蛋白核

The production of human alpha lactalbumin was 0.64 g/L approximately. The results indicate that the bovine mammary epithelial cell line have the ability of expressing exogenous gene, the 5′ flanking sequence of bovine αS1 casein gene has the effect of regulating gene expression specifically and the vector αS1-LA-psv which was constructed can coexpress alpha lactalbumin and β-galactosidase.

实验结果表明，建立的奶牛乳腺上皮细胞系具有外源基因表达活性，得到的牛αS1-酪蛋白5′调控序列能作为启动子指导外源基因的表达，构建的真核表达载体能在体外培养的牛乳腺上皮细胞中同时表达人α-乳白蛋白和β-半乳糖苷酶。

Objective Consequently, by constructing the pcDNA3-HA-hdacl, a HDAC1 eukaryotic expression vector, we were to study the interaction between HDAC1 and ERa and ERB in vitro and the effects of HDAC1 on the ERa and ERB at the protein level. So we can partly reveal the multiplication, differentiation and cancerization of lacteal gland cells.

目的 本文意在通过构建可在真核细胞中表达的重组质粒pcDNA3-HA-hdacl，研究组蛋白去乙酰化酶1(HDAC1)和雌激素受体ERα和ERβ在体外的相互结合作用，以及HDAC1对ERα和ERβ蛋白水平的影响规律，从而在一定角度揭示乳腺细胞的增殖、分化和癌变的过程，为更好地寻找治疗癌症的药物靶标打下基础。

Methods The activity of PTK, PKCα,βI ,βII ,γ,ε,ξ and the active station of NF-κB in large bowel carcinoma tissues was detected by using membraneous conjugated method, laser scanning confocal microscopy and immuriohistochemistry respectively in the animal model of nude mouse transplantation tumors of large bowel carcinoma.

以大肠癌裸鼠移植瘤为动物模型，用膜结合法、激光共聚焦显微镜和免疫组织化学分别测定了大肠癌组织蛋白酪氨酸激酶、蛋白激酶Cα、βI、βⅡ、γ、ε和ξ的活性以及核因子-κB的活化状况。

OBJECTIVE: To explore the feasibility to construct human bone morphogenetic protein-2 eukaryotic expression vector labeled with green fluorescent protein.

目的：验证构建绿色荧光蛋白标记的人骨诱导形成蛋白2真核表达载体的可行性。

Jejunal casein perfusion enhanced NTS c-Fos expression, but did not increase the volume of pancreatic juice and protein.

空肠内酪蛋白灌注可促进延髓孤束核c-Fos表达，但不增加胰液分泌量和胰液蛋白含量。

Primary sequence analysis and in vitro transcription/translation of ECPsp have revealed that it is a potential substrate for human signal peptide peptidase, an intramembrane protease located in endoplasmic reticulum.

此外，以小型干扰核醣核酸的方式压制人类蛋白质前导序列水解脢的表现后，表现嗜伊红血球阳离子蛋白前导序列融合蛋白的人类上皮肿瘤细胞也出现生长抑制的情形。

Eukaryotic nuclear proteins and viral proteins contain NLS, and the protamine is widely used as a natural NLS peptide.

NLS存在于真核细胞核蛋白及病毒蛋白中，鱼精蛋白是应用较为广泛的天然NLS类短肽。

And target protein can be secretively expressed in Pichia pastoris, this is helpful for separation and purification of target protein.Therefore this research intends to compare expression of Core protein in E. coli with in Pichia pastoris.

与大肠杆菌相比，毕赤酵母是单细胞真核生物，既具有生长快的特点，又能有效克服大肠杆菌系统缺乏蛋白翻译后加工、修饰的不足，表达外源蛋白可分泌到胞外，便于产品的分离纯化。

In addition, the silica-precipitating and templating roles of protamine were elucidated, and the mediation mechanism of organic molecules in biomimetic silicification was also tentatively analyzed.

最终确定以羧甲基纤维素钠为仿生微囊的液核，制备出具有海藻酸/精蛋白壳膜和精蛋白/氧化硅杂化壳壁的微囊，并用于包埋GUS，催化黄芩苷转化为黄芩素。

Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats. After 1 and 2 weeks, rats were sacrificed. Adjacent sections were doubly stained by BrdU/microtubule-associated protein 2 (MAP-2) and BrdU/glial fibrillary acidic protein.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐