蛋白光

QTY06 significantly decreased MUC5ac expression in trachea and bronchiole epithelium, and reduced the optical density and mucins area as detected by image analysis in rats with chronic airway inflammation.

QTY06使气管和细支气管上皮内黏蛋白MUC5ac表达的阳性染色区域和程度明显减轻，通过图像分析系统测得的光密度值和黏蛋白面积%均明显低于模型组。

Among the arylazides, fluorinated aryl azides are exceptionally promising because upon photoactivation, they lead to much more efficient photolabeling than non-fluorinated arylazides.

目前，尽管已经有一些磷脂光敏探针用于膜蛋白研究的报道，但是还没有使用以上两种芳基叠氮基团作为光敏基团的报道，在磷脂的极性头基上引入光敏基团的报道也极少见。

Objective After Intense pulsed light irradiation cavia cobaya skin, to evaluate the histological change of skin with HE stains; to evaluate the collagen fiber change with nitroxanthic acid stains; to quantitative analysis of hydroxyproline content. Confirm mechanism of action for Intense pulsed light on sun-damaged human skin.

目的 用强脉冲光照射豚鼠背部皮肤后，通过HE染色观察皮肤组织学的改变；用苦味酸染色观察皮肤胶原纤维的改变；定量分析豚鼠皮肤羟脯氨酸的含量，观察皮肤胶原蛋白的变化，进一步研究强脉冲光治疗皮肤光老化的作用机制。

Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用，并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1)，另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死，取出损伤节段脊髓用来组织病理学检查，同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过，到损伤后28天，我们观察发现，损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18，group 1∶9.89±1.29；p.05)，光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

Results Among the three groups,the children's rib cartilage had the most blood vessels,the most chondrocytes,well-distributed stain of matrixes,and the type Ⅱ collagen was expressed actively and highest in photedensity.The rib cartilage of teenager group had less blood vessels,unhomogeny distributed stain of matrixes,the enlarged and separated cartilage lacunas.The rib cartilage in adult group showed the least blood vessels,the least chondrocytes.the hyalinization of perichondium,the most deposition of calcium salt,and the type II collagen was expressed at the lowest level in photodensity.

结果 儿童组肋软骨膜血管最丰富，软骨基质染色均匀，软骨细胞数目最多，Ⅱ型胶原蛋白表达最活跃，平均积分光密度值最高；青少年组软骨膜内血管减少，软骨基质染色出现明显的不均质状，软骨陷窝体积变大，并呈分隔状，陷窝内软骨细胞数目减少，II型胶原蛋白表达较儿童组减弱；成人组软骨膜血管、细胞成分明显减少，软骨膜内的纤维成分明显玻璃样变，钙盐沉积较青少年组时明显增多，Ⅱ型胶原蛋白表达较青少年组减弱。

Compared to the control, the increment in membrane permeability, MDA and Pro contents were smaller, and the reducement in activities of SOD and POD, contents in soluble protein, dry weight of aboveground, dry weight of underground, plant height and stem diameter were smaller in leaves of pepper under low temperature and poor light stress with PEG seed-soaking. These results suggested that PEG seed-soaking could increase the resistance ability to low temperature and poor light stress of pepper seedlings.

PEG浸种处理可使辣椒幼苗地上部干质量、地下部干质量、株高、茎粗和叶片SOD、POD活性、可溶性蛋白含量的下降幅度均较低温弱光对照显著减缓，细胞膜透性、MDA与Pro含量的增加幅度均较低温弱光对照显著降低，表明PEG浸种处理能够提高辣椒幼苗对低温弱光的适应能力。

Establishing the atherosclerotic model of rabbits by feeding them with cholesterol, we measured their body weight, TC, TG and LDL. Drawing the materials from rabbit aortic wall and embedding them with paraffin to make into section preparation, we detected type Ⅰ collagen, NF-κB, VCAM-1, CD36, type Ⅰ collagen mRNA and NF-κB mRNA with immunohistochemistry and in situ hybridization technique and by means of color image analyzer .

通过高脂饲料建立兔AS模型，测定体重及血脂成分的改变；取其主动脉壁，石蜡包埋制成切片，采用HE、免疫组化及原位杂交法染色，光镜观察AS病变，并应用CMIAS真彩色医学图像分析系统，检测Ⅰ型胶原mRNA及其蛋白、NF-κBmRNA及其蛋白、VCAM-1及CD36蛋白表达定量A值，与对照组进行比较。

Some similar responses were observed in the both species.(1) After saturating illumination for 3 h, the original fluorescence Fo increased while the PSⅡ photochemical efficiency Fv/Fm declined significantly, but these parameters could largely recover to the levels of dark-adapted leaves after 3 h of subsequent dark recovery.(2) No net loss of the D1 proteins occurred after the saturating illumination.

一方面，大豆和南瓜叶片在饱和光下产生一些相同的变化：（1）经过3小时饱和光处理，它们的初始叶绿素荧光参数Fo都明显升高，而PSⅡ光化学效率Fv／Fm都明显降低，但经过随后的3小时暗恢复后，Fo和Fv／Fm都可以恢复到初始暗对照的水平；（2）在饱和光处理和随后的暗恢复过程中都没有发生D1蛋白的净损失。

This research take late-maturing variety adzuki bean Jihong-4 as the experiment material, After the photoperiod inducement at different leaf ages treatment, the result showed that the activity of NR under LF treatment is higher than CK treatment on the whole, and is lower than SD treatment; The content of the dissociation amino acid under these three treatments show a descend trend along with the leaf ages accretion, but the inducement increasing the content of dissociation amino acid, different leaf ages leaves show some difference, and alternating rise with SD treatment; Inducement can reduce the content of soluble sugar, and continuous inducement shows a accumulation effect; 2LF is the most sensitivity stage for soluble protein under photoperiodic inducement, From this stage, each leaf indumenta can reducing the content soluble protein, but continuous inducement reducing rising it.

本研究通过光周期诱导的方法，对中晚熟品种冀红4号红小豆进行不同叶龄进行处理，结果表明：所有叶龄处理下硝酸还原酶活性基本高于自然光处理而低于连续短日照处理下的；随着叶龄增大，各处理游离氨基酸总量均呈下降趋势，但光周期诱导可提高游离氨基酸含量，不同叶龄表现有所差异，与连续诱导交替上升；诱导可降低可溶性糖的含量，并连续诱导出现累积效应；2LF是光周期诱对可溶性蛋变化最敏感的时期，这个时期起单个叶龄诱导可降低可溶性蛋白含量，但连续诱导却可提高；LF处理下的类胡萝素总体含量高于另两个处理，而LF处理下的叶绿素含量在仅3叶龄前高于CK处理下的，随后出现下降趋势。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失