蛋白核

Eukaryotic genomes consist of a number of chromosomes whose DNA is associated with different kinds of proteins.

真核基因组包含许多染色体，而它的脱氧核糖核酸由不同种类蛋白组成的。

Objective: To observe the effect of expelling wind and expectorant Chinese medicine on the expression of LOX-1 mRNA and NF-κ B mRNA induced by angiotensin Ⅱ in human umbilical vein endothelial cells.

目的 观察搜风祛痰中药对AngⅡ诱导体外培养人脐静脉内皮细胞凝素样氧化低密度脂蛋白受体1(LOX-1)与核因子－κB基因表达的影响。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

Ambs S,Dennis S,Fairman J,Stillman IE,Lombardo M.Inhi-bition of tumor growth correlates with the expression level of a human angiostatin transgene in transfected B16F10melanoma cells ［J］.

本实验将大鼠血清白蛋白分泌信号正确连接在endostatin基因的碳末端，成功构建了真核表达载体pcDNA3-SE，并利用Lipofectamine介导转染C6胶质瘤细胞。

Interactions of Alzheimer amyloid-beta peptides with glycosaminoglycans effects on fibril nucleation and growth.

相互作用的阿尔茨海默-淀粉样蛋白与纤维成核和生长糖胺的影响。

Methods The BHD vector and the small interfer RNA targeting BHD was constructed and transfected into the cell line A549. The expression of BHD was examined by immunofluorescence. Transwell test was used to detect the invasion ability of A549 cells. Results After transfected BHD vector into A549 cells, immunofluorescence showed the expression of Folliculin located in cellular plasma.

制备BHD真核表达质粒，转染肺腺癌细胞，用免疫荧光法检测Folliculin蛋白在肺癌细胞中的表达位置；筛选sRNAi片段，选取有效抑制片段转入肺癌细胞，与转入BHD质粒的肺癌细胞对比，用Transwell实验了解BHD基因对肺癌细胞运动方面的影响。

Result: 1.(1) Three strips come to appear in the position of 6.0kb, 5.4kb and 600bp after gelose electrophoresis and their size accord with recombined plasmid DNA, pcDNA3 plasmid and VEGF165 gene accordingly.(2) Pure degree of the pcDNA3-VEGF165 recombined plasmid is: OD260/OD280=1.87. Its

在进行了重组质粒的扩增、提取、纯化、鉴定、兔MSCs的原代及传代培养以及RT-PCR、Western Blot 转染检测后，实验取得了令人满意的结果，证实了MSCs 能在体外有效转录外源VEGF 基因并分泌出VEGF 蛋白，同时也说明pcDNA3-VEGF165重组真核表达质粒是有效的。

The human globin gene family is a good model for eukaryotic gene expressionregulation research.

人类珠蛋白基因家族是真核基因表达调控研究的良好模型。

Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.

目的 构建狂犬病病毒糖蛋白基因的真核细胞表达载体，并在COS-7细胞中表达。

This review deals with the active site structure, reactivities and chemical mimics of the binuclear copper enzymes, particularly hemocyanin and tyrosinase .

本文综述了双核铜酶，特别是酪氨酸酶、血蓝蛋白的结构，键合氧、活化氧的性能及有关仿酶模型的研究进展。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。