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The composition and structure of vital wheat gluten,which contains the glutenin and gliadin subunits ,were reviewed in great detail in the paper.

详细介绍了小麦面筋蛋白中麦谷蛋白和醇溶蛋白的亚基组成和结构,以及各亚基与面团品质的关系。

The composition and structure of vital wheat gluten ,which contains the gluten in and gliadin subunits,were reviewed in great detail in the paper.

详细介绍了小麦面筋蛋白中麦谷蛋白和醇溶蛋白的亚基组成和结构,以及各亚基与面团品质的关系。

Compared with high-yielding cultivation in wheat, the SHY cultivation could significantly increase the capacity and contents of wet gluten, protein, albumin, prolamin and glutelin. There were no significances in globulin content and the ratio of glutelin to prolamin between the SHY and CK.

与普通高产栽培相比,超高产小麦栽培可以显著增加籽粒的容重、湿面筋含量、粗蛋白质含量、清蛋白、醇溶蛋白和谷蛋白的含量,对球蛋白含量与谷醇比无显著影响。

The results showed that human transferrin could be expressed in these cell lines and mouse or goat mammary gland following transfection, and its expression level could be improved dramatically when it was driven by rabbit enhancer and promotor as compared with goat beta lactoglobulin promotor. Transgenic mice were generated by either microinjection or lentivirus infection.

结果显示所有表达载体均可指导人转铁蛋白在细胞以及小鼠和山羊乳腺中表达,兔转铁蛋白增强子与启动子组合可显著性增加人转铁蛋白的表达水平,且其指导表达的效率明显高于山羊β乳球蛋白启动子。

It is prepared with phosphatide with excellent biocompatibility and cell compatibility as dispersant, protein and polysaccharide as medicine wrapping material and through co-agglomeration process with control proportion among protein, polysaccharide and bone morphogenetic protein.

它是利用具有良好的生物相容性与细胞相容性的磷脂类物质为分散剂,以蛋白、多糖为药物的包覆材料,通过控制蛋白、多糖的比例以及他们的混合体与骨形态发生蛋白的比例,采用共凝聚技术,获得粒径40~2000nm的水分散的骨形态发生蛋白注射制剂。

PIAS proteins play roles mainly through two mechanisms: acting as SUMO E3 ligases to promote modifications of some transcriptional factors and cofactors, especially sumoylation, and to regulate their transcriptional activation; acting as structural proteins to offer platforms for protein-protein interactions and to promote the abstraction and recruitment of other regulators in the cellular signal pathway complexes or gene transcriptional complexes, companied by the subnuclear location of target proteins.

PIAS蛋白的调控机制主要有两种:一种是通过其自身所具有的SUMO(small ubiquitin-related modifiers)E3连接酶活性,促进对一些转录因子、转录辅因子的化学修饰,尤其是SUMO化修饰,从而调控它们的转录活性;另一种是作为构架蛋白,为蛋白质之间的相互作用提供平台,促进细胞信号通路复合物或基因转录复合物中其它调节蛋白的去除和募集,并涉及到靶蛋白的亚核定位。

The central issue of this paper is the study of DNA-binding proteins interacted with DNA replication origin. The paper presented here includes there parts which are related as well as independent of each other:(1). Identification and partially purification of DNA-binding proteins interacted with eukaryotic DNA replication origin;(2) Specific binding of Hela cell proteins to Samian Virus 40 replication origin with the cruciform structure (Stem-Loop structure);(3) The study of DNA-binding protein as a tumor marker.

本文对DNA结合蛋白的研究主要包括三个即有关联,又相对独立的方面:(1)猿猴肾脏细胞复制起始区DNA结合蛋白的鉴定和部分纯化;(2)Hela细胞中DNA结合蛋白对含茎环结构的SV40复制起始区DNA的特异性结合;(3)DNA结合蛋白做为肿瘤征兆物的研究。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Cortis tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL/6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像;②获得了各种听功能密切相关的结构的成熟时间点,基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14);③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点:④Calbindin在E16以后的螺旋神经元没有表达,但是在scarpa神经元持续表达。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Corti\'s tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL/6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像;②获得了各种听功能密切相关的结构的成熟时间点,基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14);③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点:④Calbindin在E16以后的螺旋神经元没有表达,但是在scarpa神经元持续表达。

Some approved aneugens Trip terygiuwlIypogaucum (evelHutch, colchicine, thiabendazole, a tropine sulfate,d-ubocurarine was added into the reactive system respectively and the changes of their absorption values were taken down during different periods under the conditions of 37 0C and 350nm .

本研究通过多步聚合和解聚合反应从猪脑中分离纯化微管蛋白,建立微管蛋白的体外聚合和解聚合反应平衡体系,并将已证实的非整倍体诱发剂(昆明山海棠根部水抽提物、2-(4′-噻唑)苯丙咪唑、硫酸阿托品、d-筒箭毒碱)分别加入此反应平衡体系中,于37℃、350nm条件下,记录此体系不同时期的吸光值变化,与阳性对照物和溶剂对照处理平衡体系后的吸光值在相同时间点作比较,以此评估这几类非整倍体诱发剂对离体条件下的微管蛋白聚合的影响,寻求这几类非整倍体诱发剂是否可通过抑制微管蛋白聚合作用途径而诱发非整倍体。

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