蛋白核

ARC1(arm repeat containing 1 ) is a very important downstream protein factor in sporophytic self- incompatible signaling process. It is made up of arm repeat domain, U-box domain, leucine zipper domain/ coiled-coil domain, one nuclear localization signal and two nuclear export signal, arm repeat domain and U-box domain are main functional domain.

臂重复蛋白ARC1(arm repeat containing1，ARC1)是孢子体型自交不亲和信号传导途径下游非常重要的蛋白质因子，由臂重复结构域、U-box结构域、亮氨酸拉链/卷曲螺旋结构域、1个核定位信号和2个核输出信号组成，其中臂重复结构域和U-box结构域起主要功能。

Anyway, we proposed a more detailed structure model of this kind of ATPases using staphylococcal CadA as an example.

总而言之，以葡萄球菌上的CadA当一个例子，我们提供了CPx型腺核甘三磷酸酵素这类蛋白更完整的结构模型。

Results MUC5AC apomucin was extensivoly distributed in the 1/3 superjacent gastric mucosa (100%).

结果 人正常胃粘膜中浅表1/3范围内广泛分布MUC-5Ac核粘蛋白(100％)。

MUC5AC apomucin was detected by immunohistochemical assay. Results: MUC5AC apomucin was distributed in the 1/3 superjacent gastric mucosa (100％).

应用免疫组织化学SP法检测人正常胃粘膜、癌前病变粘膜以及胃癌组织中MUC5AC核粘蛋白的表达。

PlasmaLDL was decreased markedly in cholesterol gallstone than in control group (p0.05). In cholesterol gallstone group, plasma TG and TG/TC ratio were significantly positive correlativity. Conclusions: These results indicate that the changes of plasma lipids may affect the metabolism of cholesterol and bile acids and itmay tade an important role in the formation of cholesterol gallstone. TG/TC ratio may act as an indirect reflect of reference index.

本实验结果提示：血浆脂蛋白的变化，可能因影响体内的胆固醇及胆汁酸等代谢而与胆囊胆固醇结石的形成密切相关，TC、TG的测定及TG/TC比值可以作为间接反映胆囊胆固醇结石成核的参考指标。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

SUMO has been shown to covalently modify a large number of proteins, reversible modification by SUMO regulates nucleocytoplasmic translocalization, protein-DNA binding activity, protein-protein interaction, transcriptional regulation, DNA repair, and genome organization.

SUMO与靶蛋白之间这种可逆的共价连接，在核质运输、DNA 与蛋白质结合活性、蛋白质之间相互作用、转录调控、DNA 修复以及维持基因组稳定等方面均发挥着重要的调节作用。

Heat shock factor binding protein 1(HSBP1) is a nuclear-localized, novel, conserved, low molecular weight (00 residues) transcriptional factor, which may repress the activity of the heat shock factor 1 (HSF1) by binding HSF1 active trimerization domain.

热激因子结合蛋白1（heat shock factor binding protein 1， HSBP1）是一种新发现的高保守、低分子质量、定位于核中的一种转录因子，它可抑制HSF1的转录域活性，并协同HSP70负调控热激反应。

HTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start site.

人端粒酶逆转录酶是一种核糖核蛋白酶，由端粒RNA和端粒酶蛋白组成的一种特殊逆转录酶，在激活端粒酶的过程中起限速作用，能以自身RNA为模板合成端粒序列以稳定端粒长度使细胞获得永生，也是人类端粒酶的活性中心，其表达是端粒酶活化的必须前题［2～4］。

Morphology of the EPCs was observed, and the growth curve of the EPCs was investigated. Surface antigens of the EPCs were analyzed by the flow-cytometer. The capability of intaking the acetylated low-density lipoprotein of the EPCs was detected using fluoresencent chemical method. The vasoformative capability and genetic stability of EPCs were cultured in matrigel, and examined by karyotype analysis.

观察培养细胞的形态，测定其生长动力学，采用流式细胞仪及免疫细胞化学方法检测其表型，应用细胞荧光化学法分析EPCs对乙酰化低密度脂蛋白(acetylated low-density lipoprotein， ac-LDL)的摄取功能，Matrigel上培养并检测其形成血管能力，染色体核型分析其遗传稳定性，裸鼠实验、软琼脂实验观察致瘤性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐