蛋白核

In eukaryotic cells, DNA is located in nucleolus in the form of chromatin by combining with histone proteins.

在真核生物中，DNA分子缠绕在组蛋白上形成染色质保存在细胞的细胞核中。

Western-blot and immunohistochemical assay methods were employed to detect the expression of FHIT. Apoptosis and cell cycle of cell were analyzed by FCMS. Morphological changes were observed by compound and electron microscope.

然后用流式细胞仪，普通光镜及透射电镜对获得稳定FHIT蛋白表达的转染组细胞和空真核表达质粒转染组细胞及未转染的亲本细胞凋亡的状况进行了考察，另外还通过流式细胞仪检测了三组浙江大学硕士学位论文细胞生长周期的惰况。

The recombinant eukaryotic expression vector pcDNA3.1-Myc-His B/tPA can stable express tPAcDNA in transfected hUVEC cell strains and can secret compt tPA protein. Experiment III The Effects of Transfected tPA Gene on Vascular Smooth Muscle Cells Proliferation and Migration in VitroObjective To investigate the effects of transfected tPA gene on vascular smooth muscle cell proliferation and migration in vitro and to provide a basis for further study on preventing vein graft restenosis.

结论构建的pcDNA3.1-Myc-HisB/tPA基因新型真核表达载体，能在HUVEC中表达，其表达具有高拷贝、稳定性好的特点，获得的表达蛋白活性较高，提示本载体和稳定表达tPA基因的人脐静脉内皮株能够作为基因治疗工具用于哺乳动物的实验研究。

It is conserved in various eukaryotes, suggesting an important role of the protein.

它的同源蛋白存在于多种真核生物中，暗示了功能的重要性。

The bioinformatics analysis showed that the gene had the highest homology with the gene of Penicillium janthinellum, which reached up to 76%. The first 26 amino acids might be a signal peptide sequence with the hydrophobicity of up to 2.63. The CBHⅠ gene consisted of the catalytic domain, convergence zone and cellulose-binding domain and its tertiary structure was mainly composed of 3-sheets.

分别扩增到鹅源草酸青霉F67纤维素酶CBHⅠ基因片段A(EU574736)、5'端序列B1(EU603295)、3'端序列B2(EU652768)及全长基因C(EU727171)，并成功构建真核表达载体pPIC9K-CBHⅠ；生物信息学分析表明，该基因蛋白序列与微紫青霉氨基酸序列同源性最高，达76%，前26个氨基酸为信号肽序列，疏水性可达2.63，由催化功能域、衔接区和真菌性纤维素结合域构成，其三级结构主要为β-sheet。

Actin is a highly conserved protein, which exists in almost all eukaryotic cells and has been proved to be essential for many cellular functions such as movement, morphology, growth, cytokinesis as well as other crucial events.

肌动蛋白是一类高度保守的蛋白质，存在于所有的真核细胞中，参与细胞分裂、运动、迁移、形态的维持、生长等许多重要生理活动。

Methods The DIO and DIO R rat models was established by high fat diet. The rats were killed in 2 and 12 weeks. Situ hybridization and immunohistochemistry were used to measure the expression of NPY mRNA and protein in the rats.

高脂饮食建立DIO与DIO-R大鼠模型，分别于2周、12周末处死，采用原位杂交和免疫组化方法检测下丘脑弓状核神经肽Y的 mRNA和蛋白表达情况。

Methods The DIO and DIO-R rat models was established by high fat diet. The rats were killed in 2 and 12 weeks. Situ hybridization and immunohistochemistry were used to measure the expression of NPY mRNA and protein in the rats.

高脂饮食建立DIO与DIO-R大鼠模型，分别于2周、12周末处死，采用原位杂交和免疫组化为法检测下丘脑弓状核神经肽Y的mRNA和蛋白表达情况。

These studies suggest that different eukaryotic pathogens use equivalent targeting signals to deliver their effector proteins.

这些研究说明，不同真核病原体可利用等价的定位信号来提供它们的效应子蛋白。

Results There were significant differences between MDS and CAA in Hb, red cell distribution width-coefficient variation, immature reticulocyte fraction, BPC, the ratio of G1 (the sum percentage of myeloblast and premyelocyte) to G2 (the sum percentage of neutrophilic myelocyte and metamy-elocyte), the ratio of E1 (the sum percentage of proerythroblast and early erythroblast) to E2 (the sum percentage of intermediate erythroblast and late erythroblast), megakaryocyte count,erythroblast PAS, neutrophil alkaline phosphatase, and serous levels of indirect bilirubin,lactose dehydrogenase, folic acid, VitBl2 and ferritin.

结果 MDS患者血红蛋白，红细胞体积分布宽度。变异系数、未成熟网织细胞比率、血小板计数、骨髓原始细胞及早幼粒细胞之和与中性中幼粒细胞及中性晚幼粒细胞之和的比值、原始红细胞及早幼红细胞之和与中幼红细胞及晚幼红细胞之和的比值、巨核细胞计数、有核红细胞糖原染色阳性率和阳性指数、中性粒细胞碱性磷酸酶染色阳性率和阳性指数、血清间接胆红素、乳酸脱氢酶、尿酸、叶酸、维生素B12(VitB12)、铁蛋白水平等常规实验室指标与CAA患者比较差异有统计学意义。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。