蛋白样的

Objective To explore the effect of hyperphosphorylated tau protein on the formation of brain amyloid.

目的 研究tau蛋白过度磷酸化对脑淀粉样蛋白生成的影响。

The method uses radiative immunity analysis, determine 43 senile domentia and 33 hemal sexes are gawkish (the β in VD) patient and plasma of 58 healthy old people - the content; analysis of amylaceous appearance albumen and β of observation AD patient - the variable relation of the content of amylaceous appearance albumen and GDS process is reached and treat from beginning to end.

方法应用放射免疫分析，测定43例老年性痴呆和33例血管性痴呆患者及58例健康老年人血浆中的β-淀粉样蛋白的含量；分析和观察AD患者β-淀粉样蛋白的含量和GDS进程的变量关系及和治疗前后β-淀粉样蛋白含量的变化。

The expressed apoE protein in central neuronal system functions potentially in deposition and clearance of amyloidal peptide, sustaining the stabilization of microtubule proteins, cellular signal transduction, immune regulation, glucose metabolism and oxidation stress.

中枢神经系统中的apoE对脑组织淀粉样蛋白的沉积与清除、微管蛋白稳定性的维持、细胞信号转导、免疫调节、葡萄糖代谢、氧化应激等都有潜在的作用，apoE在体内发挥十分重要的生物学活性。

A total of 90 healthy male Sprague Dawley rats were selected for this study. Twelve rats were used for the sham operation group. Left anterior descending coronary artery of the remaining rats was deligated to establish models of myocardial infarction. After 24 hours of deligation, 36 rats were randomly divided into the control group and experimental group, 18 rat in each group.

目的：检测人胰岛素样生长因子Ⅰ基因修饰的鼠骨骼肌成肌细胞移植后，心肌梗死大鼠血浆及心肌组织内胰岛素样生长因子Ⅰ蛋白浓度的变化，以及心肌细胞内胰岛素样生长因子Ⅰ和端粒酶反转录酶mRNA水平的表达。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Unlike previous models in which overexpression of the amyloid precursor protein results in amyloid plaque formation, have produced mice that overexpress only Aβ40 or Aβ42 and prove that Aβ42 is critical for the formation of amyloid deposits in vivo.

不同于早先调控淀粉样蛋白前体蛋白的动物模型，McGowan 等(Neuron， Vol 47， 191-199， 21 July 2005)培养了专一表达Aβ40或者 Aβ42的老鼠，结果直接证明了Aβ42 确实是引起淀粉样蛋白沉淀的主要因素，而不是Aβ40。

In serial passaging experiments, the genome of the chimeric pHBIV-2 could exist stably. The transcription was active and the activated proteins could be detected.

3在感染传代细胞中，嵌合克隆pHBIV-2基因片段可长期稳定地存在，转录始终处于活跃的状态；蛋白水平亦可检测到有功能性的蛋白；电镜结果也证实了培养上清中存在类似于HIV样的病毒颗粒。

The membrane protein gene of Infectious Bronchitis Virus like was obtained from alpacas for the first time. And it was cloned into the pMD-18T vector.

本试验首次从羊驼体内获取了禽传染性支气管炎样病毒的膜蛋白基因，并克隆到了pMD-18T载体中，获得了膜蛋白基因的克隆质粒。

Methods Expression of the FHIT and EGF was detected by immunohistochemical analysis in 20 normal vulvas and 22 vulvar intraepithelial neoplasias as well as 60 primary vulvar carcinomas.

用免疫组化SP法对20例正常外阴组织，22例外阴上皮内瘤样病变以及60例外阴癌组织分别进行FHIT蛋白和EGF蛋白表达的检测。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。