蛋白的

Besides, we also found that NOD2 could participate in down-regulation of macrophage bactericidal activity and TLR4-induced LC3 activation.

在另外的实验中，我们也发现NOD2蛋白能够负向调控巨噬细胞的杀菌能力以及TLR4所引发的LC3蛋白的活化(是一个细胞自噬的标志蛋白)。

In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.

特别是，设计一种蛋白以表现出对鞘翅目害虫提高的毒性，表达较高水平的这种蛋白的转基因玉米表现出优越的昆虫防治水平，并不太可能引起产生抗所述杀虫活性蛋白的目标昆虫群体。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

Based on the biochemical analysis of the two proteins in vitro, the results of yeast two-hybrid experiment and protein-interaction in both wild type cells and C48 strain, we conclude HetR can form a homodimer through the Cys48 residue. We also show that the dimerization is required for heterocyst differentiation.

基于体外两种蛋白的性质比较和酵母双杂交的结果，并结合相应蓝细菌野生型和突变株C48A中HetR蛋白性质的体内研究表明：HetR蛋白可以通过第48位半胱氨酸残基形成共价结合的同源二聚体，该同源二聚体是异型胞正常分化所必需的。

The extracellular edelfosine promoted the phosphatization of Spmlvia inducing the expression of Mid2; The intracellular edelfosine increased the phosphatization of Spmlvia inhibiting the expression of Pmp1, and leading the holdback of cytokinesis.

细胞外依地福新诱导Mid2蛋白的表达，从而促进的Spm1磷酸化；细胞内依地福新通过抑制Pmp1蛋白的表达，取消Pmp1蛋白对Spm1的抑制作用，使Spm1的磷酸化程度增加，最终导致粟酒裂殖酵母细胞胞质分裂障碍。

Then, the experiment that edelfosine inhibited the phosphatization of Spm1 was carried out in order to elucidate whether edelfosine affect on the phosphatization of Spm1 induced via Mid2 and inhibited via Pmp1.(1)、On the inhibition experiment of parallel growth of S. pombe wild-type cells, spm1 mutants, pmp1 mutants, mid2 mutants and relevant retransform strains, the spm1 mutants grew well treated with 5.0μM and 10.0μM edelfosine for 24 h; the growth ratio of spm1 mutants had a statistic significance in between the cells previously mentioned and the S.

(1)、通过野生型粟酒裂殖酵母和胞质分裂突变体spm1Δ、pmp1Δ、mid2Δ及其再转化株对依地福新的平行生长抵抗试验，进一步确定依地福新对spm1、pmp1、mid2基因的影响；(2)、应用依地福新对Spm1磷酸化的影响试验，阐明依地福新是否通过影响Mid2蛋白和Pmp1蛋白的表达而影响Spm1蛋白的磷酸化。

In order to explore the possibly involved morphological mechanism of R phycoerythrin mediated photodynamic killing at cell level, the fluorescence distribution of R phycoerythrin——a novel photosensitizer in tumor cells——was observed.

在荧光显微镜下观察新型光敏剂R藻红蛋白的荧光在肿瘤细胞中的分布，探讨以藻红蛋白为光敏药物的光动力损伤可能发生的细胞水平机制，以及培养液酸度和藻红蛋白浓度对其与细胞结合、进入及光动力杀伤的关系。

Yam steroid saponins and sapogenin are resulting in MCF-7 cells apoptosis by arrested the G0/G1 phase, increased the p53 protein with promoting apoptosis and decreased the AKT protein with anti-apoptosis and the BRCA-1 protein with repairing DNA. And, SPG has the excellent anti-cancer effect on MCF-7 cells may be due to induced much of the p53 protein in the early treated stage.

进一步的研究发现，山药固醇类皂及皂元抑制MCF-7乳癌细胞的功效，主要是诱发G0/G1期停滞，造成细胞凋亡；其透过的机制是经由p53促凋亡蛋白的增加，及AKT与BRCA-1等抗凋亡蛋白被抑制，达到双重细胞凋亡之抗癌效果；其中SPG处理之MCF-7肺癌细胞具早期大量诱发p53蛋白的特性，或许是SPG优异抗癌效果之原因。

The content variation to Chrysopa septempunctata"s vitellin was detected through ELISA. It indicated that vitellin was utilized gradually during embryonic development, and a fallowing trend in form of straightline was found in the whole embryonic development. Vitellin couldn"t be detected in just hatched larvae.

通过ELISA来测定大草蛉的胚胎发育和胚后发育中卵黄蛋白的含量变化，随着胚胎的发育，卵黄蛋白逐渐被利用，在整个胚胎发育过程中呈直线下降趋势，在刚孵化的幼虫中已检测不到卵黄蛋白的存在。

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。