蛋白核

When Tetrahymena, Euglena, Crypthecodinium and Oxyrrhis were stained with the same technique, although all the nuclei gave positive reactions, no precentromeres could be distinguished; within nuclei there were only patches composed of minute fluorescent spots seen.

在对小眼虫和寇氏隐甲藻作制备核骨架的细胞分级抽提处理后，细胞核仍还都呈阳性反应。这表明与高等真核生物的相似，低等真核生物的着丝粒蛋白也是与核骨架紧密结合的。

PIAS proteins play roles mainly through two mechanisms: acting as SUMO E3 ligases to promote modifications of some transcriptional factors and cofactors, especially sumoylation, and to regulate their transcriptional activation; acting as structural proteins to offer platforms for protein-protein interactions and to promote the abstraction and recruitment of other regulators in the cellular signal pathway complexes or gene transcriptional complexes, companied by the subnuclear location of target proteins.

PIAS蛋白的调控机制主要有两种：一种是通过其自身所具有的SUMO(small ubiquitin-related modifiers)E3连接酶活性，促进对一些转录因子、转录辅因子的化学修饰，尤其是SUMO化修饰，从而调控它们的转录活性；另一种是作为构架蛋白，为蛋白质之间的相互作用提供平台，促进细胞信号通路复合物或基因转录复合物中其它调节蛋白的去除和募集，并涉及到靶蛋白的亚核定位。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

In this project,by using the primitive eukaryotic cell Giardia lamblia as materials,the relationship between lamina and nuclear division, and the origin and evolution of lamin family and were studied.

本课题旨在从起源进化的角度出发，以相对简单和原始的真核生物贾弟虫为材料，探讨核纤层在该原始细胞中的成分和基因，及其与核分裂的关系，还有核纤层蛋白家族的起源进化规律。

The histone octamer may protect the wrapped DNA from interacting with other environmental factors such as transcription regulating proteins, thus the positioning of nucleosomes along DNA may play important roles in multiple cellular processes including DNA replication, transcription and damage-repair.

由于核小体结构中的DNA缠绕在组蛋白八聚体上，可能影响这些DNA与细胞内的其他蛋白相互作用，从而影响到DNA复制、转录等生物学过程，因此，核小体在基因组上的位置分布可能对基因组功能具有重要的调控作用。

To understand the listed contents:the studies about component of nuclear pore complex; the main functions of gp210;the different configuration models of sequence specific DNA-binding proteins; karyotype and chromosome bandings; configuration of chromatin and dene transcription; nuclear bodies is many subnuclear domains which have different conformation between chromatin.

了解核孔复合体成分的研究；gp210主要的功能；序列特异性DNA结合蛋白的不同结构模式；核型与染色体显带；染色质结构与基因转录；染色质之间的空间发现的许多形态上不同的亚核结构域——核体。

The eukaryotic translation initiation factor 5A (eIF-5A) is a small acidic protein (18k Da), widely presents in all organisms from archebacteria to mammals but not in eubacteria, with highly conserved sequences. eIF5A is unique in that it is the only protein known to contain the unusual amino acid residue hypusine (hydroxyputrescine-lysine), which is essential for eIF5A function and is formed by post-translational modification of a specific lysine residue.

真核生物翻译起始因子5A(eIF5A)是一个分子量约18 kDa的酸性蛋白，其序列从古细菌到哺乳动物高度保守，并广泛存在于各种真核生物细胞中。eIF5A是迄今为止唯一一种已知含有特殊氨基酸残基hypusine的蛋白质。hypusine是一种蛋白翻译后修饰产物，它的形成对eIF5A的功能完整至关重要，相关的研究结果表明hypsine在控制细胞周期循环的过程当中扮演着一个重要的角色。

Results］ There was significant relationship of the hMSH2 protein expression levels to location in gastric cancers, the expression in nucleus or in nucleus and plasm was stronger than that only in plasms, but both of the expression level and location were not related to the differentiation of the cancers.

结果］胃癌hMSH2蛋白表达强度及表达部位均与胃癌分化程度无显著相关关系（P＞0.05）； hMSH2蛋白表达强度在核表达及核、浆同时表达者明显强于浆表达者（P＜0.05）。

The genome of wheat rosette stunt virus, a plant rhabdovirus, is a single negative strand RNA.

小麦丛矮病毒是在中国发现的一种植物弹状病毒，病毒基因组是由一条单链负链RNA组成并编码 5种病毒结构蛋白质：表面糖蛋白G、膜基质蛋白M、核衣壳蛋白N、大蛋白L和所谓非结构蛋白NS。

These include nuclear lamina proteins,mRNA processing-related proteins,cell cycle-related proteins and synthesis-related enzymes,all of which were involved in basal metabolism and biological process in cells.

这些蛋白包括核纤层蛋白、mRNA表达调控蛋白、细胞周期相关蛋白和生物大分子合成相关蛋白等，功能涉及细胞基本代谢和生理过程的多个方面。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐