蛋白核

The cells were transfected with the eukaryotic expression vector αS1-LA-psv and the activity of β-galactosidase was detected after transfected from 24 h to 120 h. Human alpha lactalbumin was detected at 72 h after transfected too.

将构建的真核表达载体αS1-LA-psv 转染奶牛乳腺上皮细胞，培养24～120 h均检测到了β-半乳糖苷酶的表达；培养72 h检测到细胞中人α-乳白蛋白的表达，表达量约为

AIM: To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani anddetectexpressionofthegeneinNIH3T3cells.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

METHODS: Amastin gene was amplified from nuclear DNA of Leishmania Donovani isolates and cloned into an eukaryotic expression vector pcDNA31.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

Objective To investigate the role of antiapoptotic Bcl-x protein in megakaryocyte differentiation and maturation.

目的 研究抗凋亡蛋白Bcl-x在巨核细胞分化和成熟过程中的作用。

In a cocultivation experiment, hepatoma derived HepG2 cells, but not NB4 cells, methylate radiolabeled iAs〓.

与iAs〓相反，MAs〓O既不降解PML-RARα蛋白或恢复PML核体正常的分布模式，也不诱导NB4细胞分化。

Direct immunofluorescence by con-focus micrography also indicated that il-10 increase the expression of membrane-bound blys protein.

直接免疫荧光法检测外周血单个核细胞膜结合型blys蛋白显示，il-10刺激后blys表达增强。

Objective To investigate the change of GTP binding protein TC10 in the facial motoneuron regeneration.

目的 探讨大鼠面神经切断后不同时期小GTP结合蛋白TC10mRNA在面神经核中不同时期的表达变化。

Part Ⅱ Objective: To study the immunoprotective and the immunotherapic effects of the new nucleic acid vaccine of the hepatitis B: pVAX1-C3d-S2S; respectively in normal and HBV transgenic mice after being inoculated intra- muscularly with this Eukaryotic Expression Plasmid, such as the in situ production of the expressed protein in muscle tissue section and specific humoral immune response in normal mice、the variation of specific antigen、antibody and HBV DNA, especialy the changes of specific antigen in liver.

第二部分新型乙肝核酸疫苗pVAX1 C3d-S〓S免疫预防效应和治疗作用的研究目的：研究含有新型分子佐剂补体C3d的HBV adw血清型的乙肝核酸疫苗重组真核表达质粒pVAX1-C3d-S〓S接种正常BALB／c小鼠和HBV转基因小鼠后，其免疫预防作用（接种局部肌肉中特异性抗原蛋白的表达和所诱生的体液免疫应答规律）以及免疫治疗作用（血清中特异性抗原的表达、特异性抗体的产生、HBV DNA的消长和肝组织中抗原表达的变化）。

Objective To investigate Bcl-2 expression in the peripheral blood mononuclear cells and its clinical significance of myasthenia gravis .

目的 探讨重症肌无力患者外周血单个核细胞Bcl-2蛋白表达及其临床意义。

Objective: To test GPⅡb/Ⅲa and GPⅠb/Ⅸ specific autoantibodies of thrombocytopenic patients by MAIPA assay, evaluate the usefulness of MAIPA for the differential diagnosis of immune and nonimmune thrombocytopenia.

在血小板和巨核细胞膜表面存在大量的糖蛋白分子，它们不仅是血小板发挥功能所必需的，而且在免疫性血小板减少的发病中占重要地位。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。