蛋白固定

in order to improve the anticoagulation of biomedical stainless steel, we fabricated the plasma-polymerized allylamine films on the surface of the biomedical stainless steel by pulsed plasma polymerization technique, and then immobilized the molecules of bovine serum albumin on the polyallylamine films.

采用脉冲射频等离子体聚合技术，在医用不锈钢表面制备了等离子体聚烯丙胺薄膜，并进一步在聚合薄膜表面固定白蛋白分子。

Otherwise, an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, the covalent binding of bioactive protein to the free terminal NH2 groups using glutaraldehyde as a coupling agent was followed.

并利用丙烯胺的电浆处理在钛金属表面键结上胺基(-NH2)的官能基，之后再以交链剂戊二醛来固定生物活性胶原蛋白於低温电浆处理后的钛金属表面。

The glutaraldehyde was coupled with amido group of chitosan, and template protein-bovine hemoglobin was immobilized by aldehyde group.

用壳聚糖包裹的硅胶为载体，利用壳聚糖表面的氨基与戊二醛结合，在硅胶表面引入醛基，固定模板蛋白。

Surface modification of albumin chitosan blended membranes have been reported by us, via immobilization of bioactive complexes or coupling biomolecules on liposome-modified membranes through carbodiimide functional moieties .

表面修饰白蛋白混纺壳聚糖膜已报道的时候，经固定化生物活性物或生物大分子耦合对脂质体膜修饰通过碳化功能材料。

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后，一部分取其中脑黑质节段，固定、包埋做连续冠状石蜡切片，以免疫组织化学染色方法，显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞，光镜观察并细胞计数，统计学分析；另一部分取其中脑黑质组织匀浆，行TH、CB的Westem blot免疫印迹方法检测，用LabWorks软件分析处理。

Prophenoloxidase is a key enzyme for mediating melanotic encapsulation of oocysts,which may be transformed into active phenoloxidase by limiting proteolysis.

而前酚氧化酶(Prophenoloxidase，PPO)是介导卵囊黑化包被反应的一种关键酶，活化后可催化酪氨酸羟基化为二羟基苯基丙氨酸，二羟基苯基丙氨酸和多巴胺可进一步氧化为具有活性的苯醌活性，苯醌即可介导蛋白交联并聚合成黑色素层固定、隔离或杀死入侵的病原体。

Results (1)Ostealgia is the most common symptom of light-chain MM(54.1%).

结果 (1)轻链型MM临床症状主要为骨痛，实验室检查可有贫血(73 9%)、骨骼损害(87%)、血钙增高(2 1 7%)、蛋白尿(10 0 %)、肾功能损害(4 3 5 %)、血清球蛋白水平降低，血清蛋白电泳多无阳性发现，免疫固定电泳可明确分型。

The IgG binding domain of Streptococcal Protein G which can selectively immobilizes the Fc regions of immunoglobulin G is a kind of good material for oriented immobilization of antibodies in antibody microarrays.

链球菌G蛋白的IgG结合域能够特异性地结合IgG 的Fc区，是制备免疫微阵列的一种理想的IgG固定材料。

The rats were orally treated with referential ferulaic acid, Rhizoma Chuanxiong extraction and Naodesheng capsule repectively. Blood samples were collected by cutting rats tails. Plasma was separated by centrifugation at 10 000 r·min-1 for 10 min, and two-times methanol in volume was added to deposit proteins. After centrifugation, the upper liquid was transferred to filter. The concentration of ferulaic acid in serum was determined by RP-HPLC.

大鼠分别灌胃给予阿魏酸对照品、川芎药材提取物、复方脑得生片，断尾采血，离心，取血浆适量，加甲醇沉淀蛋白，取上清液过滤，用HPLC分析，以C18为固定相，流动相为甲醇-0.5%醋酸水溶液（30∶70），在320 nm检测阿魏酸血药浓度，3p97软件处理数据。

Results: After cryofixation,basement membranes of skeletal muscle consisted of only one electron dense layer, T tubules were round, core cylinders were observed in terminal cisternae and there were thread-like protein particles on the membranes of terminal cisternae.

结果：冷冻固定后，缝匠肌基膜仅仅由电子密度高的一层组成；横小管为圆形；终池内含有电子密度高的环形物，终池膜上存在一排线状电子密度高的蛋白颗粒。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失