蛋白的

Clau (5, 10, 20〓) was bound to rat plasma a greater extent than clau, while in rabbit plasma the reverse was the case. By Scatchard plots using bovine serum albumin, clau had a higher equilibrium constant and the greater numbers of binding sites than its antipode.

在与大鼠血浆蛋白的结合中，clau的结合率平均为28.37±0.31（n=3，三个剂量5，10，20μM），clau的结合率平均为38.05±2.61（n=3），clau结合率低于clau；而在与兔血浆蛋白的结合中，clau的结合率平均为47.21±4.91（n=3，三个剂量），clau的结合率平均为41.43±5.21（n=3），clau的结合率略高于clau；在与牛血清白蛋白的结合中，clau的结合率也高于clau，立体选择方向与兔血浆蛋白结合相同，而与大鼠血浆蛋白结合相反，提示存在种属差异；在与系列浓度的牛血清白蛋白结合中，通过Scatchard作图法求得蛋白结合点数和平衡常数；在clau，N和K分别为90.02±35.76和5.326±1.196（n=4）；在clau，N和K分别为59.92±20.39和4.203±1.250（n=4），两者的比值分别为1.50和1.27；进一步说明clau对映体的蛋白结合存在立体选择性差异。

Finally, the fusion protein was expressed in E coli DH5α under induction by arabinose. And the fusion protein was 65 kD and account for 30% of the total protein of the bacteria.

SDS-PAGE电泳分析显示，融合蛋白分子量约65 kD，与预期蛋白分子量一致，并且目的蛋白占菌体蛋白的30%。

After developing 48 hours, hepatocytes enters 2.0 mM t-butyl hydroperxide or 2.0 mM diamide to induced cell blebbing. The samples collected at 0, 15, 30, 60 minutes to analyze cell about membrane protein thiol deletion; the leakage of lactate dehydrodenase; the change of glutathione; the lipid peroxidation; the variation of actin and tubulin, and use Confocal fluorescent microscope to observe the change of actin and tubulin in rat hepatocytes.

肝细胞在48小时培养后，分别加入2.0mM t-buty1 hydroperoxide或2.0 mM diamide诱发细胞膜小泡生成，并分别於0、15、30、60分钟取样，分析细胞膜硫醇流失程度；乳酸去氢□渗漏；□胱甘□浓度变化；脂质过氧化；肌动蛋白与管蛋白变化；并利用雷射共轭焦萤光显微镜观察肝细胞中肌动蛋白及管蛋白的变化。

Based on electro-characteristic and double electric layers models, directional mechanisms of soy protein molecular are analyzed for the first time on DC heating condition. After the main factors which effect soy protein gels transparency and strength being studied on DC heating condition, the different affects on soy protein gels transparency and strength by DC heating and water bath heating are analyzed and compared, and the transparent mechanisms of soy protein gels are further explored. In addition to temperature action as water bath heating, DC heating is added as electric field directional action. As a results, heating rate is increased. Directional range degrees of soy protein molecules are raised. These advantage the increase of soy protein gels transparency and strength. Microstructures of soy protein gels forming on DC heating condition are observed by SEM and the results showed that ordered structure of transparent soy protein gels on DC heating condition is more ordered than that of transparent soy protein gels on water bath heating condition.

首次从大豆蛋白的电特性和双电层模型上分析了直流电加热条件下，大豆蛋白分子定向的机理；通过对直流电加热条件下影响大豆蛋白凝胶透明性和强度主要因素的研究，分析和比较了直流电加热与水浴加热对大豆蛋白凝胶透明性和凝胶强度的不同影响，又进一步探讨了大豆蛋白凝胶透明的机理；直流电加热除了水浴加热中的温度作用以外，又增加了直流电场的定向作用，提高了加热速率，提高了大豆蛋白分子定向排列的程度，有利于蛋白质凝胶透明性和强度的提高；利用SEM对电场下大豆蛋白凝胶微观结构的观察，进一步证明了直流电加热比水浴加热形成的透明大豆蛋白凝胶微观结构具有更高的有序性。

In the paper, processability of different proteins was investigated and the water retentiveness, emulsifiability and thermal stability of soybean separation protein, animal protein, lactalbumin, casein, and glutenin were compared.

研究了不同蛋白质的加工性能，比较了大豆分离蛋白、动物蛋白、乳清蛋白、酪蛋白、小麦蛋白的持水性、乳化性和热稳定性。

In its protein has the albumin,谷蛋白, mellowly dissolves theprotein, the amino acid and so on, always tonic which prolongs thelife by our country people achievement.

其蛋白质中有白蛋白、谷蛋白、醇溶蛋白、氨基酸等，历来被我国人民作为延年益寿的补品。

Liver function and pathological changes of hepatic tissue were examined.

采用ANIT灌胃制备大鼠急性肝内胆汁淤积病理模型，共32只大鼠，设正常组、正常＋大黄素组、模型组和模型＋大黄素组，观察各组实验动物肝功能各项生化指标和肝组织病理学改变，并采用实时荧光定量RT-PCR检测肝细胞膜转运蛋白基因多药耐药蛋白1a（mdr1a）、多药耐药蛋白1b（mdr1b）和多药耐药蛋白2（mdr2）mRNA水平的表达，Western blot方法检测肝脏P-糖蛋白的变化。

We isolate the LCA binding glycosylated proteins from anosia human liver cell extraction and liver cancer cell extraction by LCA coupled epoxy magnetic particles, and the glycosylated fractions were separated by 2D gels which were analyzed by 2D ImageMaster to established disparity expression spectra between Chang Liver and SMMC-7721 firstly.

利用该方法对健康人肝脏细胞Chang Liver总蛋白、肝癌细胞SMMC-7721总蛋白及健康人血清进行了糖蛋白的分离纯化，结合2D电泳技术及其图像分析，建立了健康人肝脏细胞ChangLiver和肝癌细胞SMMS-7721 LCA结合糖蛋白的差异表达谱，分别得到蛋白质点148±5和209±7个，匹配点数为127，匹配率为85.36%。

Nucleoprotein is also a major immunogenetic antigen for SARS; however, several tests have shown that N protein had the reactivity with other coronavirus positive sera. It is therefore an advantage of S protein as recombinant target antigen for SARS serum diagnosis compared with recombinant N protein. Moreover, S protein has been shown to be the major antigenic determinant that induces neutralizing antibodies and protective immunity to SARS-CoV, so it is also important in designing SARS genetic engineering vaccine.

SARS-CoV S 蛋白由1255 个氨基酸组成，体外表达如此大的蛋白很困难，而且表达蛋白的可溶性不高；其次，实验已经证实，全长S 蛋白含有大量无关的表位，致使表达的全长S蛋白对SARS 阳性血清的检出率低于S 蛋白片段；另外作为疫苗研究，S 蛋白上大量无关表位作为异体蛋白进入体内，会引发机体的过度免疫反应，这也是SARS-CoV 可能的致病机理之一。

With an increase in Bcl-2 protein, more Bax homodimers were separated and conjugated with Bcl-2 protein to form more stable Bcl-2/Bax heterodimers than Bax homodimers, which'neutralize'apoptosis induction of Bax protein.

随着Bcl-2蛋白增多，越来越多的Bax蛋白同源二聚体被分开，与Bcl-2蛋白结合形成比Bax-Bax蛋白更稳定的Bcl-2-Bax蛋白杂二聚体，从而&中和&Bax蛋白的诱导细胞调亡作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。