蛋白固定的

Methods Colloidal gold-labeled monoclonal antiCK20 antibody was absorbed on nitrate cellulose membrane, and then the membrane was fixed on plastic strip with double face glue as checking line.

方法将经处理的CK20单抗标记在胶体金颗粒上并活性进行鉴定，合格后吸附在硝酸纤维膜上并固定在长方形硬质塑料胶片一端为测定线；同时将吸附有IgG蛋白胶体金的硝酸纤维膜固定在测定线的一侧为质控线。

The test results showed that the amount of this kind of function material to conjugate proteins were larger than 1.5ig/mm2and its one time adsorption rate was larger than 80% and can satisfy the requirements for solid-phase radioimmunoassay. Test results showed that this polymer-NHS-ester can connect with proteins through covalent bounds under mild conditions This technical method will supply a type of new material for solid-phase radioimmunoassay.

实验证实，这类聚合物NHS酯可在温和条件下与蛋白反应，使蛋白以共价键结合方式固定在聚合物上，这种功能材料对蛋白的结合量大于1.5μ／mm~2，对蛋白的一次吸附率大于80％，能满足固相放免分析的要求，将为固相放免分析提供一种新材料。

The PAZ domain is an RNA-binding module found in Argonaute and some Dicer proteins and its structure has been determined in the free state.

现在，结构研究确定，PAZ （Argonaute蛋白家族特有的一个区域，该家族的蛋白与RNA沉寂机制有关）专门识别siRNA，方法是通过将siRNA的3个悬臂固定。

With polystyrene functional fluorescent microspheres as the matrix, the immobilization of serum protein and trypsin, and the activity of immobilizing trypsin have been studied.

分别考察了不同条件下，聚苯乙烯功能荧光微球对血清蛋白以及胰蛋白酶的固定化，对固定化胰蛋白酶的活性进行了研究。

The hydrolysis of soybean protein isolate with immobilized papain for making soy peptide was studied.

采用固定化木瓜蛋白酶水解大豆分离蛋白，对大豆分离蛋白的最佳预处理温度进行了探讨，并对制备大豆肽的工艺条件进行了正交实验。

A comparison study of WSC (water-soluble carbodiimide) conjugation method and entrapment method for PDLLA surface modification via silk fibroin is performed in this work.

利用WSC法和内陷法以丝素蛋白修饰聚乳酸膜的对比研究表明，后者较前者能更有效地固定丝素蛋白，也更能提高改性膜与细胞的亲和性

Methods:Animal models of 25 adult mixed breed dogs were established by transverse osteotomy at bilateral iliopectineal crest,which were fixed with ATMFS anterior column connector and steel plate respectively. The animals were examined at 2,4,6,8,and 12 weeks by X-ray,macropathological observation and biomechanical test. Tetracin fluorescent labeling,HE staining,Masson triad colour staining,in situ hybridization and immunohistochemical method were performed and histological image analysis were applied.

建立犬双侧骨盆弓状线骨折的动物模型，分别采用ATMFS前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周分别行影像学检查、大体观察及生物力学测试；采用四环素荧光标记法、HE染色、Masson三色染色法、原位杂交法、免疫组织化学法进行组织学观察并图像分析；采用RT-PCR法检测骨折端不同时期骨钙蛋白和核心转录因子mRNA相对表达量的变化。

It is for the first time that Spinacia oleracea ferredoxin immobilized on the surface of the carbon nanotube modified glassy carbon electrode(SoFd-CNT/GC) can undergo effective and stable direct electron transfer reaction.

将来源于Spinacia oleracea的铁氧化还原蛋白(ferredoxin，SoFd)通过吸附的方法固定在碳纳米管表面。红外光谱表明SoFd固定在CNT表面后没有变性。

To obtain a basal concentration of serum amyloid A in cattle in Taiwan, SAA concentrations were determined from serum samples collected from 10 healthy heifers, once a week for 10 weeks.

为测定台湾牛只急性期蛋白血清淀粉样蛋白A (serum amyloid A， SAA)的血清浓度，本实验采集血清的对象为10头健康女牛，每周固定时间采样一次，共10周。

The mechanism, properties of this immobilization method and response characteristics, application probables for potentiometric and amperometric enzyme-based sensors have been investigated and discussed.

以脲酶和葡萄糖氧化酶为研究对象，首次利用活蚕液状丝素蛋白的构象变化即蛋白质的变性现象，将酶固定于蚕丝素蛋白中，提出了这种固定化方法的机理、性能以及用作电位法和电流法酶传感器响应特性的研究和实际应用的可能性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。