蛋白核

It plays a key role in the process of cell division, cell movement, pollen tube elongation and so on.

ADF蛋白是一种在真核生物中广泛存在的低分子量的肌动蛋白结合蛋白，在细胞分裂、细胞运动、花粉管伸长等重要的生命活动中发挥着重要的作用。

With brain stereotaxis technique PP2A was inhibited in the nucleus basalis of Meynert and the cytoskeletal protein tau, neurofilament were found hyperphosphorylated and aggregating. The neurite became short and thickening, acetyltransferase aggregated in the body of neuron and the acetylcholine level in the nucleus basalis of Meynert was significantly decreased followed with spatial memory defict of rats.

利用立体定位技术抑制大鼠基底核PP2A活性，使基底核神经元微管相关蛋白tau及神经细丝出现过度磷酸化和聚积，轴突变短增粗，胆碱乙酰转移酶在胞体聚集，而使基底核乙酰胆碱含量降低，大鼠出现空间记忆障碍。

But the latent period for reaching the platform above the water in Morris Water Maze of the Mn-exposed group was not different from that of control group. There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高；(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高；(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

E. , ACTH antagonizes the analgesia mediated byμand δ opioid receptors, but notκreceptor;(2) The antagonizing effect of ACTH on opioid analgesia is proposed to be mediated by ACTH receptors, although the latter has not been characterized;(3) A contradictory interaction on intracellular cAMP content may constitute one of the postreceptor mechanisms underlying ACTH antagonism of opioid analgesia;(4) Another proposed mechanism of the anti-opioid effect of ACTH is that ACTH can modulate opioid-induced suppression of calcium influx;(5) ACTH has been shown to induce Fos protein expression in selected areas of the rat brain including the nuclei involved in pain regulation as well as the ependyma of the third ventricle and the aqueduct.

根据以上实验结果，本论文首次提出以下论点：（1）ACTH在脊髓水平对抗阿片镇痛具有受体选择性，即ACTH可对抗μ受体和δ受体介导的镇痛，不对抗κ受体所介导的镇痛；（2）由于ACTH与阿片μ受体的肽类配体的结合位点仅有很低的亲和力，与μ受体的非肽类配体的结合位点以及δ受体无亲和力，推测ACTH是通过中枢内的ACTH受体介导发挥抗阿片效应的；（3）ACTH抗阿片作用的受体后作用机制之一是在cAMP信使通路水平与阿片发生相互作用；（4）ACTH抗阿片作用的另一受体后机制是在〓水平影响阿片的效应；（5）通过Fos蛋白的诱导揭示：ACTH可以作用于脑内多个核团，其中包括许多与痛觉调制有关的核团，推测ACTH可能通过激活这些核团的神经元而发挥其中枢效应。

With the aid of PDQuest 2-DE software, 11 significant protein dots were found out and then 9 of them are pick out to go through peptide mass fingerprinting based on MALDI TOF MS and NCBI database searching . In all, 5 proteins were identified: Ribulose bisphosphate carboxylase , Nucleic acid-binding protein , Ribosomal protection tetracycline resistance protein , hypothetical protein FG04726.1 [Gibberella zeae PH-1], nitric oxide synthase .

应用PMF技术对这11个具有显著表达差异蛋白点中的9个进行鉴定，得到了5个质谱阳性鉴定结果，其中2个蛋白分别为玉米种属的1，5-二磷酸核酮糖羧化酶／加氧酶和叶绿体的核酸结合蛋白；其它的3个蛋白在玉米蛋白质数据库中未见报道，但与其它物种基因编码的蛋白质具有高度的序列同源性。

The new method has better resolution and can be applied to larger systems than homonuclear NMR methods. Most proteins playing an important role in cells have to be studied with heteronuclear labeled samples.

异核方法的优势在于它的分辨率更好，可以研究相对较大的，同核方法研究不了的分子；对大部分在细胞中发现的意义重大的蛋白的NMR研究都必须采用异核方法。

Genomic DNA in eukaryotic cell is organized into nucleosome array and further packaged into higher-order structures. Each nucleosome is composed of a stretch of approximately 147bp DNA wrapping around a histone octamer, and neighbored nucleosomes are connected by linker DNA with length varies from 20bp to 100 bp.

中文摘要真核生物的基因组DNA能够与组蛋白形成核小体结构，这些核小体结构在基因组上线形排列，并进一步折叠成更加紧密和复杂的高级结构。

The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.

分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme，HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme，HdvRz)，并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游，构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT，经Plasmid Midi Kit纯化后，在Lipofectamine~ 2000的介导下，直接转染DFI细胞，72h后取上清继续在CEF上传代。

Protein phosphatases are crucial for cell living. Protein phosphatase 2A (PP2A) is an important member of serine/theronine protein phosphatase family, which is involved in almost all biological processes in eukaryotic cell. The 3D structure of PP2A core enzyme and holoenzyme was solved in 2006. These results are significant instructions for us to further understand PP2A structure, interactions between PP2A subunits, and also interactions between PP2A and its binding proteins.

蛋白磷酸酶在细胞的生命活动中起着十分重要的作用，蛋白磷酸酶2A(protein phosphatase 2A， PP2A)作为蛋白磷酸酶家族中十分重要的一员，它几乎与所有真核细胞的生命活动都有密不可分的关系。2006年，PP2A核心酶和全酶晶体结构的陆续破解对于深入了解PP2A自身的结构和亚基之间的相互作用，以及其与结合蛋白作用的机制都有重大的影响。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐