蛋白的

The extraction procedure of crude protein from Vallisneria spiralis L. was to extract the crude protein with sodium hydroxide followed by precipitation with acid. Factors influencing the extraction rate were studied according to the experiments of odd factorsNaOH concentration,Vallisneria spiralis L.

采用碱提酸沉法从苦草中提取粗蛋白，通过单因子试验（单因素：NaOH溶液浓度、固液比、提取温度、提取时间）和L9（34）正交试验，对影响粗蛋白的提取工艺进行了研究；同时比较了不同pH值对粗蛋白沉淀的影响。

The molecular weights of the two subunits were shown 142 KD for the larger one and 47 KD for the smaller one, respectively, on SDS—polyacrylamid electrophoresis. The molecular weight of the native vitellin was determined as 205 KD on native gradient polyacrylamid gel electrophoresis.

用蒸馏水沉淀法从东方粘虫Mythimna seperata新鲜卵中提取卵黄蛋白，SDS-PAGE分析结果显示，粘虫的卵黄蛋白由大小两个亚基组成，大亚基分子量为142KD，小亚基分子量为47KD，线性聚丙烯酰胺梯度凝胶电泳结果显示，卵黄蛋白的天然分子量为204KD。

The PrP of bastard halibut consists of 472 amino acid residues, weighing about 49.6kD. The deduced amino acid sequence is 71.9% similar to that of Japanese pufferfish and 66.4% similar to that of Atlantic salmon, and bears the structural signatures of PrPs including a signal sequence, tandem repeats, a hydrophobic region, two glycosylation sites, a disulphide bridge and a glycosyl phosphatidylinositol anchor site.

推测的牙鲆蛋白质感染因子蛋白由472个氨基酸组成，分子量约49.6kD，具有信号肽、短肽顺接重复、疏水区、糖基化位点、二硫键、糖基缩醛磷肌醇锚定位点等蛋白质感染因子蛋白特征结构域，与红鳍东方鲀和大西洋鲑蛋白质感染因子蛋白的相似性分别为71.9%和66.4%。

Methods:Laser capture microdissection was used to separate human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells, Proteome alterations were observed using two dimensional polyacrylamide gel electrophoresis(2-DE) technique and electrospray ionisation tandem mass spectrometry.

通过激光捕获显微切割技术分离ESCC肿瘤细胞和癌旁上皮细胞，通过双向电泳和质谱技术鉴定表达异常的蛋白，并通过蛋白免疫印记证实部分差异蛋白的表达。

UPP can degrade intraeellular proteins with high effect and selectivity, especially for cell cycle regulated proteins, oncoproteins, tumor suppressor proteins, and denaturalized proteins.

当泛素-蛋白酶体系统对靶蛋白的降解功能失常时，可以引起癌蛋白聚集、抑癌蛋白异常降解、突变细胞凋亡受阻和增殖加速，从而导致肿瘤的发生。

Methods and materials A549 cells were collected and lysed for total protein after treated with metformin (5 mM) for 0, 15, 30 and 60 minutes. Western blot analysis was performed to detect the expressions of pan- and pho- AMPK, JNK and p38 proteins. Cleaved Caspase-3, 8, 9 proteins were also detected.

以5mmol/L的二甲双胍对A549细胞进行体外干预，在干预的第0、15、30和60min时，分别抽提细胞总蛋白，以Western blot技术检测细胞内AMPK、JNK、p38 MAPK的总蛋白和磷酸化蛋白的表达；在干预后第0、12、24和48h，检测剪切后的Caspase-3、8、9活性蛋白的表达水平。

The invention also encompasses nucleic acid molecules encoding the albumin fusion proteins of the invention, as well as vectors containing these nucleic acids, host cells transformed with these nucleic acids and vectors, and methods of making the albumin fusion proteins of the invention using these nucleic acids, vectors, and/or host cells.

本发明还包括编码本发明的白蛋白融合蛋白的核酸分子，以及含有这些核酸的载体，由这些核酸和载体转化的宿主细胞，和使用这些核酸，载体和/或宿主细胞制备本发明的白蛋白融合蛋白的方法。

The invention also encompasses nucleic acid molecules encoding the albumin fusion proteins of the invention, as well as vectors containing these nucleic acuds, host cells transformed with these nucleic acids and vectors, and methods of making the albumin fusion proteins of the invention using these nucleic acids, vectors, and/or host cells.

本发明还包括编码本发明的白蛋白融合蛋白的核酸分子，以及含有这些核酸的载体，被这些核酸和载体转化的宿主细胞，以及使用这些核酸、载体和/或宿主细胞制备本发明的白蛋白融合蛋白的方法。

After optimizing the expression conditions, a higher production could be obtained when GS115-proCPB was induced in BMGY(pH 6.0), at 28℃, with addition of 0.5% casein acid, 3 mmol/L PMSF, and 0.5% methanol per 12 h. The yield of recombinant protein reached 500 mg/L, and achieved over 94% of total protein in the culture supernatant.

通过表达条件的优化，使用BMGY(pH 6.0)培养基，添加0.5%的酪蛋白水解物和3 mmol/L的PMSF，于28 ℃培养，每隔12 h补加0.5%的甲醇，重组酵母GS115-proCPB 表达的重组蛋白可达500 mg/L 以上，表达的目的蛋白占总蛋白的94%以上。

There have been massive researches on improving the properties of protein in recent years, this article emphatically begin with the interaction between tea polyphenol and protein (egg albumen protein, soybean protein isolated, whey protein concentrated, bovine serum albumen), study their functional rules and that under the changed food processing environment (the pH value, salt ion concentration, sucrose density), and then apply the interaction rules into two different kinds of cakes for improving the technology of their production, aiming for provide the new mentality and method on improving the properties of protein as well as speed up the application of tea polyphenol.

本文着重从茶多酚与蛋白质的相互作用着手，研究了茶多酚及其主要单体组分对蛋清蛋白、大豆分离蛋白、乳清浓缩蛋白及牛血清蛋白的功能特性的影响规律，同时也探讨了环境因素(pH值、盐离子浓度、蔗糖浓度)与茶多酚协同作用对蛋白质这些功能特性的影响规律，并且初步应用了这种相互作用规律到两种不同工艺蛋糕的生产中来，目的是为蛋白质功能特性的改善提供新的思路和方法以及加快茶多酚在食品中的应用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。