蛋白核

The immunocytochemistry was used to identify the RASMC.RESULTS: The RASMC were successfully cultured by the adherent method of tissue explants and grown in the "peak- valley" mode. The cultured RASMC were fusiform shape with a big oviform or round nuclei rich in cytoplasm. Immunohistochemical method ( S-P method) showed strong expression of a- smooth muscle actin.

结果：组织块贴壁法成功培养出SD大鼠胸主动脉平滑肌细胞，培养的细胞呈典型&峰-谷&样生长，HE染色细胞呈梭形，胞浆丰富，核大而圆或椭圆，免疫组化S-P法检测a-平滑肌肌动蛋白单克隆抗体呈强阳性表达。

IL-6 might participate the patho-physiology process of EH.; Up-regulating expression of IL-6 mRNA may be one of the mechanisms increasing protein IL-6 in peripherial blood of EH patient.

IL-6可能参与了原发性高血压的病理生理过程，外周血单个核细胞的IL-6 mRNA表达上调可能是原发性高血压患者外周血IL-6蛋白水平升高的机制之一。

Objective: To investigate specific antitumor immune responses induced by autologous peripherial blood mononuclear cells from patient with gastric cancer pulsed with antigen GST-MAGE-3 in vitro.

目的：体外观察GST-MAGE-3蛋白刺激胃癌患者外周血单个核细胞，诱导特异性抗肿瘤免疫应答。

Peripherial blood mononuclear cells pulsed with GST-MAGE-3 were able to present antigen to T lymphocytes, activate antigen-specific CTLs and induce special antitumor immune responses in vitro.

经GST-MAGE-3蛋白抗原冲击的胃癌患者外周血单个核细胞能在体外有效提呈抗原，激活抗原特异性CTL，诱导特异性抗肿瘤免疫应答。

Anthraea pernyi nucleopolyhedrovirus ; Polyhedral envelope protein gene ; Sequence of amino acid

柞蚕核型多角体病毒；多角体膜蛋白基因；氨基酸序列

The intracellular expression of viral gene and phenotypical change of transformants were examined.

结果：分离获得了可长期体外培养的SV40转化细胞克隆，已传23代以上，体外存活超过250d；DNA印迹实验显示SV40大T基因已整合至细胞染色体内，间接免疫荧光检测示多数细胞胞核内有大T基因产物的表达；转化细胞对裸鼠无致瘤性，并可正常表达角蛋白。

Objective To investigate the expression and significance of Ki67,cytotoxic proteinsTIAI,GranzymeB in nasal lymphoma.Methods Paraffin-embedded tissues of34cases of nasal NK/T-cell lymˉphoma,8midline B-cell lymphomawere studied using immunohistochemical method.

目的 探讨核抗原Ki67和细胞毒蛋白TIAI、GranzymeB在鼻腔淋巴瘤的表达及意义方法以NK/T淋巴瘤34例石蜡组织标本研究对象，同部位B细胞淋巴瘤8例为对照，行免疫组织化学染色。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

Part I. Molecular Characterization of HIV-1 genome by using fission yeast as a model systemAs a fungus, S. pombe has many features mimicking mammalian cells. Most of proteins have same functions in S.pombe cells and mammalian cells.

第一部分以S.pombe为模型对HIV-1全基因组的研究 S.pombe是与哺乳动物细胞非常相似的一种真菌，各种真核细胞蛋白在S.pombe细胞和哺乳动物细胞中的功能基本相似。

He volume of cerebral infarction was calculated by TTC staining, the expressions of NF-κB、Bcl-2 and Bax proteins in procerebrum cortices and basal nuclei of rats were detected by immunohistochemistry.

TC染色法测量脑梗死体积，免疫组织化学方法检测前脑皮质、基底核NF-κB、Bcl-2和Bax蛋白的表达。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐