蛋白核

Compared to wild-type plants, the onset of flowering was advanced. The results of this study may be useful in the mulberry gene engineering and the results may be helpful to study the regulation of SBPase.Rubisco activase is a soluble chloroplast protein, coded in nucleus, and has the activation of Rubisco in photosynthetic autotrophs.

本研究为桑树基因工程提供了有效的候选基因，为深入研究SBPase的分子调控机制奠定了基础。1，5-二磷酸核酮糖羧化酶活化酶广泛存在于光合生物中，它是一种由核基因编码的叶绿体蛋白，具有调节Rubisco活性的功能。

Some exudation and bleedings happen in the pulmonary alveoli, some inflammatory cells infiltrate into lung tissues; in the serum AMY, ALP, TNF-α, IL-6 increases obviously high, COX-2 expression increase; The expression of NF-κB in big rat lung of SAP increase, and the nucleus is thick dyed, it explain that the NF-κ moved into the nucleus; Stimulate the inflamatory reaction.

我们的实验中见到SAP组的大鼠肺脏组织细胞腔扩大、肺泡壁断裂，有的出现肺大泡，肺泡内伴有渗出及少量非出血，肺组织可见咽细胞浸润；血清中AMY、ALP、TNF-α、IL-6明显增高；在SAP组大鼠肝细胞、肾小管上皮细胞、肺脏小气管及肺泡上皮上COX-2表达增加；NF-κB在SAP大鼠肺脏中表达增加，并且出现核浓染，说明NF-κB前移入核；启动炎症反应等一系列反应SAP大鼠受损伤的肺组织中AQP-1无论在mRNA水平上、蛋白水平上还是免疫组化染色病理学检测上都明显下降。

The biological characteristic of many vertebrate parvoviruses have been clarified, for example the genome organization, the structure of capsids protein of virus particle, and the relationship between the structure and its function.

它与其它细小病毒一样，病毒粒子无包膜，呈球状二十面体对称，直径22nm，基因组为单链线状DNA分子，含有五种结构蛋白，嶂螂浓核病毒基因组全长为5454个核昔酸，于C含量为38.13%。

When chromatin is reproduced, a stretch of DNA already associated with nucleosomes is replicated, giving rise to two daughter duplexes.

体外合成核小体要从考虑自由DNA和组蛋白的组装过程开始，但体内的核小体仅在DNA复制时形成。

Nuclear factor kappa B is a kind of eukaryon cell transcription factor which is transported to the cell nucleus by extracellular stimulus such as LPS, shock and so on through signal transmission pathway. It promotes and regulates the gene transcription of some inflammatory mediators and it closely relates to ALI.

核因子-кB是一种基因转录调节蛋白，内毒素、休克等细胞外刺激通过信号转导通路，引起NF-кB核易位，启动和调节多种炎症介质的基因转录，其活化与ALI的发病密切相关。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间，进行髓核细胞活力测定；爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察；MTT 法绘制髓核细胞生长曲线，并行原代及第2 代细胞透射电镜观察，对体外细胞的生物学特性进行研究。

The expression of c-fos protein in the nucleus of tractus solitarius and dorsal motor nucleus of vagus of the central bulb was observed by immunohistochemical method.

分别于刺激后0.5、l、2和5h处死大鼠，以免疫组化方法观察c-fos蛋白在延髓孤束核和迷走神经运动背核中的表达。

Aim:(1) The purpose of this study is to examine whether protein tyrosine kinase and AMPA receptor in the nucleus tractus solitarius and nucleus ambiguus were involved in the regulation of normoxia and hypoxia respiratory response by microinjection of PTK inhibitor and non-NMDA receptor antagonist;(2) To observe the change of number of phosphotyrosine immunopositive neurons in NTS and NA before and after hypoxia using immunohistochemical methods and whether tyrosine phosphorylation and GluR1 in postsynaptic membrane co-localize in NTS and NA neurons.

目的：(1)观察向家兔脑干孤束核或疑核内微量注射酪氨酸蛋白激酶阻断剂和谷氨酸AMPA受体阻断剂对常氧呼吸和低氧呼吸反应的影响，探讨PTK和AMPA受体在低氧呼吸反应中的作用。(2)观察低氧通气后NTS和NA磷酸化酪氨酸免疫阳性神经元的数量变化；观察磷酸化酪氨酸和AMPA受体亚单位GluR1在突触后膜共存的情况，推测在低氧呼吸反应中AMPA受体和PTK信号转导途径的关系。

Histones play a major architectural role, holding the coiled DNA in supercoils or beads, about 10nm in diameter and consisting of about 170base pairs.

组蛋白与 DNA 非特异性的结合，包装成为核小体，核小体彼此连接形成直径约10nm 的串珠结构，包含170个碱基对。

Methods Mammalian expression vectors containing human MGMT gene were tranferred into PBMC in vitro via liposome. Realtime PCR and Western blot were used to detect the expression of MGMT at mRNA and protein levels.

利用脂质体法将含入MGMT基因的真核表达载体导入体外培养的外周血单个核细胞，实时PCR及Western blot检测目的基因mRNA及蛋白水平的表达。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐