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RESULTS: The activity of heparanase in the mammalian cell lysate detected was much higher than that in the culture medium. The molecular mass of the heparanase protein expressed in the transfected COS7 and CHO cells was about 53×103 by western blot. CONCLUSION: The fulllength heparanase gene is isolated.

结果:分离扩增出1629 bp的人HPA全长cDNA序列并构建了其真核表达载体;在转染后的COS7细胞和筛选出的CHO细胞裂解液中检测到较高的HPA活性和相对分子质量大小约53×103的目的蛋白免疫印迹表达带。

METHODS: Fulllength or fractions of VP22 were fused to C terminal of HBV core protein, and cloned into pcDNA3.1 vector, yielding eukaryotic expression plasmids of DN mutant.

将VP22全长及其不同区段融合于HBV核心蛋白的C端,克隆入pcDNA3.1构成DN突变体真核表达质粒。

Objective To construct the eukaryotic expression vector for pathogenic gene MYOC of primary open angle glaucoma and express in COS-7 cells.

目的 构建原发性开角型青光眼致病基因MYOC的真核表达质粒,并在COS-7细胞中表达MYOC蛋白。

The early alteration was that of structure and function of mitochondrion and the ex...

早期表现为线粒体结构及功能的改变并有抗凋亡蛋白 bcl- 2的表达,后期电镜下可见凋亡特征性核结构改变及典型的 DNA&阶梯状&电泳带。

To explore the effect of pravastatin on production of oxLDL-inducd human peripheral blood monocytes.

为探讨普伐他汀对氧化修饰低密度脂蛋白诱导的人外周血单个核细胞分泌功能的影响。

Methods Primary brown adipocytes from rat fetuses, and from wild-type neonate mice and neonate mice deficient in the gene encoding protein tyrosine phosphatase-1B (Ptpn1, also known as Ptp1b) were cultured in the absence or presence of TNFα and different nuclear receptor agonists.

原代棕色脂肪细胞自大鼠胚胎、野生型新生小鼠和蛋白酪氨酸磷酸酶-1B基因基因(Ptpn1,也被称作 Ptp1b)缺陷新生小鼠,在加或者不加TNFα和不同浓度核受体激动剂中培养。

Present address: Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany and Department of Gastroenterology, Hepatology and Endocrinology, Medical School Hannover, 30625 Hannover, Germany

其中, XPO4基因编码核输出蛋白,是EIF5A2的作用底物,该基因在肿瘤细胞中被过度表达,它是 XPO4缺陷的肿瘤细胞增殖必须的因子,可促进肝癌细胞生长。

Objective To investigate the expression and clinical significance of NFκB family proteins in ovarian tumor.

目的探讨核转录因子家族蛋白在卵巢癌组织中的表达及其临床意义。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。