蛋白的

The adenovirus plasmid was identified by PacI digestion and transfected into 293A cells to package a recombinant adenovirus which expressed the Fhit protein. Furthermore, the adenovirus rAd-Fhit was infected into colon cancer cells,and the expression of the ectogenic protein was detected by Western blotting. Finally, the proliferation of colon cancer cells was observed in adenovirus-infected cells by the MTT assay.

经PacI酶切鉴定正确后，将重组腺病毒质粒转染293A细胞获得表达Fhit蛋白的重组腺病毒rAd-Fhit，将获得的重组腺病毒感染结肠癌细胞，采用蛋白印迹法检测外源Fhit蛋白的表达，并进一步观察其对细胞增殖能力的影响。

Parasite nucleic acid vaccine,a new type of vaccine emerging and developing in recent years,can induce not only humoral immunity,but also a high level of cellular immune responses.Parasite DNA vaccine displayed a great advantage in the prevention of parasitosis.

核酸疫苗(nueleie aeid vaeeine，DNA vaeeine)，也常被称为基因疫苗是指将编码某种抗原蛋白的外源基因直接导人动物细胞内，并通过宿主细胞的表达系统合成抗原蛋白，诱导宿主产生对该抗原蛋白的免疫应答，以达到预防和治疗疾病的目的。

The structure, photosensitization of phycocyanin and the principles of photodynamic therapy were mainly reviewed.

本文阐述了藻蓝蛋白的结构、光动力疗法的原理和藻蓝蛋白光敏作用的研究进展，并介绍了藻蓝蛋白在光动力疗法中的应用现状与前景。

Simultaneously, according to different target proteins, these two mechanisms of PIAS proteins are not absolutely repulsive, which indicates the specificity and complexity of the function of PIAS proteins.

同时，针对不同的靶蛋白，PIAS蛋白的上述两种作用机制并不是完全互相排斥的，这体现了PIAS蛋白功能的特异性和复杂性。

As a result, Core gene was expressed in E.coli with yields of 20% of total proteins and it was a 31KD fusion protein on SDS-PAGE and Western-Blot, it shows that Core protein was highly expressed in E.coli;One fragment of 369bp could be seen on the gel of the Core gene PCR product. A band of 29KD could be seen on the Western-blot after induction of yeast containing pPICZaA-Core for 72 hours. This indicate that Core protein was secretively expressed in the Pichia pastoris with weak yields.

结果显示pBVIL1-Core的表达产物经SDS-PAGE分析出现一条约31KD的带，与预期融合蛋白的分子量相符，表达蛋白存在于包涵体中且表达量占菌体总蛋白的20%，Western-blot显示诱导后菌体在相应位置出现特异性杂交带，表明

Comparison of SRY/Sry primary structure of human and bovine, the HMG-box was the singular conserved part, but they were similar in the three-dimensional structure.

比较牛和人SRY蛋白结构，仅在保守区HMG-box处有较高的相似性，但是空间构象却比较相似，都是通过结合DNA螺旋小沟来识别7个碱基的核心靶序列，作用方式也很相似，说明虽然SRY/Sry蛋白在物种间序列差异较大，但蛋白的结构和作用方式却是很保守的。8。

Background— Numerous in itro studies suggest that sphingosine 1-phosphate (S1P), a bioactie lysosphingolipid associated with high-density lipoproteins, accounts at least partly for the potent antiinflammatory properties of high-density lipoprotein and, thereby, contributes to the antiatherogenic potential attributed to high-density lipoproteins.

背景——众多的活体研究表明鞘氨醇1-磷酸盐作为一种与高密度脂蛋白相关的生物活性溶血磷脂，至少是高密度脂蛋白有力抗炎特质的部分原因，从而促成高密度脂蛋白的抗动脉粥样硬化潜能。

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化，重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达，菌体密度最终达到115g/L以上，可溶性重组融合蛋白占菌体总蛋白的30%以上，含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养，结果表明该工艺能有效的放大，可适用于工业生产。

The result of homological analysis showed that an open reading frame,which contains 289 amino acids,is more similar to the Choristioneure fu- m...

比较多种核型多角体病毒的多角体膜蛋白和多角体蛋白的氨基酸序列发现，这两种多角体结构蛋白的同源性具有相似的变化趋势。

Antioxidant activity of Se-PC was investigated and compared with phycocyanin by using four different free radical scavenging assays, namely, trolox equivalent antioxidant capacity assay, DPPH free radical scavenging assay, superoxide anion scavenging assay and assay for erythrocyte hemolysis mediated by peroxyl free radicals .

通过含硒藻蓝蛋白与藻蓝蛋白对4种不同的自由基清除能力的比较，即总抗氧化能力、清除DPPH自由基、过氧化物阴离子和引发红细胞溶血的过氧化氢自由基能力测定，从而对含硒藻蓝蛋白的抗氧化活性进行评价。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。