were more seriously resistable to Ciprofloxacin than to Imipenem;②The antibiotic resistance of clinical Acinetobacter baumannii isolates may be associated with the decline of CarO protein expression;③There is some relationship between CarO protein and ciprofloxacin-resistant Acinetobacter baumannii strains,it proves that CarO protein supports bacteria on multi-drug resistance;④CarO protein may be related to cell signalling;⑤The difference in CarO protein functional sites and structure between the sensitive strains and the resistant strains may be related to bacterial resistance.

①收集的临床鲍曼不动杆菌对环丙沙星的耐药情况较亚胺培南严重；②CarO蛋白的表达下调可能与临床鲍曼不动杆菌的耐药性相关；③CarO蛋白与鲍曼不动杆菌耐环丙沙星有一定相关性，佐证了CarO蛋白可能与细菌多重耐药有关；④CarO蛋白可能与细胞信号传导有关；⑤敏感菌株与耐药菌株的CarO蛋白功能位点和内部结构有差异，可能与细菌耐药有关。

One of them was identified as aldehyde dehydrogenase 2, and the others were both identified as mouse RIKEN cDNA 2900053E13, suggesting that the protein might be post translationally modified.

通过对差异蛋白的胶内酶解和高效液相色谱／离子阱质谱鉴定，发现其中一个蛋白点为乙醛脱氢酶2（aldehyde dehydrogenase 2），另外两个差异点鉴定结果为同一个蛋白RIKEN cDNA 2900053E13，提示该蛋白在正常和心肌缺血心肌的线粒体内修饰状态发生了变化。

After the sequence and open reading frame were confirmed by DNA sequencing, the GADes gene was expressed in E.coli. SDS-PAGE analysis suggested that the bacteria BL21 produce a kind of fusion protein of 92KD and the expressed GADes fusion protein shows expected immunoreactivity by Western Blot.

SDS-PAGE电泳和Western Blot分别鉴定融合蛋白大小和免疫活性后，对表达条件进行优化，对包涵体进行分离、溶解和复性处理，以提高可溶性融合蛋白的产量，使融合蛋白达总蛋白的50％，相当于1.495g/每升培养物。

No immunostaining was seen in RPE. Double staining that Kir2. 1 and glutamine synthetase, Kir2. 1 and glutamine synthetase colocalized well. Intense Kir7. 1 immunolabeling was present on the apical surface of all RPE cells and appeared to extend over the length of the apical processes. Na〓, K〓-ATPase expression varied among RPE cells, but in highly expressing cells, it co-localized with Kir7. 1. Immunoreactivity of Kir2. 1、Kir4. 1 and Kir7. 1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine.

间接免疫荧光组织化学显示，Kir2.1蛋白主要分布在Müller细胞与神经元相接触的细胞膜区域；与Müller细胞的特异性标记蛋白质抗谷氨酸合成酶抗体双重标记显示其分布特性重叠，在RPE未检测Kir2.1蛋白的免疫活性存在；Kir4.1蛋白集中分布于Müller细胞的内侧突起，与GS蛋白双重标记显示其分布特性重叠，RPE未检测Kir4.1蛋白的免疫活性存在；Kir7.1主要分布于RPE游离面及其突起的全长，与特异性标记RPE突起的Ezrin蛋白完全重叠，Na〓-K〓ATP酶在不同部位的RPE表达不同，Na〓-K〓ATP高表达的RPE细胞与Kir7.1的分布特性重叠。

In mammals the Notch gene family comprise four related genes, Notch1-4, as the Notch ligands that encode transmembrane proteins. Ligand-receptor interaction mediated Notch activation ultimately leads to the conversion of effectors-CSL CBF1, Su(H, Lag-1 proteins, from repressors to transcriptional activators, and subsequent up-regulation of downstream targets.

在哺乳动物中，Notch基因家族包含了4个相关的基因，分别为Notch-1至-4，和Notch受质蛋白一样能被转译成膜蛋白；受质-受体间相互作用所引起Notch受体蛋白的活化会促使作用蛋白-CSL CBF1， Su(H， Lag-1 蛋白扮演抑制分子或者是转录活化分子上的角色改变，并且进一步地正向调节下游的标的基因。

BST16 was also proved to encode gene of PSII lOkD protein by protein prosite and conserve domain analysis. The protein encoded by BST16 contained a conserve domain PsbR of PSII lOkD protein from 48 to 140 and its carboxy terminal had an ADH_SHORT prosite of Short-chain dehydrogenases/reductases family signature from 94 to 122, which showed the protein encoded by BST16 would be a reductases.

对该基因可读框架编码的蛋白进行功能位点和结构功能域的分析，同样证明了该基因为PSⅡ 10kD蛋白编码基因，其氨基酸摘要序列 48刁位含有一个保守的光合系统＊ 10kD蛋白结构域 PSbR，在蛋白的猿基端有一个保守的短链脱氢酶／还原酶家族特征序列ADHSHORT（Short－chain dehydrogenases／reductases family signature），位于94－122位，说明了该蛋白可能具有还原酶活性。

" As a result,"the primary focus should be on LDL, said review co-author Mehdi Shishehbor, D.O., of the Cleveland Clinic.

作者的结论是，尽管努力降低低密度脂蛋白(低密度脂蛋白或&坏胆固醇&)&不断降低心血管疾病风险，高密度脂蛋白的做法要复杂得多，有时令人失望&作为一个结果，&应该把主要焦点对低密度脂蛋白，说：&检讨合著梅迪shishehbor ， d.o。，克利夫兰诊所。

The choosing of the material and staining of Golgi body and making section;2. To determine the subcellular localization of the encoded protein of a transcriptionally up-regulated gene pdd87in the pten knockout mice,fusion protein expression vector pEGFP-C 1 -pdd87was constructed and transfected into NIH3T3cells and cells were stained with BODIPY TR C 5 -Ceramide to display Golgi body .

为了明确pten敲除小鼠中转录上调基因pdd87编码蛋白在细胞中的定位，构建PDD87与绿色荧光蛋白的融合蛋白表达载体pEGFP-C1-pdd87，利用Lipofectamine2000转染该载体于体外培养的NIH3T3细胞中，采用高尔基体特异探针BODIPYTRC5-Ceramide染色显示高尔基体，激光共聚焦显微镜下观察到PDD87-GFP融合蛋白定位于高尔基体，提示pdd87基因编码的蛋白定位于高尔基体。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。