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Objective To study relation of the expression of laminin to the degree of adenoid cystic carcinoma of the salivary gland.

目的 研究层粘连蛋白在涎腺腺样囊性癌中的表达特征及其与腺样囊性癌组织分化程度的关系。

in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要:为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法,根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物,进行多重pcr扩增,并对多重pcr反应体系进行优化。

Results Transient myocardial ischemia/reperfusion induced obvious accumulation of KEF 4 mRNA in rat. Oxidative stress distinctly raised the KLF 4 mRNA and protein levels in C2C12 cells.

结果 短暂缺血再灌注后,大鼠心肌组织中Krüppel样因子4mRNA的水平明显增加;过氧化氢处理C2C12肌原细胞后,Krüppel样因子4mRNA和蛋白的水平均明显增加。

The main results were as follows. The main nutrition bioactive components in Opuntia Milpa Alta were analyzed using chemical and instrumental analytical methods. The results showed that the content of crude protein and fat was 0.65%~0.68% and 0.30%~0.31% respectively. The total content of the amino acids was 6.81%~7.38%, and Glu was 1.08%~1.52%.The contents of pectin and cellulose was 1.47%~1.68%, 0.87%~1.22% respectively. They were oxalate free and rich in Ca 5.6%, Fe 8.06mg/100g, Zn 3.30mg/100g, VB6 5.62mg/100g, low in tannin 0.02%~0.04%.The bioactive matter content such as polysaccharide, polyphenol, and flavone were high, about 1.27%, 63.14mg/100g, 36.21mg/100g respectively.

主要研究内容如下:采用化学和仪器分析方法,对米邦塔仙人掌中的主要营养和生理活性成分进行分析,结果表明,粗蛋白含量为0.65%~0.68%,脂肪含量为0.30%~0.31%;氨基酸总量为6.81%~7.38%,其中谷氨酸含量达1.08%~1.52%;果胶、膳食纤维分别为1.47%~1.68%和0.87%~1.22%;干样钙含量5.6%,铁8.06mg/100g,锌3.30mg/100g;B族维生素丰富,其中干样VB6含量5.62mg/100g;单宁0.02%~0.04%;不含草酸;生理活性物质如粗多糖、多酚、黄酮类物质分别约为1.27%、63.14mg/100g、36.21mg/100g,半乳糖约占多糖中单糖总量的80%;HPLC检测出8种酚类物质,绿原酸含量最多达42.40μg/g,其次是芦丁和槲皮素,其含量分别为25.02μg/g和10.91μg/g。

Anthropometrical measurements were performed and serum concentrations of IGF-I, insulin-like growth factor binding protein-3 (IGFBP-3), leptin, soluble leptin receptor, as well as the estimated molar excess of sOB-R over leptin were obtained. Two groups were formed.

执行人体测量,获得了胰岛素样生长因子-1、胰岛素样生长因子连接蛋白-3(IGFBP-3)、瘦素、可溶性瘦素受体的血清浓度和可溶性瘦素受体超过瘦素的估计摩尔量。

Anthropometrical measurements were performed and serum concentrations of IGF-I, insulin-like growth factor binding protein-3 (IGFBP-3), leptin, soluble leptin receptor, as well as the estimated molar excess of sOB-R oer leptin were obtained. Two groups were formed.

执行人体测量,获得了胰岛素样生长因子-1、胰岛素样生长因子连接蛋白-3(IGFBP-3)、瘦素、可溶性瘦素受体的血清浓度和可溶性瘦素受体超过瘦素的估计摩尔量。

The role of Serum amyloid A,matrix metalloproteinase,creactiveproteinand some other biological mark are regulated by inflammatory or autogenetic inhibition,which in diagnosing and curing ankylosing spondylitis are more and more clear.

血清淀粉样蛋白A、基质金属蛋白酶、C反应蛋白等生物标记物通过炎性因子或其自身抑制物等多种炎性物质调节在机体内的表达,作用机制在诊断和治疗强直性脊柱炎过程中已愈发明晰。

Methods Keratinocytes were isolated from the foreskins of children, and subjected to primary culture. The cultured keratinocytes were incubated with anti- keratin 16 antibody, or with isotype antibody as control, for various durations.

将抗角蛋白16抗体加入培养的角质形成细胞中,以同种型抗体作对照,研究外皮蛋白和Toll样受体2和4 mRNA表达。

Objective: To confirm the nucleolar localization of telomerase-regulation associated protein-human N-acetyltransferase-like protein and its associated functions.

目的:探讨端粒酶调节相关蛋白-乙酰转移酶样蛋白核仁定位的分子基础及相关功能。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18,mChIL-18)的cDNA基因序列,设计一对特异性引物,应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因,对扩增片段进行T-A克隆,经PCR、酶切鉴定及测序验证,成功获得ChIL-18成熟蛋白完整基因的克隆。

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