蛋白核

Homologous analysis of the deduced amino acid sequences were performed by BLAST and subsequently compared with GenBank data.

根据衣藻、矮牵牛花、Pisumsativum等真核生物hsp70a氨基酸高度保守序列设计两对简并引物，以热休克盐藻cDNA为模板进行巢式PCR扩增，产物经T-A克隆转化至JM109大肠杆菌中，经筛选后测序，将测序结果推导成氨基酸序列进行同源性分析，获得了盐藻热休克蛋白70a cDNA片段。

Results The molecular relative mass of immunocytokine, IP10-scFv is 41.1 kD, affinity constant of IP10-scFv is 2.28×10^-8 with Scatchard formula.

结果重组免疫细胞因子（IP10-scFv）真核表达产物的相对分子质量约为41.1 10^3，纯化后的蛋白浓度为68mg／L，抗体亲和常数（KDIP10-scFv）为2.28 10^-8mol／L。

Three hours following seizures there was an evident expression of c-fos proteins in the hippocampus (CA1 area), dentate gyrus, piriform cortex, dorsal part of entorhinal cortex, and amygdaloid nucleus, and there was a dramatic increase of c-fos proteins in CA3 area and the areas mentioned above except the CA1 area where c-fos proteins apparently decreased after electroacupuncture treatment.

在海马青霉素致痫模型上观察了致痫及致痫加电针后脑内c-fos蛋白的变化，结果表明致痫时海马〓区、齿状回、梨状皮层、背侧内嗅皮层、杏仁核见到较多的c-fos表达；电针后c-fos在〓区明显减少，在〓区及上述其余各区则显著增加。

Objective To explore the peripheral blood albumin gene marker in clinical value of diagnosis and monitoring metastasis of hepatocellular carcinoma.

目的探讨外周血有核细胞中白蛋白基因在肝癌诊断和转移监测中的价值。

Two kinds of DALF, DALFA and DALFB, was isolated in the DEAE-cellulose column using process elution method, SDS-PAGE analysis revealed the difference between the two eluates was the different ratio of H:L of the twenty four subunits.

通过离子交换层析柱(DEAE-52)分步盐洗脱技术，分离到两种不同分子量的蛋白质组分(DALF_A和DALF_B)，通过SDS凝胶电泳分析证明DALF_A和DALF_B不同之处在于蛋白壳的H和L亚基之间结合比例不同，由此形成释放铁动力学特性和铁核中的磷铁组成比也存在一定差异。

Results During the committed differentiation, the expression of calmodulin was distributed in and around the cell nucleus.

结果在神经干细胞定向分化为神经元的过程中，钙调蛋白于细胞核及核周呈阳性表达，随分化神经元的生长细胞核阳性表达逐渐减弱而胞浆增强，同时可见阳性反应物伸入树突及轴突。

Objective To explore the role of nuclear factor-κB in the signal conduction of protein kinase C regulated proliferation, apoptosis and expression o f Th2 cytokines - interleukin-4 (IL-4) and interleukin-5 (IL-5) of T lympho cytes in the bronchial alveolus lavage fluid.

目的探讨核因子-κB在蛋白激酶C对豚鼠哮喘模型T淋巴细胞功能调控的信号传导中的作用。

Results:The positive expression of P53 and c－Myc protein in nuclei was bound up with poor-differentiated tumours;the positive expression of Fas and c－Myc protein in cytoplasms was consistent with well-differentiated tumours.

采用免疫组织化学方法对骨肉瘤、骨化性纤维瘤、骨纤维结构不良和正常骨痂组织进行c－Myc、Fas和P53蛋白测定，与肿瘤组织学分级、细胞分化及核丝分裂比较分析。

MUC3 apomucin and its mRNA were mainly situated in the cytoplasms of goblet cells and columnar-type cells of duodenum and column.But no reactive signal of MUC2 and MUC3 genes' product was found in the mucous droplets.

MUC3核粘蛋白及其mRNA主要存在十二指肠和结肠的杯状细胞和柱状细胞胞浆内，而杯状细胞的粘液滴内无MUC2、MUC3基因产物的阳性反应信号。

PART ONE CONSTRUCTION OF VECTOR CARRYING HUMAN INSULIN-LIKE GROWTH FACTOR-1 GENE AND THE GENE EXPRESSION IN EUCARYOTE Insulin-like growth factor-1(IGF-1) is one of the most important regulate factors which can stimulate cartilage cell to differentiate and synthesis collagen typeⅡand proteoglycan to maintain cartilage phaenotype.

第一部分：携带hIGF-1基因重组载体的构建及在真核细胞的表达胰岛素样生长因子-1(IGF-1)是在软骨发育和软骨自稳态调节中最重要的生长因子之一，能刺激软骨细胞分裂增殖，并促进软骨细胞合成Ⅱ型胶原和蛋白聚糖，维持软骨细胞的表型等。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐