蛋白的

With foaming degree of egg-white protein as response value, the optimum modification conditions for egg-white protein by xanthan were determined as follows: concentration of egg-white protein was 5.6%, addition amount of xanthan was 0.29%, the temperature was 45℃, the heating time was 22 min and the whipping time was 3.3 min.

以改性蛋清蛋白的发泡度作为响应值，对改性条件进行优化，最终确定了黄原胶对蛋清蛋白的最佳改性条件为：蛋清蛋白浓度为5.6%，黄原胶添加量为0.29%，加热温度为45℃，加热时间为22 min，搅打时间为3.3 min。

All Mut+and Muts colones were induced respectively with methanol to secrete interesting peptide in shake-flask cultures for four days. After dialysis and lyophilization, the supernate of cultures were identified by SDS-PAGE and Western blotting to find the genetically engineered Pichia pastoris which expressed interesting peptide.

在摇瓶中分别用甲醇诱导Mut~+和Mut~s型转化子表达蛋白4d，取培养产物冻干浓缩，进行SDS-PAGE和Western blotting，分析蛋白的含量及纯度，筛选出能特异表达目的蛋白的基因工程菌。

MyosinⅩ, a vertebrate-specific member of myosin super-family, can be detected in most tissues and cells, such as kidney, liver, brain, and so on. Full-length MyosinⅩcan stimulate the formation or stabilization of filopodia. As an actin-based motor protein, MyosinⅩplays a key role connecting cell membrane proteins and cytoskeleton.

肌球蛋白Ⅹ是脊椎动物所特有的细胞骨架蛋白，在脑、肝、肾等大多数组织中都有表达，它的显著特点是定位于细胞丝足的顶端和片状伪足的边缘，MyosinⅩ作为一个动力蛋白分子，起到连接细胞膜蛋白与细胞内骨架蛋白的桥梁作用，因此推测它可能在SVZa成神经细胞的迁移过程中起到重要作用。

Through optimization of thick mash fermentation conditions, suitable fermentation conditions for improving solubility of corn protein were determined, so as to improve the solubility of corn protein favorable for digestion of protein by animals, so that the utilization rate of the corn gluten meal as a feed could be increased.

通过甘浓醪发酵条件的优化研究，确定适宜的改善玉米蛋白溶解性的发酵条件，提高玉米蛋白的溶解性，有利于动物对蛋白的消化吸收，从而提高玉米蛋白作为饲料的利用率。

In addition to the two axial ligations provided by His18 and Met80, the hydrogen bond and hydrophobic interactions between the heme and the protein polypeptide, the heme also covalently linked to the protein matrix through two thioether bonds which are formed between vinyl groups of the heme and cysteine residues of a classic Cys-Xaa-Xaa-Cys-His heme-binding motif of the polypeptide chain.

Cyt c是血红素蛋白中电子传递蛋白的典型代表，其辅基heme与蛋白肽链的结合除了来自heme轴向配体组氨酸(His18)和甲硫氨酸(Met80)的配位作用、氢键和疏水相互作用等非共价键作用方式外，血红辅基heme的两个乙烯基还与蛋白肽链上的结构片段域Cys-Xaa-Xaa-Cys-His中的两个半胱氨酸形成硫醚键以共价键方式相结合。

The total contents of rice prolamine of 300 lines were determined with ultraviolet absorbing.

用醇溶蛋白提取剂提取了300个水稻品系的醇溶蛋白，紫外吸收法测定了水稻种子中醇溶蛋白的总含量。

A co-regulation existed in the expression among chalcone synthase, methionine sulfoxide reductase and acyl carrier protein Ⅱ, also did in the expression between allergenic protein and prolamine.

查尔酮合酶、S-腺苷基甲硫氨酸还原酶和酰基载体蛋白Ⅱ的表达存在基因间共调控，过敏反应蛋白和醇溶蛋白基因的表达也存在基因间共调控。

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明，6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异，表明HIV1的LTR及其下游基因置于痘病毒启动子控制下，在痘苗病毒中表达时有下述特点：（1）不同的痘苗病毒启动子与全长LTR相互作用，对gag基因表达有显著不同的调控效果；（2）NR序列对Gag蛋白表达没有明显影响；（3）EN序列不能被重组痘苗病毒表达系统识别；（4）TAR序列可提高Gag蛋白的表达量；（5）U5区及下游非翻译序列不影响Gag蛋白的表达。

After removing DNA, histones and part of NHPs, we found that actin and tropomyosin exist in the polytene chromosomal scafiold by immunofluorescence experiments.

对去除DNA、组蛋白和大部分非组蛋白的果蝇多线染色体的免疫荧光实验表明，肌动蛋白和原肌球蛋白存在于果蝇多线染色体骨架上。

It's regarded as a dentin specific protein, The gene structure and the expression pattern of DSPP at transcript level are well known in recent years, however, the expression pattern of DSPP protein, and it's possible role during tooth development and dental pulp reparation after injury are not clear yet.

近年来，DSPP的基因结构及mRNA水平的表达模式已较清楚。蛋白印迹实验证实小鼠牙胚中有DSPP蛋白的存在。但是，DSPP在蛋白水平的表达模式如何？它的生物学功能和作用机制是什么？它在牙髓损伤修复中的作用又如何？目前国内外均未见报道。这些问题的研究，对我们在该蛋白后基因水平的了解具有重要意义，也为我们深入了解牙本质生物矿化机制、对牙体组织疾病和损伤进行生物性防治提供理论依据。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。