蛋白的

The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3.1中，成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后，通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

Results: The expression of both mRNA and protein of c-myc were obvious in the bud and cap stages,while decreased in the bell stage, then increased in ameloblast and dentinoblast when dentin formation. The expressions of c-myc were stronger in mesenchymal cells than in epithelial cells. The expression and distribution of c-myc mRNA is consistent with that of c-myc protein.

结果：c-myc mRNA及蛋白的表达在蕾状和帽状期明显，钟状期减弱，而牙体组织形成期成釉细胞、成牙本质细胞及间充质牙乳头细胞表达又增强。c-myc蛋白表达和分布与c-myc mRNA转录基本一致。

In whole growing stage, organic fertilizer can increase the soluble sugar and soluble protein content, especially in anaphase.

整个生育阶段，有机肥有效地提高了可溶性糖和蛋白的含量，特别是后期可溶性糖和蛋白含量。

Water, Glycerin, Methylsilanol Mannuronate, Sodium Carboxymethyl Beta-Glucan, Sodium Hyaluronate, Palmitoyl Oligopeptide, Palmitoyl Tetrapeptide-7, Madecassoside, EGF *, FGF *, Telomerase *, Panthenol, Borago Officinalis Seed Oil, Anthemis Nobilis Flower Extract, Tocopherol, Tocopheryl Acetate, Cetyl Palmitate, Citrus Aurantium Dulcis Peel Oil, Citral, Citronellol, Limonene, Linalool, Zea Mays Starch, Butylene Glycol, PEG-40 Hydrogenated Castor Oil, Hydrolyzed Corn Starch, Hydrolyzed Corn Starch Octenylsuccinate, PEG-33, PEG-8 Dimethicone, Hydroxyethylcellulose, PEG-14, Xanthan Gum, Carbomer, Polysorbate 20, Caprylyl Glycol, Phenoxyethanol, Methylparaben.

主要有效成分是：聚羧基甲基β葡萄糖的钠盐，保湿锁水；透明质酸，高效保湿；寡胜肽；四胜肽；羟基积雪草甙，抗炎症，促进胶原蛋白的合成；表皮生长因子EGF，促进细胞新陈代谢；成纤维细胞生长因子FGF，促进胶原蛋白合成；端立酶，一种RNA与蛋白的复合体，可通过合成新的端粒而修补受损的DNA端粒，使细胞持续不停的增生，也就是利用成人干细胞转变为全新的细胞。维他命B5；琉璃苣籽油，含维他命和矿物质；洋柑橘萃取液，舒缓，抗过敏；维生素E；醋酸盐维生素E；橙皮油；水解玉米淀粉。

AIM To evaluate the mechanism that natural anti-keratin autoantibody inhibits the proliferation of keratinocytes and squamous cell carcinoma.

目的 探索天然抗角蛋白自身抗体对培养角质形成细胞及鳞癌细胞凋亡相关蛋白的表达影响。

There is close relation between BiP protein expression and increased functions of splenic macrophages induced by mild heat stress.

轻度热应激脾脏巨噬细胞BiP mRNA、BiP蛋白的表达与巨噬细胞功能发生同步改变，提示BiP蛋白表达上调可能与轻度热应激脾脏巨噬细胞功能增强密切相关。

The enzymolysis effect of neutral protease, papain, pepsin, bromelin, flavourzyme and compound protease was studied respectively on the sleeve-fish protein.

探讨了中性蛋白酶、木瓜蛋白酶、胃蛋白酶、风味蛋白、酶菠萝蛋白酶以及复合蛋白酶对鱿鱼蛋白的酶解效果。

Effects of microbial transglutaminase on the tensile properties of plastic made from soy protein isolates are studied. The results show that the tensile strength and Young modulus of the protein plastic are increased from 9.2MPa and 265.4MPa of the control, which is not treated with TG, to 14.7MPa and 390.1Mpa respective, which is treated with TG at 50℃ for 60min. High molecular weight protein conjugates are formed with TG treatment, and they are responsible for the enhanced tensile properties, denaturalizing temperature and enthalpy values.

研究了微生物谷氨酰氨转胺酶对大豆分离蛋白塑料拉伸性能的影响，发现在50℃时转氨酶对大豆分离蛋白催化聚合反应60 min，形成的高分子聚合物使大豆分离蛋白的抗拉强度和杨氏模量分别由未经处理时的9.2 MPa和265.4 MPa增加到14.7 MPa和390.1 MPa，同时增加了蛋白质的变性温度和热焓值。

II. Exploring non-radioactivc method to select the optimal DNA-bindingsequencc fOr singIe-chain reprcssorCloning and expression of single-chain repressors with cysteine tai1 wereperformed and immobilized these protein in the solid surface by using the standardmicrowell plates Which have been activated to contain maleimide grouPs, al1owing tobind sulthydryl-containing protein on its surface.

二。非同位素的方法筛选单链阻遏蛋白的最佳DNA结合序列初探克隆和表达了带半眈氨酸尾的单链阻遏蛋白，利用己包彼了马来酚胺的活性板可以与自由琉基结合的特性，将蛋白固定在活性板表面。

As estimated by SOS-PAGE the molecular weight of PG was 31500, lower than that of PG in other fruits. The most pH stability was at a range of pH 4.5-8.0. The most optimum ion strength was at 0.O4mol/L-0.O6mol/L. The U.V. spectrum of PG was about 267 nm.

经SDS-PAGE测得枣PG蛋白的分子量是31500，比其它果实中的PG蛋白的分子量小；PG蛋白的pH稳定范围是4.5-8.0；最适离子强度范围是0.04 mol/L-0.06mol/L；PG酶的最大紫外吸收光谱是267nm。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。