蛋白核

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

Keywords: Giardia lamblia; lamin; nuclear division; evolution

中文关键词：贾弟虫；核纤层蛋白；核分裂；进化

"Identification and characterization of autoantibodies against the nuclear envelope lamin B receptor from patients with primary biliary cirrhosis".

&识别和鉴定抗体对核被膜核纤层蛋白B受体例原发性胆汁性肝硬化&。

IPLB LD-652Y-5d cells infected with Lymantria xylina multiple nucleopolyhedrovirus were found that the infection cells were fused together and formed syncytial giant cells. This phenomenon is probably caused by the viral envelope fusion protein in the cell membrane of the infected cells.

以黑角舞蛾核多角体病毒(Lymantria xylina multiple nucleopolyhedrovirus； LyxyMNPV)感染吉普赛舞蛾细胞株(IPLB-LD652Y-5d)后，发生细胞融合形成一大的多核细胞，此细胞融合现象可能与病毒套膜融合蛋白有关。

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术，从猪瘟病毒Shimen株细胞毒中克隆出P80 基因，并进一步把P80 基因连接在真核表达载体pEGFP-C1 上，成功地构建出P80 基因重组真核表达质粒(pEGFP-P80)，为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收，然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化，获得重组质粒，经PCR，Xho I 和Apa I 限制性酶切和序列测定，鉴定为真核表达质粒pEGFP-P80，并且目的基因的插入位置、方向和读码框完全正确，为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

Through a time correlation analysis of spontaneous intensity fluctuations,this technique in conjunction with EGFP as a probe is capable of determining nucleoplasmic viscosity in terms of Stokes-Einstein equation as well as its corresponding analysis of the diffusion coefficient for EGFP in the nucleus.

研究中利用FCS技术测定绿荧光蛋白分子在细胞核内的扩散系数，并且根据Stokes-Einstein关系式计算出核浆粘滞系数，同时实现了对特定生理条件下核浆粘滞系数的跟踪测量。

Here, similarities between archaebacteria and eukarykaryotic otic cells in many aspacts including DNA duplication, transcription, translation and histones, chromatin, proteasome were introduced. Finally, the evolutionary position of archaebacteria and their significance to onery gin of the eukaryotic cells were discussed.

结合国际上对原细菌，尤其是它与真核细胞在许多方面（包括DNA复制、转录和翻译整个中心法则，组蛋白、染色质及蛋白酶复合体等）密切的亲缘关系的研究结果，对原细菌的进化地位及其对揭示真核细胞的起源进化问题的意义进行了探讨。

Oncogenes, existing widely in eukaryotic cells, take part in the processes of cell prolification and differentiation. c-fos and c-jun are two important oncogenes in CNS, and can be rapidly expressed in response to stimulations and injuries of nervous system, Oncogene proteins play very important rules as "nuclear third messengers"during signal transduction in CNS.

癌基因广泛存在于真核细胞内，参与细胞的增殖分化等活动。c-fos和c-jun是神经系统内两种十分重要的癌基因，受到外来刺激或神经系统损伤时，可以有快速的表达，其编码的蛋白产物作为一种&核内第三信使&参与调节细胞的信号转导过程。

A reduction in actin expression was observed by immunoblotting in both nucleus pulpous and anulus fibrous cells, especially in nucleus pulpous cells.

Western免疫印迹结果显示，肌动蛋白在纤维环细胞和髓核细胞中的表达均明显减少，以髓核细胞为著。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐