蛋白的

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

Results as bellows: AtSIRT1 was located in Mitochondrial as hSIRT4 of human, and maybe take part in respiration and electron transformation chain, AtSIRT2 was located in nucleolus as hSIRT6 of human, maybe play important role in extend lifespan;mutation in AtSIRT1 leaded to cotyledon of plant turn to yellow and caused short life span. Mutation in AtSIRT2 could make the color of leaf turn to purple and accumulate a lot of anthocyanin;Sirtinol, a inhibitor of SIRT which did not cause the same model of the mutation of AtSIRT1 and AtSIRT2 indicated that the mechanism of Sirtinol was different from other organism;the structure of AtSIRT1 and AtSIRT2 were similar to other known Sir2, which indicated that they maybe have the same function;AtSIRT2 was overexpressed and its activity was detected.

结果表明，1，拟南芥AtSIRT1与人的同源蛋白hSIRT4相同，定位于线粒体，可能参与呼吸作用和电子传递，SIRT2与人的同源蛋白hSIRT6相同，定位于细胞核，可能同它的功能类似，在延缓衰老及调节细胞寿命方面起作用。2，AtSIRT1突变，可引起幼苗和植株的子叶变黄和早衰；AtSIRT2突变，可引起叶片发紫，沉积大量花青素。3，SIRT蛋白的抑制剂Sirtinol不能表型模写AtSIRT1和AtSIRT2突变体，说明Sirtinol在拟南芥中的作用机制不同于其他生物。4，AtSIRT1和AtSIRT2蛋白质结构预测表明与已知的Sir2蛋白相似，揭示其功能的相似性。5，在大肠杆菌中过量表达了其中一个基因(AtSIRT2)，可体外检测其酶学活性，进一步证明其功能。

The result showed that the temperature of denature for the formeris 106.67°C, while that for the latter is 66.84°C. These results showed that theoligosaccharide chain of SPWSG played a role of strengthening resistance todenaturation.

采用DSC对甘薯水溶性糖蛋白的热力学性质进行了研究，结果表明甘薯水溶性糖蛋白的热变性温度为106.67°C，而去糖基后的热变性温度为66.84°C，说明糖蛋白上的寡糖链对其有较好的稳定作用，具有增强糖蛋白抗变性的功能。

After induction and massive expression of the 3 fusion proteins, the soluble fraction was purified with 50% Ni-NTA resin affinity chromatography, which was proven by SDS-PAGE of the eluate to be a method that can achieve high purity of fusion proteins.

大量诱导表达三种融合蛋白后，取含FA、FB融合蛋白的可溶性组分用50％Ni-NTA树脂过柱纯化，将洗涤部分进行SDS-PAGE检测，表明用此纯化方法能纯化到高纯度的融合蛋白。

In vitro\% peritrophic membrane assay showed that all three truncated enhancin lost their mucin degrading ability, while a full length recombinant enhancin was active in the assay.

通过体外降解围食膜的方法检测这些部分缺失的增强蛋白的活性，结果证实这三种蛋白均失去了增强蛋白的降解围食膜粘蛋白的活性。

The composition and structure of Soybean Protein Isolate are first introduced,Then the functional modification of SPI via chemical ways which include the reaction of amino group,carboxyl group and sulphydryl group,via enzymic ways which include the modification using transglutaminase enzyme and papain,via physical ways which include blending and heat treatment are reviewed.

首先介绍了大豆分离蛋白的基本组成与结构，然后分别从化学改性、酶改性和物理改性三个方面对大豆分离蛋白改性进行了综述。其中，在化学改性方面，针对大豆分离蛋白中含有的氨基、羧基、巯基等不同活性基团的改性原理及研究现状进行了介绍。在酶改性方面，主要介绍了谷胺酰胺转胺酶、木瓜蛋白酶等对大豆分离蛋白的改性作用。在物理改性方面，介绍了共混、加热改性等目前研究较多的方法。

At present, the research mainly concentrates on vitellin purification and characterization, vitellogenin synthesis and hormonal regulation, vitellogenin absorption and processing.

目前，对蜱类卵黄发生的研究主要包括卵黄蛋白的纯化与鉴定，卵黄原蛋白的合成与调控，卵母细胞对卵黄原蛋白的摄取以及转变为卵黄蛋白的分子过程。

Using indirect ELISA, the contents of vitellin in the fat body, haemolymph and ovary of P. japonica were detected. The results suggested that the vitellogenin was synthesized in fat body at the 2nd day after eclosion. The contents of vitellogenin in fat body, haemolymph and ovary increased quickly on the 4th day after eclosion and reached the peak stage approximately on the 8th day in adult stage.

采用间接竞争ELISA法，系统测定了龟纹瓢虫成虫期脂肪体、血淋巴和卵巢中卵黄蛋白的动态变化，结果表明：脂肪体是卵黄原蛋白合成的场所，卵黄原蛋白的合成始于羽化后第2天；脂肪体、血淋巴和卵巢中卵黄原蛋白的滴度在羽化后第4天开始迅速上升，至成虫期的第8天左右达到高峰期。

As a kind of important raw material of protein,effects of high intensity pulsed electric fieldson the functional properties of egg albumen were studied.Results showed that solubility of egg albumen decrease when the pulsed electric fields intensity is more than 35 kV/cm.Emulsibility,foam capacity and hydrophobicity of egg albumen increase with the increase...

结果表明：蛋清蛋白的溶解度在脉冲电场强度大于35kV/cm时下降；蛋清蛋白的乳化性、起泡能力、泡沫稳定性及疏水性先随脉冲电场强度增加而增大，但当脉冲电场强度大于30kV/cm后，蛋清蛋白的乳化性、起泡能力、泡沫稳定性及疏水性下降；随着脉冲电场数的增加，蛋清蛋白的溶解度、乳化性、起泡能力、泡沫稳定性及疏水性变化不显著。

The results showed that under the cold stimulation, the types of blood protein both changeed, cold shock proteins was generated. Since the different genes which control CSPs need different intensity and degree of stimulation, the same specie of Loaches had the differences in the time and new protein generation under the different temperature treatment. Since two types of Loaches have different adaptability, the time and type of CSPs generation are also different under the same temperature treatment.

结果表明，泥鳅和大鳞副泥鳅的血液蛋白质发生了变化，即产生了冷激蛋白；同种泥鳅，控制产生冷激蛋白的特定基因在一定的刺激强度和刺激程度下，产生冷激蛋白的时间和种类不同；不同种泥鳅，在相同低温处理条件下，产生冷激蛋白的时间和种类也不一样。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。