蛋白固定

In the end, the bioactivity and biocompatibility of samples were studied based on apatite precipitation and osteoblast culture in vitro.

最后，通过体外仿生矿化和成骨细胞培养来研究固定蛋白前后样品的表面生物活性和生物相容性。

Because the full length DFF45 recombinant protein was unstable in the solution, it means that either the DFF45 protein has uncompacted region in the structure or the protein has several domains.

由于细菌表达的全长DFF45重组蛋白表现出不稳定易降解的特点，说明该蛋白可能具有无固定结构的区域，或者含有多个结构域。

After that, all samples were fixed with 10 g/L paraform for 45 minutes. Then 50 μL well-fixed blood was added into the polystyrene tube, meanwhile 20 μL monoclonal antibody, such as CD42a-b-c-d and PE-labeled CD42b, was respectively mixed gently with the blood sample and incubated at room temperature in dark for 30 minutes.

取50 μL固定后全血加至流式检测管，同时分别向不同管中加入20 μL糖蛋白Ib/IX/V 复合物单克隆抗体CD42a-b-c-d和PE标记的糖蛋白Ibα单克隆抗体CD42b，混匀后室温避光孵育30 min，然后CD42a-b-c-d标记的样品中加入20 μL FITC标记的羊抗鼠 IgG，混匀后室温避光孵育30 min。

A comparison study of WSC (water-soluble carbodiimide) conjugation method and entrapment method for PDLLA surface modification via silk fibroin is performed in this work.

利用WSC法和内陷法以丝素蛋白修饰聚乳酸膜的对比研究表明，后者较前者能更有效地固定丝素蛋白，也更能提高改性膜与细胞的亲和性

MATERIALS AND METHODS: hMSCs labeled with enhanced green fluorescent protein were transplanted into the lateral ventricle of neonatal mouse brain. At 0, 9 and 14 days post-transplantation, MICE were sacrificed and their brains were fixed with 4% paraformaldehyde. The engraftment of transplanted cells in coronal section of the recipient mouse brain was examined wider a fluorescent microscope. Indirected immunofluorescent staining was used to detect the expression of neural proteins of these grafted cells.

材料与方法：将标记绿色荧光蛋白的人骨髓间充质于细胞植入到新生3d的小鼠侧脑室中，分别于移植后0d，9d和14d处死受休鼠，取其脑组织，4%多聚甲醛固定后行冠状面冰冻切片，荧光显微镜下观察hMSCs的植入，激光共聚焦显微镜检测植入细胞神经特异性蛋白的表达，定位双重标记的植入细胞。

Preliminary studies on affinity isolation based on the specific combination between Biotin and Avidin Presently, affinity chromatography is increasing to be a main protein isolation and purification technique for a complicated system because of its high specificity, high rate of recovery, etc.

利用生物素和抗生物素蛋白间的特异性结合作用，将生物素通过化学反应键合到蛋白质的某种官能团上，酶解后的肽段通过固定了抗生物素白蛋白的亲和分离凝胶色谱柱，可以选择性地分离生物素化的肽段，达到简化复杂体系的目的。

Second is studying the traumatic condition changes in a choosey pressure at different post-PT phases. Moreover, we detected model PLA_2 activity, express of PLA_2 subtype mRNA by fluorescent quantitative RT-PCR technique, protein express and site by Western blot and IHC at different post-PT phases. At last, we studied the PLA_2 inhibited effects and model protection of Variabilin in vivo.

研究在不同压强的外力作用下，模型指定时相点的一般情况和存活率、大体、光镜及电镜下胰腺组织的病理变化和常规酶学变化，得到稳定建模致伤压强；然后，固定致伤压强，研究胰腺创伤后不同时相点的伤情变化(包括一般情况、胰腺组织的病理变化、AMS和LPS活性变化、血常规、血生化、胰腺细胞凋亡以及胰腺组织细胞生长周期变化情况)；再次，采用3H-花生四烯酸标记大肠杆菌膜作为底物的方法检测大鼠胰腺创伤后不同时相点的PLA_2活性，利用荧光定量RT-PCR技术检测其亚型mRNA的表达，利用Western blot和IHC技术检测其蛋白表达量和蛋白组织定位；最后，将Variabilin体内应用于急性胰腺创伤大鼠模型，研究其对模型的保护作用和对PLA_2的抑制效应。

To obtain a basal concentration of serum amyloid A in cattle in Taiwan, SAA concentrations were determined from serum samples collected from 10 healthy heifers, once a week for 10 weeks.

为测定台湾牛只急性期蛋白血清淀粉样蛋白A (serum amyloid A， SAA)的血清浓度，本实验采集血清的对象为10头健康女牛，每周固定时间采样一次，共10周。

Coli. The protein was expressed in a soluble form and as a fusion protein. There was a 6 histidines tag at its amino acid terminus. It was purified by immobilized metal ion affinity chromatography under native conditions.

所表达的蛋白是一种可溶性的融合蛋白，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。

His current research interests include: biochemical studies of metallothionein-like metal binding proteins and phytochelatins in plants; immobilised algal biotechnology; and conservation strategies for mangroves and their application in sewage treatment.

目前他致力於研究以下项目：类金属硫蛋白的金属结合蛋白的生物化学以及植物的鳌剂研究；固定化微藻的生物工艺学研究；研究保护红树林策略以及利用红树林进行废水处理。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。