蛋白核

Results There existed decreasing learning and memory function, increasing MAO-B activity from the cortex, hippocampus, and strium, elevating brain iron and aluminum levels with diminishing ferritin level, and karyopyknosis as well as neuron loss of hippocampus in the model mice.

结果 模型组小鼠学习记忆能力明显下降，大脑皮层、海马和纹状体MAO-B活力增加，脑铁水平明显升高，线粒体铁蛋白水平下降，而海马CA1区神经元核固缩和神经细胞丢失。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果：(1)通过细胞培养和MTT法，表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用，加药组与对照组相比其生长抑制率有显著性差异(P＜0.01)，而且这种抑制作用呈现浓度和时间的依赖性；(2)通过集落形成率的测定结果表明，加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P＜0.01)，说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用；(3)光镜观察结果表明，加药组可见细胞脱水浓缩伊红染色增强，胞体缩小，内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态；经AO／EB荧光染色观察结果表明，当终浓度为1.0mg／ml的姬松茸提取物作用于MGC-803胃癌细胞24h后，其凋亡率和破膜率与自然凋亡率与破膜率相比，均有显著性差异，其凋亡率86.3％(P＜0.001)，破膜率为41.6％(P＜0.01)，说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用，同时也有细胞毒作用，但以诱导细胞凋亡为主；(4)免疫组化结果表明，用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P＜0.001)。

These results suggest that keratin filaments may play an important role in nuclear assembly in Xenopus egg extracts which is not reported before.

这些结果显示角蛋白纤维在核膜重新形成的过程中起到重要的作用。

Over expressive mutant lamin A is toxic to the development of zygotes, Only zygotes with no target gene integration could survive until to be born.

过量表达的突变核纤层蛋白A对小鼠受精卵有致死性，只有无目的基因整合的受精卵才能存活至出生。

Although nuclear cascades in ethylene signal transduction pathway from EIN3 to ERF1 to PR proteins has been established in Arabidopsis thaliana, a similar route from EIN3 to EREBPs to fruit ripening and senescence has not been elucidated in Lycopersicon esculentum .

乙烯对植物的生长发育起重要的调控作用，随着乙烯信号转导研究的不断深入，许多乙烯信号转导组分已被分离和鉴定，并在模式植物拟南芥中已建立乙烯信号传递过程中的核内级联反应：EIN3→ERF1→病原相关蛋白，但在番茄中还没有建立EIN3→EREBPs→果实成熟相关基因的相似路径。

A pair of primers producing 614bp amplification fragment were designed based on the sequence of the C\|polyhedrin gene of Bombyx mori CPV,and the expected amplification products were obatined when genomic dsRNAs isolated from purified BmCPV,DsCPV and Lymantria dispar CPV were used as templates.Genomic dsRNAs isolated from Heliothis armigera CPV and DNAs from Lymantria dispar nuclear polyhedrosis virus and DNAs from midgut tissue of healthy Dendrolimus spectabilis larva did not yield any amplification products.The detection limit of purified DsCPV genomic dsRNAs was 1.0pg.

依据家蚕质型多角体病毒质型多角体蛋白的核苷酸序列设计一对引物，从纯化的DsCPV\，BmCPV和舞毒蛾质型多角体病毒的基因组dsRNA可成功地扩增出长614bp的目的片段，从提取的健康松毛虫幼虫肠组织的DNA、舞毒蛾核型多角体病毒基因组核酸、以及棉铃虫质型多角体病毒基因组核酸，未能扩增出目的片段。

Immunofluorescence histochemistry combined with retrograde tracing technique was employed to observe the effects of masseteric nerve transection on the expression of Trk ( tropomyosin-related kinase) receptor proteins, namely TrkA, TrkB and TrkC in the trigeminal mesencephalic nucleus (Me5) of the rat.

本研究应用免疫荧光结合逆行束路追踪技术观察了轴突切断对支配咬肌的三叉神经中脑核(Me5)神经元所含Trk受体蛋白，即TrkA、TrkB和TrkC表达的影响。

Refers to Down's syndrome (Down's syndrome, dementia-like伸舌) screening, that is, pumping blood or amniotic fluid do villus cells, and then do Karyotype analysis, such as the emergence of 21 - trisomy map, you can diagnose fetus for Down syndrome. 2, AFP Chinese name for the AFP, such as maternal and fetal amniotic fluid were increased AFP confirmed fetal neural tube defects, namely spina bifida, spinal Meningocele, no brains infants, microcephaly, hydrocephalus.

是指唐氏综合症的筛查，就是抽绒毛血或羊水做细胞培养，然后做染色体核型分析，如出现21-三体综合征图谱，即可诊断胎儿为唐氏综合征。2、AFP中文名称为甲胎蛋白，如母血及胎儿羊水AFP均增高，证实胎儿有神经管畸形，即脊柱裂、脊脑膜膨出、无脑儿、小头畸形、脑积水。

Methods:Immunohistochemical technique was used to detect the p21 WAF1/CIP1and P53 protein expressions in:20 usual leiomyoma,70 borderline leiomyoma:including 40 cellular leiomyoma,13 bizarre leiomyoma,4 mitotically active leiomyoma,10 atypical leiomyoma, 3 smooth muscle tumors of uncertain malignant potential and 18 leiomyosarcoma...

应用免疫组织化学法检测 2 0例普通型子宫平滑肌瘤，18例子宫平滑肌肉瘤及 70例交界性子宫平滑肌瘤：包括 4 0例富于细胞型子宫平滑肌瘤、13例奇异型子宫平滑肌瘤、4例核分裂活跃型子宫平滑肌瘤、10例不典型子宫平滑肌瘤及 3例恶性潜能未定型子宫平滑肌瘤的p2 1WAF1/CIP1和P5 3蛋白的表达。

Results: The TRAP positive multinuclear cells were induced from rat marrow mononuclear cells after inoculating for 5 clays. RT-PCR analysis showed that ASIC1, ASIC2 and ASIC3 mRNA were present in the osteoclasts, and the corresponding proteins expressions were detected.

结果：诱导5d后可见抗酒石酸盐酸性磷酸酶阳性多核细胞出现，大鼠破骨细胞在mRNA和蛋白水平均可检测到ASIC1、ASIC2和ASIC3基因的表达产物。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐