蛋白核

In eucaryotic cells, green fluorescence fusion vector was constructed which showed the localization of this protein in cytoplasm.

在真核细胞中，构建了绿荧光融合载体，证明了此蛋白在细胞质中的定位。

MAIN OUTCOME MEASURES: Construction and purification of Znf430 prokaryotic expression vector as well as its location in the cytoplasm of eucaryotic cells.

主要观察指标：锌指蛋白 403的原核表达载体的构建、纯化及在真核细胞细胞质中定位的结果。

OBJECTIVE: To construct a zinc finger protein 403 (Znf403) prokaryotic vector and observe its expression in eucaryotic cells.

目的：构建锌指蛋白403基因原核表达载体，观察其在真核细胞的表达情况。

Actin, as a component of the complex skeleton in the eucaryotic cell, takes part on many important cellular functional progresses.

肌动蛋白是真核细胞骨架的主要成分，与细胞内的许多重要功能活动有关。

RESULTS: All the four recombinant eucaryotic plasmids were constructed successfully, and HBV core protein expression was confirmed by internal reference for transfection.

结果： 4份重组真核表达质粒均构建成功，转染至HepG2后通过内参照EGFP可鉴定出均有HBV-C蛋白表达。

Objective To explore the subcellular localization of HBVDNAPTP1 transactivated by hepatitis B virus DNA polymerase with green fluorescent protein expression system in eucaryotic cells, and predict the protein structure by bioinformatics.

目的 利用真核细胞绿色荧光表达系统分析乙型肝炎病毒DNA聚合酶反式调节蛋白HBVDNAPTP1的亚细胞定位，并用生物信息学方法对其结构进行分析。

The PEA gene product was detected in the medium with Western blot, which indicated that the PEA gene with original signal peptide could be expressed with secretary form in eucaryotic cells.

表达产物经蛋白免疫印迹法（Western-blot）检测，表明本文构建的含有原基因信号肽的PEA基因能够在真核细胞进行分泌性表达，这为基于PEA的免疫毒素、疫苗佐剂和疫苗载体的研究奠定了基础。

In vivo footprinting is a method of studying DNA-protein interaction. It can reflect the authentic status of DNA-protein in vivo, and can also detect the change of DNA conformation. The introduction of Ligation-Mediated PCR greatly improves the sensitivity and specificity of in vivo footprinting study, and has facilitated the executation of in vivo footprinting in the regulation of eucaryotic gene expression.

体内足迹法是一种研究DNA-蛋白质相互作用的方法，它能真实反映体内蛋白-DNA相互作用的情况，而且可以检测体内的DNA构象的变化，连接介导的聚合酶链反应(Ligation-Mediatedpolymerase chain Reaction，LM-PCR)的发明，使得体内足迹研究的敏感性和特异性大大提高，有力地促进了体内足迹技术在真核基因表达调控研究方面的应用。

In the medical field, using genetic engineering has successfully expressed many kinds of cytokines in protokaryon Bacillus coli, eukaryon yeast system and mammalian cells, which will make possibility of large-scale production of recombinant cytokines.

在医学领域，应用基因工程技术已在原核大肠杆菌、真核酵母和哺乳动物细胞成功表达了多种细胞因子，使重组细胞因子蛋白的大量生产和应用成为可能。

The pIRES-EGFP-HCN2 eukaryon expression vector is successfully constructed, with double activity of HCN2 and EGFP. IRES-induced exogenous gene HCN2 can express in human embryonic kidney cells.

实验成功地构建了pIRES-EGFP-HCN2真核共表达载体，具有HCN2、增强型绿色荧光蛋白的双重活性，IRES能够介导外源基因HCN2在人胚胎肾细胞表达。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐