蛋白固定

The test results showed that the amount of this kind of function material to conjugate proteins were larger than 1.5ig/mm2and its one time adsorption rate was larger than 80% and can satisfy the requirements for solid-phase radioimmunoassay. Test results showed that this polymer-NHS-ester can connect with proteins through covalent bounds under mild conditions This technical method will supply a type of new material for solid-phase radioimmunoassay.

实验证实，这类聚合物NHS酯可在温和条件下与蛋白反应，使蛋白以共价键结合方式固定在聚合物上，这种功能材料对蛋白的结合量大于1.5μ／mm~2，对蛋白的一次吸附率大于80％，能满足固相放免分析的要求，将为固相放免分析提供一种新材料。

To study the surface treatment of the quartz glass, and the coupling of the albumin protein, we used with the fictionalization of the copolymers, and experimental conditions for protein immobilization.

蛋白质固定化之制作程序系将白蛋白固定於涂有光阻剂共聚物之石英玻璃表面，然后检测蛋白质於280 nm波长附近的吸光特性，以评估蛋白质固定化的效果。

Pike protein consists of S1 and S2 subunit. S1 is head and S2 is rod-shape part, intermolecular effort can bind each other. S1 can bind with host cell epimembranal acceptor. S2 can fix integral Spike protein on the cellular membrane and mediate cell-fusion with virus and host cell. There are significant antigen determinant of virus in Spike protein, genic heterogenesis can effect viral pathogenicity. SARS-CoV Spike protein, which has 23 pieces larvaceous glycosylation sit, consists of 1256 amino acid. Its N-terminals and C-terminals is corresponding conservative.

蛋白是I型膜糖蛋白，介导病毒与宿主细胞膜上的受体结合并与宿主细胞膜相融合。S蛋白由S1和S2两个亚基组成。S1形成球状部分，S2形成棒状部分，两者之间通过分子间的作用力相互结合。S1能与宿主细胞上的受体结合，S2可把整个S蛋白固定在细胞膜上，并介导病毒包膜与宿主细胞膜融合。S蛋白上有重要的病毒抗原决定簇，S基因的突变会影响病毒的致病性。

The S 2p, C 1s, N 1s and O 1s spectra were typically analyzed. Experimental result has demonstrated that using MUA/Au, Ag as the substrates, collagen immobilization process in wet cell system or in atmospheric environment did not have qualitative or quantitative difference. Firstly, our well cell system is feasible to carry out on-line collagen immobilization process.

实验结果显示：具有羧基之 MUA /Au，Ag 於真空湿室反应室中及传统大气环境下固定胶原蛋白，试片在两种环境所固定的胶原蛋白结构，在定性及定量分析上无显著差异，先证实在本真空湿室反应室中进行连续的蛋白质固定程序具体可行。

Y.-T. Wu : Wet Cell System for on-line characterization of collagen immobilization Student Dissertation, Second price Winner , SRRC Eighth Users' Meeting and Workshop on Application of Synchrotron Radiation in Biology, Oct.

吴奕德91M01：利用真空湿式反应室进行即时就地胶原蛋白固定之研究。

The result of immobilization showed that hydrophile membranes were more advantageous than hydrophobe ones to act as immobilization carrier. Crosslinking organophosphorus hydrolase by glutaraldehyde on polyethersulfone membrane is a simple and practical immobilization method. An appropriate amount of bovine serum albumin and crosslinker is necessary to get a good result of immobilization. In the biodegradation of methyl-parathion through enzyme membrane reactor, the effect of immobilized enzyme amount, flow rate of peristaltic pump, pH of the feed, and the methyl-parathion concentration on biodegradation rate was studied. It was shown that the biodegradation rate increased with immobilized enzyme amount and substrate concentration. Biodegradation rate didn't increase when the folw rate of the peristaltic pump was larger than 7 ml/min.

然后，研究固定化酶降解甲基对硫磷，从不同材料的膜载体固定有机磷水解酶的比较中，发现亲水膜比疏水膜适合作固定化酶载体；在此基础上，将有机磷水解酶固定在聚醚砜微滤膜上，并制成酶膜生物反应器，用于降解甲基对硫磷；戊二醛化学交联法固定时，酶液与10%牛血清白蛋白溶液的比例在3：1~2：1，与交联剂的比例在7：2~7：4范围内，固定化效果较好，并发现牛血清白蛋白和膜载体有降低酶活损失的作用；将固定化的酶膜装于酶膜反应器降解甲基对硫磷，实验结果表明：反应器的降解速率与固定化酶量呈正比，也随底物浓度的增加而加快，当流速低时，降解速率随流速增大而加快，到7ml/min以上时，降解速率不随流速脑黾佣洌到獾淖畹团ǘ任？

Min~(-1) fed in pH of 7.0.(3) DCase was dialysed via pressure of 70MPa,time of 15min.

以TJS环氧基树脂作为固定载体，最适固定化条件为：TJS用量1g对应133u酶活，固定时间15h，温度28℃，pH值7.5最后固定化酶活为58u·g~(-1)，蛋白固定率可达98％，酶活回收率达到49.3％。

II. Exploring non-radioactivc method to select the optimal DNA-bindingsequencc fOr singIe-chain reprcssorCloning and expression of single-chain repressors with cysteine tai1 wereperformed and immobilized these protein in the solid surface by using the standardmicrowell plates Which have been activated to contain maleimide grouPs, al1owing tobind sulthydryl-containing protein on its surface.

二。非同位素的方法筛选单链阻遏蛋白的最佳DNA结合序列初探克隆和表达了带半眈氨酸尾的单链阻遏蛋白，利用己包彼了马来酚胺的活性板可以与自由琉基结合的特性，将蛋白固定在活性板表面。

The mechanism, properties of this immobilization method and response characteristics, application probables for potentiometric and amperometric enzyme-based sensors have been investigated and discussed.

以脲酶和葡萄糖氧化酶为研究对象，首次利用活蚕液状丝素蛋白的构象变化即蛋白质的变性现象，将酶固定于蚕丝素蛋白中，提出了这种固定化方法的机理、性能以及用作电位法和电流法酶传感器响应特性的研究和实际应用的可能性。

The expressed E2 protein was conjugated to ELISA microplate which was precoated with anti-E2 mAb H53. Then Raji cells were seeded onto the microplate and cultured .

将表达的E2蛋白固定在事先包被了E2单抗的酶标微孔板上，然后加入Burkitt淋巴瘤Raji细胞，可观察到该细胞聚集贴附生长。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。