蛋白核

Coll. Methods Insert the synthetic SARS-CoV N protein gene into vector pUC-18,ligate to plasmid pET28 and transform to E.

方法将已合成的SARS-CoV N蛋白基因片段，克隆至pUG18载体，与pET28质粒连接，转化大肠杆菌，进行SARS-CoV核衣壳融合蛋白表达。

Methods: Thirty adult male Wistar rats were randomly divided into three groups: the operation group, the transplantation of MSCs group and the injection of saline group. Functional outcome measurements were performed based on the modified neurological severity score at day 1, 7, 14, 21 and 28 after MACO. The survival, migration, expression of Nestin, glial fibriliary acidic protein and neuronspecific enolase of 5bromo2deoxyuridinelabeled MSCs were detected by immunohistochemical double staining.

线栓法建立左侧大鼠MCAO模型，随机分为3组：手术组、MSCs 移植组、生理盐水组。5溴2脱氧尿核苷标记的MSCs移植后，采用改良神经功能损伤评分系统评价大鼠神经功能恢复情况；应用免疫组织化学双染技术检测MSCs的存活、迁移及其巢蛋白、胶质纤维酸性蛋白和神经元特异性烯醇化酶的表达。

In order to understand the function of the Rab protein in ciliates, the coding region of Eorab1f was obtained from macronuclear DNA of Euplotes octocarinatus. Sequence analysis revealed that the gene contains 624 bp and two in-frame TGA codons in the coding region.

为进一步研究该家族成员的功能，本研究从八肋游仆虫大核基因组中克隆得到Rab蛋白家族中一个新Rab蛋白基因(命名为Eorab1f)的编码区。

The isonucleus-allocytoplasmic materials ms-Xinong 1376,-Xinong 1376, and ms-Xinong 1376 were used to investigate the protein expression profile in mononucleated anaphase anthers of wheat (Triticum aestivum L.) after silver staining and analysis by PDQuest software. About 320 to 350 distinct protein spots were detected reproducibly.

为了研究小麦雄性不育蛋白表达机制，以具有异质同核的3个亲本：即遗传型不育系ms-西农1376、对应的保持系-西农1376和生理型(化学杀雄剂SQ-1诱导)雄性不育系ms-西农1376为材料，利用IEF/SDS-PAGE凝胶电泳技术，对发育到单核后期的花药全蛋白特异性进行了比较分析，得到320~350个清晰的蛋白点。

After 48h exposure to 50mg/L ox-LDL, under the fluorescent microscope partial cells Showed typical apoptotic morphological changes such as nacarat fluorescent body shrinking and fragment of the nucleus. At the same time, the cellular cholesterol content also increased obviously, and many lipid droplets accumulated in cells.

RAW264.7细胞经50mg／L ox-LDL处理48h后，AO／EB染色显示明显的细胞凋亡形态学特征，出现核固缩、碎裂，同时细胞内胆固醇含量显著增加(由76.3μg／mg蛋白上升至368.5μg／mg蛋白)，油红O显示细胞内有大量的脂滴存在。

Microscopically,tumor showed an invasive growth and nodulous arrangement and was made up of two kinds of cells,relativepart...

免疫组化示：上皮膜抗原，细胞角蛋白，波形蛋白均强阳性，S100，CD34弱阳性，CD20、CD3核可疑阳性，其余多项免疫组化结果均呈阴性。

Results There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligodendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group.

结果 免疫组织化学结果显示，Nogo-A免疫阳性染色的光密度值与肿瘤细胞核分裂、核异型比例呈明显的负相关，对蛋白印迹试验结果进行光密度分析也发现少突胶质细胞瘤中的Nogo-A蛋白条带灰度值明显高于间变型少突胶质细胞瘤中的灰度值。

This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineage-independent evolutionary events such as expansion of gene families, orthology relations and protein evolution.

这次全面分析包含了三种动物的基因组序列和蛋白产物以及它们与人类疾病相关性分析，重复序列，哺乳动物同源染色体区域和重排断点的比较基因组研究，祖先核型的重建，产生现有种群的事件，变异率以及种系特异和不依赖种系的事件如基因家族扩展，同源关系以及蛋白进化。人类19号染色体序列被破译了。

This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineage-independent evolutionary events such as expansion of gene families, orthology relations and protein evolution.

这次全面分析包含了三种动物的基因组序列和蛋白产物以及它们与人类疾病相关性分析，重复序列，哺乳动物同源染色体区域和重排断点的比较基因组研究，祖先核型的重建，产生现有种群的事件，变异率以及种系特异和不依赖种系的事件如基因家族扩展，同源关系以及蛋白进化。生物谷评论：人类19号染色体序列被破译了。

These changes were prevented by genistein (a protein tyrosine kinase inhibitor) and antioxidant N-acetyl-L-cysteine, but promoted by sodium orthovanadate (a protein phosphatase inhibitor), which were administered to the SD rats 20 min before ischemia.

进一步的研究表明，缺血前20 min腹腔注射给药，然后缺血30 min，发现蛋白酪氨酸激酶抑制剂染料木黄酮和抗氧化剂N-乙酰半胱氨酸能显著地抑制核内STAT3的磷酸化水平及DNA结合活性的增加(磷酸化水平从2.3和2.5倍分别降为1.2和1.4倍， DNA结合活性则从2.8和3.7倍分别降为1.1和1.5倍)，而蛋白酪氨酸磷酸酶抑制剂矾酸钠则能明显地促进他们的增高(磷酸化水平从2.0倍增到3.4倍， DNA结合活性从3.1倍增为5.1倍)。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。