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The carboxyhemoglobin levels of in-flowing pulmonary blood and out-going pulmonary blood were determined at 2,4 and 6 hours after treatments. Malondialdehyde contents and superoxide dismutase activities in the lung,liver and blood were also determined. Pathological changes in lung and liver were examined with light microscope,and immunohistochemical technique was used for analysis of heme oxygenase-1 (HO-1) protein expression and distribution in lung and liver.

各组分别在制模后2、4和6 h测定出入肺血中碳氧血红蛋白水平;肺、肝组织及血液中丙二醛含量及超氧化物歧化酶活性;光镜下观察肺、肝组织形态学改变;免疫组化分析血红素加氧酶-1(HO-1)在肺、肝组织中的蛋白表达和分布。

Ribusco-1,5-bisphosphate carboxylase/oxygenase located in the chloroplast is the most abundant protein in the leaves of light-grown plants.

1,5-二磷酸核酮糖羧化酶/加氧酶位于植物叶绿体中,是绿色植物中含量最丰富的蛋白,它催化CO2固定和光呼吸作用的第一步。

Methods: Forty-five SD rats were divided equally into SMT group, cervical intervertebral disc cataplasia model group and normal control group. Three months later, the morphologic changes of intervertebral disc were observed to determine the proteoglycan contents and the apoptosis rate of the intervertebral disc cells.

选取8月龄雌性SD大鼠45只,随机分为实验组、实验对照组和正常对照组,实验组和实验对照组分别造颈椎静动力平衡失调模型,实验组腹腔注射SMT.3个月后光镜下观察椎间盘形态,测定蛋白多糖含量及椎间盘细胞凋亡率,并比较。

Methods Salley′s leukoplakia carcinogenesis model of golden hamster cheek pouch is used in this study. The results are analyzed correlatively among pathological chassification, prepared-chinese-ink injection and α-smooth muscle actin immunohistochemistry.

材料与方法采用Salley法诱导金地鼠颊囊白斑癌变,以光镜下病理观察诊断分级和墨汁灌注、甲平滑肌肌动蛋白免疫组化等与血管生成有关的检测结果作相关性分析。

Objective: To compare the PDT and light photosensitive effect between Phycobiliprotein Chromophore peptides and PhotofrinⅡ.

目的:比较藻胆蛋白色素肽与photofrinⅡ的光动力学疗法效果与日光光敏作用。

Western-blot and immunohistochemical assay methods were employed to detect the expression of FHIT. Apoptosis and cell cycle of cell were analyzed by FCMS. Morphological changes were observed by compound and electron microscope.

然后用流式细胞仪,普通光镜及透射电镜对获得稳定FHIT蛋白表达的转染组细胞和空真核表达质粒转染组细胞及未转染的亲本细胞凋亡的状况进行了考察,另外还通过流式细胞仪检测了三组浙江大学硕士学位论文细胞生长周期的惰况。

Methods: We divide the rats into 5 groups in random, with Morris method, detect the change of some active oxygen radicals such as super-oxide demitasse, Nitric Oxide Synthase, malondialdehyde etc. Also, we detect the change of cholinesterase, Monoamine Oxidize; using HE,PAS staining, immuno-histochemical technique, detect hippocampus CA1 cerebral pathology slices optical density value of NOS, CHE and lipofuscin of neural cell; detecting apoptosis rate and the expression of P53 , which are neuronal apoptosis related genes.

大鼠随机分为5组,采用"Morris水迷宫"法观察脑缺血性模型大鼠学习记忆功能,检测超氧化物歧化酶、一氧化氮合酶、丙二醛等自由基代谢异常的改变,检测胆碱酯酶、单胺氧化酶等老化相关酶的变化、脑组织神经细胞免疫组织化学技术、检查海马CA1区脑组织的病理切片,检测NOS、CHE光密度值;流式细胞技术:检测细胞凋亡率,及促细胞凋亡基因、P_(53)蛋白表达;病理切片采用HE染色、PAS染色及免疫组化法检查,检测对大鼠海马神经元细胞一般病理变化及脑细胞内脂褐含量。

The muscle samples were taken and check under microscopy and electro-microscopy,Desmin was checked with imunohistochemistry technique,maximal isometric titanic force,CK and LDH in blood,MDA and SOD in muscle were assessed for rats,before and after exercises to show muscle damage,maximal isometric torque,muscle soreness, circumference of the arm,range of motion of the elbow were assessed before and after exercise to show muscle damage,maximal eccentric torque, optimal angle of the elbow,total work during exercise were also assessed for college students.

观察指标包括:肌肉活检光镜及电镜观察,肌肉骨架蛋白Desmin组织化学荧光标记,电刺激最大强直肌量,血清LDH、CK及肌肉MDA、SOD等;等长峰力矩MIT,肌肉酸痛度SOR,肢体围度CIR,关节活动范围ROM,运动中离心峰力矩及其对应关节角度、所做总功等。

On creating model of cultured hippocampal neurons injured by amyloid, we studied the mechanisms of close interrelation between amyloid and Alzheimer"s disease at cell and molecular level with advanced test methods. For example, the technique of optical lens and electron microscope , immunohistochemical method,biochemistry.molecular biology and HPLC, so as to deeply probe into the mechanisms that Decotion of Rehamanniae prelects and treats Alzheimer"s disease.

本课题通过建立β淀粉蛋白损伤离体培养的海马神经元模型,运用光、电镜技术及免疫组化、生物化学、高效液相及分子生物学等先进的检测手段和方法,从细胞及分子水平进一步探索了Aβ与老年性痴呆发生、发展密切相关的综合机制,从而全面深入地探讨了地黄饮子防治AD的作用机理。

This change in light intensity represents albumin extravasation.

这些光强度的变化代表白蛋白的外渗。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。