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In conclusion ,the results of this study showed that the inducing agent HMBA could effectively inhibit the proliferation activity of the human ostosarcoma MG-63 cells,cause the arrest of G0/G1 phase, reverse the malignant phenotype characters,upregulate expression of the osteoblat-like phenotype markers including collagen,osteocalcin,osteonectin , facilitated the formation of bone nodule .

本研究的结果表明HMBA能够有效抑制人成骨肉瘤MG-63细胞增殖活动,改变肿瘤增殖核抗原表达,将细胞周期阻滞在G0/G1期,逆转人成骨肉瘤细胞恶性表型特征,在促进终末分化标志物Ⅰ型胶原纤维蛋白、骨钙蛋白、骨粘素的表达同时,促进骨结节生成,从而说明HMBA能够诱导人成骨肉瘤MG-63细胞终末分化,并且Rg1、BPO-L、Rg1+BPO-L以及Rg1+HMBA等均具有终末诱导分化能力。

Results After induction by VEGF or PDGF for 1 week,most of adherent mononuclear cells became round and small, while few grew into spindle-shaped cells.

结果新鲜脐血分离单个核细胞培养1周后贴壁细胞的EPCs特异的Dil标记乙酰化低密度脂蛋白鉴定为80%阳性。

EMSA results show that the binding patterns between the core DNA sequences of DNase Ⅰ supersensitive sites (HS2, HS3 and HS4) in LCR and nuclear matrix proteins isolated from Hu-induced and uninduced-HEL cells are quite different.

运用凝胶电泳阻抑法揭示了用羟基脲诱导与非诱导的HEL细胞内,与LCR中的DNaseⅠ超敏感点(HS2,HS3和HS4)核心DNA序列结合的核基质蛋白发生了显著的变化。

Methods VEGF in the serum of 60 patients with RA and 28 healthy control subjects was quantified by our highly sensitive enzyme-linked immunoad sorbent assay, and its relationship to clinical and laboratory variables was analyzed . Synovial specimens were obtained from 1 RA and 1 OA patients undergoing synovectomy.

体内实验:用酶联免疫吸附方法检测了60例RA和28例健康对照血清中的VEGF值,并检测其他活动性指标及血清中特异性自身抗体:抗核周因子抗体、抗角蛋白抗体、抗环瓜氨酸肽抗体,分析它们之间的关系。

Yang E,Korsmeyer SJ.Molecular thanatopsis:a discourse on the BCL-2 family and cell death.Blood,1996,88:386[3] Campos L,Rouanlt JP,Salido O,et al.High expression of BCL-2 protein in acute myeloid leukemia cells is associated with poor response to chemotherapy.

BCL-2是新一类癌基因——凋亡调控基因BCL-2家族的代表,其编码26kd的蛋白存在于线粒体、核膜及内质网,与同家族的其它成员组成不同比例的同、异二聚体而影响凋亡启动[6]。

Expression level of NR4A1 in theca cell of mature follicle was significantly higher than in that of growing follicle P

核受体NR4A1蛋白的表达与卵泡的发育成熟及黄体的萎缩有明显的相关性。

These kinases indirectly modulate CREB target gene transcription and expression by affecting TORC phosphorylation and their subsequent distribution between nucleus and cytoplasm.

这些蛋白激酶通过影响TORC的磷酸化水平,改变其在核-质中的分布,间接影响CREB目的基因的转录与表达。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下:一、tau蛋白过度表达和聚积对细胞形态的影响:倒置显微镜下观察两种细胞的形态,发现N2a/wt细胞的突起多而长,而N2a/tau40细胞胞体变圆,突起明显缩短;免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍,免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光,tau主要分布在核周和突起起始部分的胞质内,而N2a/wt细胞内的荧光很弱。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7(MDA-7)的克隆及功能研究:利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列,经测序鉴定序列与文献报道一致后,与真核表达载体pcDNA3.0连接,构建pcDNA3-MDA-7表达载体,瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA,RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达;转染48h后测定细胞增殖和活力,MDA-7可选择性抑制肿瘤细胞的增殖,对A549、Hela和HepG2细胞的抑制率分别为25%、20%和19%,但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后,以G418筛选2周后计数单层细胞集落形成数,计数仅为转染pcDNA3.0空载体的细胞的30%左右。

Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells.

Rab蛋白是一类小分子的GTP酶,调节真核细胞内的小泡运输。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

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