蛋白固定

The coverslip with differentiated cells were fixed at different timepoints and used for toludine blue or inmunohistochemical dying Meanwhile, different doses of antibody to Fibronectin were added to observe aggregating phenomenon.

用2，3传代细胞按一定比例种植于24孔板内，加入TGF-β1 1，5，10ng／mL，分别于不同时间点取材固定，用于甲苯胺兰组织学染色及抗纤维连接蛋白抗体免疫组化染色。

Methods: A 6-mm unilateral femoral segmental defect was surgically created in 120 Sprague-Dawley rats, internally stabilized with a polyacetyl plate and 6 Kirschner wires, and contaminated with 104 colony-forming units of Staphylococcus aureus. After 2 weeks, all defects were surgically debrided and implanted with 0, 20, or 200 g of rhBMP-2 in a type 1 bovine collagen sponge.

通过外科手术在120只Sprague-Dawley大鼠一侧股骨制造了6毫米的缺损，内部通过聚乙烯板及克氏针固定，并被104个金黄色葡萄球菌菌落污染。2周后在所有的缺损处通过1型牛胶原海绵注入0、20或200g重组人骨生成蛋白。

The expression of VEGF, bFGF and BMP-2 were detected in femoral head through making use of immunohistochemistry and in-situ hybridization technique.Results The organization structure of experimental group was disorganized cracked and there were a great number of empty lacunas in bone trabecula.

所有标本取材后经固定、脱钙后，制成切片，光镜及电镜观察其病理变化及骨修复情况，并以免疫组化和原位杂交技术对其VEGF蛋白、bFGF与BMP-2mRNA的表达情况进行检测。

Objective In order to understand the mechanism of stimulative effects of compression plate on bone healing in the treatment of mandibular fracture.

目的 通过观察犬下颌骨骨折的不同内固定后不同时期骨断端两侧骨形成蛋白的表达，对加压促进骨愈合的机制进行初步探讨。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。