蛋白的

The structure and expression pattern of each storage protein was investigated using one-dimensional SDS-PAGE. Our results showed that the protein content of each fraction in endosperm of autotetraploid rice line is mostly higher than that of its corresponding diploid rice line.

结果表明：同源四倍体水稻胚乳蛋白各组分含量与相应的二倍体相比，大部分呈增加趋势；同源四倍体水稻胚乳蛋白的亚基类型与相应的二倍体水稻基本一致，仅在全蛋白电泳和清、球蛋白电泳中各发现1条差异条带。

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

A recombinant protein of Bla g 2 has been obtained, which is soluble in the supernatant and therefore can avoid a process of denaturalization and renaturation of the recombinant.

获得了真核表达的德国小蠊主要变应原重组蛋白Bla g 2，该蛋白以可溶性蛋白的形式分泌到培养液中，避免了原核表达的变复性过程。

Methods:YCP was coupled to bovine serum albumin to construct three neoglycoprotein s which are different in their degrees of substitution(DSs;≈3,≈6,or ≈10 mol of BSA carrier/mol of YCP hapten,respectively),and their immunogenicities were evaluated in mice following subcutaneous immunization,respectively.

将YCP与牛血清白蛋白共价偶联，获得取代度分别约为3、6和10的3种拟糖蛋白，经皮下分别接种ICR小鼠，测定免疫血清中多糖YCP的特异性抗体IgM和总IgG水平，以及半抗原和载体蛋白的特异性IgG亚型。

A single chain protein with an apparent molecular weight of 33 kDa was purified from skin of Bombina maxima by a combination of ion exchange and gel filtration chromatography steps. N-terminal amino acid sequence determination indicated that it shares 70%, 64% and 56% identity with those of annexin Ⅱ from the African claw toad, red jungle fowl and human, respectively.

通过阴离子交换、凝胶过滤和阳离子交换层析，从大蹼铃蟾皮肤中纯化到一个表观分子量为33kDa的单链蛋白。N-末端序列比较分析显示，该蛋白与来自非洲爪蟾、红色原鸡和人膜联蛋白Ⅱ的N-末端序列相同的氨基酸分别占70％、64％和56％。

In search of databases, the deduced products of sanP, sanQ, sanR, sanS, sanT and sanU show highest similarity to those of nikP2, nikQ, nikR, nikS, nikT and nikU of S. tendae respectively. In comparison with the proteins of identified function, it is indicated that sanP encodes a thioesterase, sanQ encodes a cytochrome P450, sanR encodes a uracil phosphoribosyltransferase, sanS encodes a carboxylase, sanT encodes a histidinol-phosphate aminotransferase and sanU encodes a mutase.

在蛋白数据库中比较结果表明，sanP、sanQ、sanR、sanS、sanT和sanU基因编码的蛋白分别和唐德链霉菌的尼可霉素生物合成基因nikP2、nikQ、nikR、nikS、nikT、nikU六个基因编码的蛋白同源性最高；根据和已知功能蛋白的比较，推测sanP基因编码的是硫酯酶，sanQ基因编码的是细胞色素P450，sanR基因编码的是尿嘧啶磷酸核糖转移酶，sanS基因编码的是羧化酶，sanT基因编码的是组氨醇磷酸氨基转移酶，sanU基因编码的是一种变位酶。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

Fluorescence resonance energy transfer which based on green fluorescent protein is an important method to detect the interactions of proteins.

基于绿色荧光蛋白的荧光共振能量转移技术是探测活细胞中蛋白-蛋白相互作用的重要方法。

It is pointed out that chaperonin facilitates the correct refolding of recombinant protein and in turn increases the soluble protein.

分子伴侣蛋白帮助了重组蛋白在菌体内的正确折叠，提高了目的蛋白的可溶性。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。