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To realize the molecular compositions and bio-spinning mechanisms of spidroin in spider major ampullate, the molecular weight and amino acid compositions of spidrion solution were measured and compared with that of dragline silk. The molecular conformations and morphologies of spidroin and dragline silk in different spinning phases were determined by circular dichroism spectroscopy and laser Raman spectroscopy.

本文以广泛分布于我国各地的大腹园蛛为对象,研究了大腹园蛛主腺体内丝蛋白溶液的分子量和氨基酸组成,并利用圆二色光谱和拉曼光谱分析了蜘蛛在不同成丝阶段丝蛋白的分子构象和聚集态结构的变化,以期了解蜘蛛主腺体内丝蛋白的分子组成及其生物纺丝机制。

The method uses radiative immunity analysis, determine 43 senile domentia and 33 hemal sexes are gawkish (the β in VD) patient and plasma of 58 healthy old people - the content; analysis of amylaceous appearance albumen and β of observation AD patient - the variable relation of the content of amylaceous appearance albumen and GDS process is reached and treat from beginning to end.

方法应用放射免疫分析,测定43例老年性痴呆和33例血管性痴呆患者及58例健康老年人血浆中的β-淀粉样蛋白的含量;分析和观察AD患者β-淀粉样蛋白的含量和GDS进程的变量关系及和治疗前后β-淀粉样蛋白含量的变化。

There were on unique different protein observed in flag leaf at different development stage; but unique different protein were found in anther at binucleated stage and the male-sterile materials lack a 31 kD polypeptide compared with normal material.

结果表明,从不同发育时期的旗叶可溶性蛋白中尚未发现特异蛋白的存在,但在花药中发现有特异蛋白的存在,表现在二核期生理型雄性不育界遗传型雄性不育材料均比正常的可育材料缺少了1条分子量为31kD的多肽。

The propagation mechanism of the prion diseases is widely accepted that PrPsc transforms PrPc into the pathological form and more and more PrPsc will gradually be produced and forms larger fibrillar aggregates.

羊瘙痒症是最早发现的朊蛋白病,而疯牛病在英国的流行以及新近发现的新变种的人类雅各布氏病引起研究者们对朊蛋白疾病及其致病蛋白的更大关注。

By the bioinformatics analyzing tools in bioinformatics webs site such as NCBI (http:///www.ncbi.nlm.nih.gov/), ExPaSy (http://www.expasy.org/) and combining else complicated bioinformatics software package ,to identify a EF-1 full-length gene from the Taenia asiatica eDNA plasmid libratory and predict the structure and function of the protein.

方法利用生物信息网站如美国国家生物技术信息中心(NCBI,http:∥www.ncbi.nlm.nih.gov/)和瑞士生物信息学研究所的蛋白分析专家系统(ExPASY,http:∥ca.expasy.org/)中有关基因和蛋白的序列和结构信息分析的各种工具,结合其它生物信息学分析软件包,从亚洲带绦虫成虫全长cDNA质粒文库中识别延伸因子-1的全长编码基因并预测其蛋白的结构和功能。

In addition, down-regulation of Smad3 protein might be a concomitant event during the inhibition of myogenic differentiation by hypoxia.

随后我们研究低氧抑制成肌细胞体外分化过程中Smad3蛋白水平降低的原因时发现,Smad3的活性形式磷酸化Smad3蛋白的表达水平在低氧抑制成肌细胞体外分化的过程中下调,与Smad3蛋白水平的降低趋势相平行。

The results indicated that the mutant protein promoted the survival of dorsal root ganglion、induced TF-1 prolification and made the normal mice lose weight,decrease appetite and reduce fat index.The weight loss effect was dependant with its administration dosage,ED50 was 150.986?

结果是突变体蛋白能促进鸡胚背根神经节的生长;促进TF-1细胞增殖,MTT测定法表明突变体蛋白与国际参考品相比,比活不低于2.0×106U/mg;使正常小鼠的体重减轻,摄食量减少,脂肪指数下降,并且体重的减轻与突变体蛋白的给药剂量呈现良好的剂量依赖关系,其ED50为:150.986?

These results also provedthe presence of the same acute phase response profile in amphioxus,as observed ineutherians.Thus it pushed the evolutionary existence of the eutherian-like acute phaseresponse back to the protochordate lineage.Fourthly,we determined CAs,including epinephrine、norepinephrin anddopamine,with HPLC method optimized after LPS challenged amphioxus.Theresults demonstrated that E,DE and DA are present in amphioxus humoral fluid,andE was firstly found in amphioxus.

第三,我们用生物化学方法,选取海洋中广泛存在、众多水产动物的常见致病菌-溶藻弧菌作为天然反应原感染文昌鱼,从另外一个角度探讨并丰富文昌鱼体液中的八种炎性蛋白的急性时相反应,结果显示,与LPS感染文昌鱼相比,六种正APPs的浓度变化幅度相对较小,曲线相对平缓,但仍然呈正急性时相反应,蛋白电泳也证实了这一点;而二种负APPs:RBP和白蛋白(Albumin,ALB)浓度实验周期内总体呈下降的趋势,呈负急性时相反应,结论与上述LPS相同。

In this study, the deletion mutants of SulA with regard to C-terminus were constructed and the interaction with ClpY was analyzed in yeast two-hybrid system.

本实验中,为确认ClpYQ蛋白酶辨认SulA蛋白的区域,建构SulA之C-端不同大小片段缺失的突变蛋白,以酵母菌双杂交系统测试,各个SulA突变蛋白与ClpY之间交互作用的情形,发现ClpY辨识的区域可能位於C-端的高疏水性片段,C-端第20 - 30个胺基酸。

Tobacco mosaic virus, a member of alphavirus-like superfamily, the N-terminal truncated TMV helicase formed hexamer and exhibited double strand RNA unwinding activity. Accordingly, we also constructed the same N-terminal truncated BaMV helicase-like domain and analysis the capability to unwind double-strand RNA.

在同属於alphavirus-like superfamily的Tobacco mosaic virus之解螺旋蛋白的分析中,其N端的截断蛋白会形成六聚体,而且具有解旋活性,因此我们同样建构了许多类解螺旋酵素N端的截断蛋白,测试其蛋白质的聚集形式及解旋活性。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。