蛋白核

Results The reticulin fibre proliferated in all specimen and most of them occured mega karyocytosis.The myelofibrosis was severer along with the progress of CML,while megakaryocyte counts didn′t change significantly.

结果 发现所有患者的骨髓组织均存在网硬蛋白纤维增多，且随CML进展其程度加重；大多数患者巨核细胞过度增生，在CML不同阶段中巨核细胞数比较未见显著差异。

MUC2 apomucin and its mRNA were mainly located in the peri-and supranuclear area of goblet cells of duodenum and column,but no signal in the columnar-type cells.

结果发现，胃粘膜中无MUC2和MUC3基因产物的表达，MUC2核粘蛋白及mRNA主要存在十二指肠和结肠杯状细胞周及核上，柱状细胞中无表达。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

采用荧光定量PCR技术检测EB1基因在人工流产绒毛核型分析正常的胚胎细胞和自然流产绒毛核型分析异常的胚胎细胞中的表达差异；免疫组织化学方法检测EB1蛋白在两者中的表达情况，并用Western blot加以验证。

In eukaryotes, DNA left-handed chirality by the way, wrapped around histone octamer around, the formation of a stable nucleosome structure.

在真核生物中，DNA按左手手征性的方式，缠绕在组蛋白八聚体的周围，形成稳定的核小体结构。

In eukaryotic cells, the fundamental unit of chromatin is nucleosome, which is composed of a histone octamer with two copies of H2A, H2B, H3 and H4 and 146 base pairs of DNA.

真核细胞中，核小体是染色质的基本单位，它由两个拷贝H2A、H2B、H3和H4所组成的核心组蛋白八聚体，及缠绕于其上的146bp DNA构成。

In this study, the DNA fragments coding for amino acids 133～158 of VP1and 20～34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133～158-20～34-133～158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133～158位氨基酸残基确是一重要B细胞中和抗原表位的基础上，依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列，采用真核偏爱密码子化学合成了VP1中编码133～158位氨基酸及VP4中编码20～34位氨基酸这两个抗原表位基因，将其组成133～158-20～34-133～158串联结构，在其5′端加上鼠Igκ链信号肽序列，在翻译调节区加上增强表达的Kozak序列，同时在133～158-20～34-133～158串联结构3'端终止密码子下游加上CpG免疫刺激序列，将这些片段连接后，克隆到真核表达载体pHook-2上，构建成功了DNA疫苗重组表达载体pAS1-E。

Results:(1) The rats produced amphetamine CPP after treated with 2 mgkg^(-1) amphetamine for 4 days. Ketamine and low, middle, high dose rhynchophylline all could eliminate the CPP effect, and the effect of rhychophylline was increased in a dose-dependent manner. Rhychophylline itself could not induce CPP in normal rats.(2) When compared with normal rats, the NR2B expression in nucleus accubens and amygdaloid of the model rats was obviously increased. Ketamine and middle, high dose rhynchophylline could decrease NR2B expression induced by amphetamine, while low dose rhynchophylline did not affect NR2B expression. Rhynchophylline had no effect on RN2B expression in normal rats.

结果：(1)建立了苯丙胺（2mgkg^(-1)，连续4d）诱导的位置偏爱模型，氯胺酮及钩藤碱低、中、高剂量均可消除苯丙胺诱导的位置偏爱效应，随钩藤碱剂量增加其效应加强，且本身无精神依赖性；(2)苯丙胺模型组大鼠伏核和杏仁核NR2B蛋白表达增加，氯胺酮及钩藤碱中、高剂量抑制NR2B表达，低剂量及本身对NR2B表达无影响。

Double immunofluorescence staining revealed that the interaction of BRCA1 protein between cell plasm and nucleus stimulated BRCA1 nuclear export was obvious in different pancreatic tissues. BRCA1 nuclearcytoplasmic shuttling manner was correlated with cancer development.

免疫荧光双重染色法证实BRCA1蛋白在胰腺癌细胞胞质和胞核间互动，这是国内首次发现和报道BRCA1在胰腺癌组织细胞中胞质和胞核间互动现象和其亚细胞分布情况。

Objective To screen possible disease-causing gene CRYAA with 15 cases of congenital cataract.

目的 分析遗传性先天性核性和绕核性白内障与定位於21q22.3的αA-晶体蛋白基因之间的关系。

Histamine deficiency markedly potentiated the acquisition of learning in cued and contextual fear conditioning in the HDCKO mice,but had no effect on cued and contextual fear extinction.However,histamine deficiency potentiated the inhibitory role of morphine withdrawal in cued fear extinction.Consistent with the behavioral results,morphine withdrawal decreased ERK phosphorylation in the amygdala and mPFC but not in the hippocampus of WT and HDCKO mice,and ERK might not be phosphorylad in the amygdale and mPFC of HDCKO mice after the 4th training of cued fear extinction.

但HDCKO小鼠增强了吗啡戒断对线索恐惧记忆消除的抑制作用，与行为学实验结果相一致是，，吗啡戒断能明显降低WT和HDCKO小鼠在线索恐惧记忆消除第4次训练后杏仁核和mPFC脑区的ERK磷酸化水平，但对海马脑区的ERK磷酸化没有作用；HDCKO小鼠在线索恐惧记忆消除训练的第4次训练后杏仁核、mPFC脑区的ERK蛋白没有明显磷酸化激活。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐