蛋白固定

Methods Colloidal gold-labeled monoclonal antiCK20 antibody was absorbed on nitrate cellulose membrane, and then the membrane was fixed on plastic strip with double face glue as checking line.

方法将经处理的CK20单抗标记在胶体金颗粒上并活性进行鉴定，合格后吸附在硝酸纤维膜上并固定在长方形硬质塑料胶片一端为测定线；同时将吸附有IgG蛋白胶体金的硝酸纤维膜固定在测定线的一侧为质控线。

This is due to the fact that AFP in the sample flows through the sample pad and binds the conjugate to form a complex. This complex then migrates down to the membrane and binds to the immobilized anti-AFP antibody A2 of the test line, resulting in the immobilization of the reddish conjugate.

如果样品中含有甲胎蛋白，膜上便会出现肉眼可见的微红色检测线，这是由于样品中的AFP与标记有单克隆抗体A1的免疫金结合形成复合物，复合物向膜上移动与固定在膜上的检测线单克隆抗体A2结合反应，结果在该处形成固定的微红色线条。

The PAZ domain is an RNA-binding module found in Argonaute and some Dicer proteins and its structure has been determined in the free state.

现在，结构研究确定，PAZ （Argonaute蛋白家族特有的一个区域，该家族的蛋白与RNA沉寂机制有关）专门识别siRNA，方法是通过将siRNA的3个悬臂固定。

With polystyrene functional fluorescent microspheres as the matrix, the immobilization of serum protein and trypsin, and the activity of immobilizing trypsin have been studied.

分别考察了不同条件下，聚苯乙烯功能荧光微球对血清蛋白以及胰蛋白酶的固定化，对固定化胰蛋白酶的活性进行了研究。

The hydrolysis of soybean protein isolate with immobilized papain for making soy peptide was studied.

采用固定化木瓜蛋白酶水解大豆分离蛋白，对大豆分离蛋白的最佳预处理温度进行了探讨，并对制备大豆肽的工艺条件进行了正交实验。

We also compared the plasma-treated ODT surface with that after AAc-grafting. Using similar method to immobilize collagen in wet cell system, both surfaces were capable to have collagen immobilized. Nevertheless, their immobilized quantities were significantly different. The most pronounced processes are the participation of oxygen to the plasma-treated ODT surface, the generated oxidative groups, particularly the limited amount of O=C-OH group, are likely to bind with protein molecules.

另一方面，将仅电浆处理及随之以丙烯酸接枝后的两种 ODT 表面以相同方式传送至真空湿室反应室中进行连续的蛋白质固定，经由 X 光光电子能谱分析后，发现两者仍可以固定胶原蛋白，但在定量程度上有差异，经推论为：仅电浆处理之 ODT/Au，Ag 试片受到氧的参与反应，故产生能接著蛋白质分子的氧化基团，其中包括有限量的 O=C-OH 基。

Methods:Animal models of 25 adult mixed breed dogs were established by transverse osteotomy at bilateral iliopectineal crest,which were fixed with ATMFS anterior column connector and steel plate respectively. The animals were examined at 2,4,6,8,and 12 weeks by X-ray,macropathological observation and biomechanical test. Tetracin fluorescent labeling,HE staining,Masson triad colour staining,in situ hybridization and immunohistochemical method were performed and histological image analysis were applied.

建立犬双侧骨盆弓状线骨折的动物模型，分别采用ATMFS前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周分别行影像学检查、大体观察及生物力学测试；采用四环素荧光标记法、HE染色、Masson三色染色法、原位杂交法、免疫组织化学法进行组织学观察并图像分析；采用RT-PCR法检测骨折端不同时期骨钙蛋白和核心转录因子mRNA相对表达量的变化。

It is for the first time that Spinacia oleracea ferredoxin immobilized on the surface of the carbon nanotube modified glassy carbon electrode(SoFd-CNT/GC) can undergo effective and stable direct electron transfer reaction.

将来源于Spinacia oleracea的铁氧化还原蛋白(ferredoxin，SoFd)通过吸附的方法固定在碳纳米管表面。红外光谱表明SoFd固定在CNT表面后没有变性。

Half-ring sulcated external fixator was used for the fixation.

专利号：CN01256565。方法：山羊24只，随机分为单纯异种脱蛋白骨组、自体骨组及异种脱蛋白骨＋重组人骨形态发生蛋白组，在每只山羊右侧胫骨中下段截除胫骨总长度20%形成节段性骨缺损，按分组情况植入不同材料，采用半环槽式外固定器固定。

Acceptable resolutions were achieved when it was applied for the separation of dipeptides including Leu-Tyr and Val-Tyr by using 1-butanol-acetic acid-water (4:1:5, V/V/V) solvent system. The proteins including cytochrome C and myoglobin, lysozyme and myoglobin, and fresh chicken egg-white proteins were well separated by 12.5% PEG1000-12.5% K2HPO4-75% water (pH 9.0) and 16% PEG 1000-12.5% K2HPO4-71.5% water (pH 8.0) system.

应用研究表明，采用正丁醇-醋酸-水(4：1：5， V/V/V)和PEG1000-磷酸钾盐-水(12.5：12.5：75， W/W/W)(pH 9.0)体系可以在较高的流动相流速和较高的固定相保留下分别实现对亮氨酸-酪氨酸和缬氨酸-酪氨酸二肽混合物以及细胞色素C与肌红蛋白、肌红蛋白与溶菌酶混合物及鸡蛋清样品中蛋白成分的成功分离。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。