蛋白样的

Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562，采用MTT法检测细胞增殖活性，单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象，Annexin V FITC/PI双标法检测细胞凋亡，Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。

Radio-ligand binding assay was applied to determine the maximum binding and affinity changes of nicotinic acetylcholine receptor on five weeks old male Sprague-Dawley rat's lateral pterygoid muscle and superficial masseter muscle after the function mandibular advancement.

本研究在以往研究的基础上，首次对功能矫形的神经肌肉调控机理进行探索。应用放射受体分析法，从膜受体蛋白分子水平定量研究功能矫形前伸青春期SD大鼠（5周龄）下颌后，下颌前伸肌细胞膜烟碱样乙酰胆碱受体最大结合容量和亲和性的变化。

YIQIHUOXUEFANG can relieve the myelin sheathe swollen of the nerves and the disease of the sciatics, and promote local vessel density around the myelin sheathConclusionsYIQIHUOXUEFANG can regulate blood glucose effectively, improve the mico-circulation around the sciatics of the diabetes rats, restrain the metabolism of the multi-alcohol, increase the expressions of the NGFand MBP, i.e.

益气活血方能有效地调节血糖，改善DM大鼠坐骨神经周围微循环，抑制多元醇代谢，有醛糖还原酶抑制剂样作用，升高Na~+-K~+-ATP酶活性，改善和提高神经传导功能，促进坐骨神经神经生长因子和碱性髓鞘蛋白的表达，可明显缓解DM大鼠周围神经的早期病变，有一定的神经修复作用，综合疗效优于弥可保。

Gray matter damage was assessed on the basis of the number of normal neurons in the ventral horn. White matter damage was assessed on the basis of the extent of vacuolation and amyloid precursor protein immunoreactivity in the ventral and ventrolateral white matter.

根据腹角内正常神经元的数目评估灰质损伤程度，而白质损伤则根据空泡形成的程度以及腹内和腹外侧白质的淀粉样前质蛋白免疫原性来评估。

In order to study the effect of vascular damage in vivo during PDT, the chicken chorioallantoic membrane tumor model by using stable high GFP-expressing human adenoid cystic carcinoma cell strain is built for the experiment.

为了在活体肿瘤模型上实时监测PDT导致的血管损伤效应，使用稳定高表达绿色荧光蛋白的人涎腺腺样囊性癌细胞株，建立了基于鸡胚尿囊膜的肿瘤模型。

The high rates of metabolism and nutrient utilization in the gut are directly linked to the high rates of proliferation, protein secretion, apoptosis and desquamation of various epithelial and lymphoid cells within the mucosa, all of which are a key feature of the post-weaning period.

消化道新陈代谢和营养利用率高，直接与消化道高的增殖率，蛋白分泌，细胞死亡，不同上皮细胞的脱落和黏膜内淋巴样细胞相关，所有这些都是断奶后的一个主要特征。

PartⅡ:Effect of bicyclol on hepatocyte apoptosis in alcohol-intoxicated mice and related mechanismsThree consecutive intragastric administration of alcohol(6g/kg)in 12h interval resulted in an obvious liver injury and inflammation in mice,as indicated by the elevation of serum alanine transaminase(2.2 fold of control),increased hepatic COX-2 protein expression(2.2 fold of control),as well as liver pathological changes characterized by myelonic degeneration,vacuolar degeneration and inflammatory infiltration.

双环醇对酒精中毒小鼠肝脏细胞凋亡的保护作用及机制研究小鼠连续三次酒精(6g/kg)灌胃后可导致明显肝损伤，变现为血清ALT水平升高(为对照组的2.2倍)，肝脏炎症相关蛋白环氧合酶-2(cyclooxygenase-2，COX-2)表达增加(为对照组的2.2倍)，肝组织出现髓样变性、小空泡变性和炎性细胞浸润。

Objective To investigate the mechanism of neurocytes' damage caused by Aβ25-35 and the change of enzyme in the signal transduction passway.

目的 探讨β淀粉样蛋白25-35 (Aβ25-35)导致神经细胞损伤的机制及在损伤过程中涉及的信号转导通路中相关酶caspase-3的变化。

In lung cancer tissues, benign phymatoid lesions, and adjacent non cancer lung tissues, telomerase activity was determined by telomeric repeat amplification protocol assay, and the expression of human telomerase RNA component, human telomerase reverse transcriptase, and human telomerase associated protein(hTEP1) mRNA were detected with reverse transcriptase PCR. The results were compared with the histological types, degree of differentiation, and TNM stage of lung cancer.

用 TRAP法检测端粒酶活性，用逆转录 PCR法检测肺癌组织、肺部良性瘤样病变和病灶旁非癌肺组织的 RNA成分（ human telomerase RNA component，hTR）、端粒酶逆转录酶（ human telomerase reverse transcriptase， hTERT）、端粒酶相关蛋白 1（ human telomerase associated protein， hTEP1）各亚基的 mRNA表达，其结果与肺癌的组织类型、分化程度及 TNM分期进行了比较。

In order to grope for the new idea and new approach of aspartame synthesis,strains for hydrolyzing aspartame were screened from soil.

为了探索酶法合成阿斯巴甜的新思路和新方法，从富含蛋白的土样中筛选出能够分解二肽阿斯巴甜的菌种。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。