蛋白核

METHODS: Cerebral cortex cells of 8-week aborted human fetus were harvested and cultured in vitro to obtain human embryonic NSCs. Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4 点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶／微管相关蛋白2和5'-溴脱氧尿嘧啶／胶质纤维酸性蛋白免疫组织化学双染。

The human and mouse Period1 genes: five wellconserved Eboxes additively contribute to the enhancement of mPer1 transcription ［J］.

研究发现成熟的67 kD蛋白是由2个37 kD的前体蛋白聚和而成［10］，可位于细胞表面［11］、胞浆［12］和胞核内［13］。

The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞，用G〓选择压力筛选，细胞免疫荧光检测表明，pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白，这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

The minichromosome maintenance proteins are extensive existenced and conserved throughout archae to eukaryotic cells.

微小染色体维持蛋白（minichromosome maintenance proteins，MCMs）是一类从太古代生物到高等真核生物广泛存在的高度保守的蛋白。

The efficacy of this technique for protein mapping was demonstrated by the mass spectral analyses of the peptide fragmentation of several proteins, including lysozyme, albumin, conalbumin, and ribonuclease A.

我们利用四种蛋白质，溶菌蛋白、白蛋白、伴蛋白和核醣核酸酶A的胜肽片段之质谱分析而证明这个方法是非常有效率的。

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果：在绒毛组织中，IL-8蛋白定位于绒毛上皮细胞的细胞质内，且病例组的表达明显高于对照组；在蜕膜组织中；病例组蜕膜细胞的胞质内可见IL-8蛋白表达，且高于对照组；H-E染色可见病例组绒毛组织的滋养层变薄，细胞变性甚至坏死、嗜酸性增强，绒毛中轴纤维化程度增强；蜕膜组织中蜕膜细胞失去细胞间连接，部分蜕膜细胞解体、核消失。

Effects of deguelin on the regulation of nucleoporins and nucleophosmin in Jurkat leukemia cells in vitroObjective: To study the antileukemia effects of deguelin on leukemia cells, and explore the possible mechanisms to provide more theories for clinical application.

第一部分鱼藤素对Jurkat白血病细胞核孔蛋白与核磷蛋白的调节作用目的：研究鱼藤素对白血病细胞增殖和凋亡的影响，并探讨其可能的作用机理，为其临床应用提供理论基础。

NCBI database searching and predicting by SMART show these genes encode proteins invovling in the regulation of protein kinase activity, vesicular transport, protein degration and endonuclear regulation .

经NCBI数据库检索分析和功能域预测，发现四种基因编码的蛋白与蛋白激酶调控，膜泡运输，蛋白质降解以及核内调控有关。

Gambogic acid presents powerful anti-leukemia effects through both proliferation inhibition and apoptosis induction. HL-60 cell line was the most sensitive one to gambogic acid of all three leukemia cell line. However, the exact mechanism may vary from one cell line to another.

第三部分藤黄酸对Jurkat白血病细胞核孔蛋白与核磷蛋白的调节作用目的：研究藤黄酸对白血病细胞增殖和凋亡的影响，并探讨其可能的作用机理，为其临床应用提供理论基础。

With confocal laser scanning microscopy, it was found that the mutant type p53 protein was located in the cytoplasm while the wild type p53 protein was located in the karyon.

用激光共聚焦显微镜观察到突变型p53蛋白定位于胞浆，而野生型p53蛋白则定位于胞核内。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐