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When Shigella flexneri 2a 2457T is cultured at 30℃, it's phenotypically avirulent; when grown at 37℃, its virulence improves greately. There is clear evidence that the temperature-dependent expression of the virulence genes is controlled by H-NS protein.

痢疾杆菌在30℃时不具有侵袭力,37℃时毒力明显增强,已有的研究表明这种依赖于温度的毒力基因的表达受H-NS蛋白的调控。H-NS是一种重要的类核相关蛋白,在革兰氏阴性菌中普遍存在,能够调控大量基因的表达。

The expression of SRp38 in mouse retina was investigated by Western blot and immunohistochemistry analyses. The result shows that the expression of SRp38 proteins in mouse retina is region-specific, with extensive distribution in the outer and inner plexiform layers, inner nuclear layer and ganglion cell layer, but no expression in outer nuclear layer.

利用Western blot 以及免疫组织化学方法研究了SRp38蛋白在小鼠视网膜中的表达以及分布情况,结果显示,SRp38蛋白在视网膜中的表达具有区域特异性,在外网层、内核层、内网层以及节细胞层中均有表达,而在外核层无表达。

OBJECTIVE: To construct a novel adenoviral eukaryotic expression vector that can co-express mutant hypoxia-inducible factor-1 alpha (HIF-1α) target protein and humanized Renilla reniformis green fluorescent protein reporter molecule under normoxic conditions.

目的:构建能够同时表达突变型低氧诱导因子1α(hypoxia inducible factor 1 alpha,HIF-1α)目的蛋白和人源化绿色荧光蛋白(human renilla reniformis green fluorescent protein,hrGFP)报告分子的新型腺病毒真核细胞表达载体。

Methods:The conservative region of mTOR gene was inserted into pLXIN reversedly, then the vector was packaged in PT67 cells by transfection with lipofectamine and transfected to VSMC. The efficiency of antisense inhibition was verified,and the changes of p70S6k and 4E-BP1 were also determined at the same time. The proliferation activity was determined by flowcytometry and MTT.

采用脂质体介导转染包装细胞后构建mTOR的反义重组逆转录病毒载体(pLXIN-AmTOR)感染VSMC,检测mTOR及其下游底物包括真核细胞启动子4E结合蛋白1(4E-BP1)和核糖体蛋白S6激酶(p70S6k)的表达变化,以及VSMC细胞增殖周期和增殖活性的改变。

The precise sequence and the substructural context of an NLS or NES defines its specificity for the various Kaps (21 )Furthermore, the receptorproteinand receptor-RNA interactions that mediate

一个核定位信号序列和核输出信号序列的精确序列和结构成分,决定了它对各种各样Kaps蛋白的专一性,而且,调节物质定位的蛋白受体和RNA受体相互作用由各种转录和翻译后加工所控制,这考虑到了介质本身精确运输的调节。

The interaction was not dependent on the tetrameric structure and enzyme activity of GNMT. Further, we identified GNMT can promote nuclear accumulation of ARRB1 in HeLa.

免疫荧光共定位实验显示,当ARRB1与GNMT共转染HeLa细胞后,ARRB1蛋白的亚细胞定位发生改变,表现出与GNMT蛋白共定位,有入核的趋势。

Results TNFα-induced ICAM-1 expression in HK-2 cells was increased in both protein and mNRA levels( P .01). NFkB inhibitor N-tosyl phenylalanine chlormethyl ketonecould inhibit these effects of TNFα.

方法用 HK-2 细胞作靶细胞,用细胞酶联免疫吸附法和 Northem 杂交观察 ICAM-1 的蛋白和基因的表达,以电泳迁移率变动法测定转录因子核因子 kB和激活蛋白 1(AP-1)的活性。

Researchers at the University of California, Los Angeles, and The Hospital for Sick Children in Toronto monitored neurons in the lateral amygdalae (two almond-shaped regions on either side of the midbrain associated with learning and memory) of mice to see whether the presence of the CREB protein plays a key role in signaling brain cells to make memories.

洛杉矶加利福尼亚大学及多伦多儿童医院的研究者们对小鼠外侧杏仁核部位(该部位是主管学习和记忆的中脑两侧杏仁状的区域)进行监测,以研究CREB蛋白(cAMP反应元件结合蛋白)是否在脑细胞形成记忆过程中起着至关重要的作用。

objective to investigate the mechanism of the expression of c-fos protein by observing the changing tendency of c-fos protein in basolateral nucleus of rat amygdale after intermittent swimming exercise.

摘 要:目的:观察间歇性负重游泳训练后大鼠杏仁基底外侧核内c-fos蛋白表达的变化,探讨间歇性游泳训练对c-fos蛋白表达的诱导机制。

Calmodulin A Ca2+binding protein found in high levels in eukaryote cells.

钙调蛋白:在真核细胞中高水平存在的一种钙离子结合蛋白。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。

The shamrock is the symbol of Ireland and of St.

三叶草是爱尔兰和圣特里克节的标志同时它的寓意是带来幸运。3片心形叶子围绕着一根断茎,深绿色。