蛋白光

Groups were divided as follows: diabetic group, 1,25-2D3 treated group [1,25-2D3 group] and control group. At the 12 week, haematuria and proteinuria were determined and the kidneys were removed by histopathology, immunostaining, and the expression of Nephrin mRNA and protein in renal cortex were detected by reversed transcription-polymerase chain reaction.

建立糖尿病大鼠模型，实验12周末观察各组血尿、蛋白尿、肾组织光镜电镜、免疫组织化学，采用反转录聚合酶链反应检测其肾皮质Nephrin mRNA及蛋白的表达。

In this study, I found that Hu-DMP-1-GFP-2.783K-36 can drive the expression of the reporter gene in lymph node, palm, hair follicle, maxilla, mandible, and tail at the stage of 15.5 dpc.

本研究发现，此结构体可以驱动绿色萤光蛋白在胚胎15.5天小鼠的手臂、脚掌、毛囊、上颚骨、下颚骨和尾巴表现。

objective:to investigate the expression and significance of rb in the occurrence, development and regression of infantile hemangiomas.methods:the expression of rb was examined in the proliferative stage and catagen of human hemangiomas and normal skin tissues by using immunohistochemical technique.immunohistochemical technique for factorⅷ-related antigen was used to prove that the cells which expressed rb were endothelium.image analysis system was applied to measure the expression level of rb at different stages of hemangiomas and in normal skin tissues.results:the expression of rb was significantly lower in proliferating hemangiomas than that in involuting hemangomas(p.05).conclusion:rb might play an important role in the regression of human hemangioma endothelial cells and anti-angiogenesis.

目的：探讨rb蛋白在血管瘤发生、发展及退化过程中的表达状况及其意义。方法：采用免疫组织化学方法检测人皮肤血管瘤增生期、退化期及正常皮肤组织中rb的表达水平，并结合第ⅷ因子相关抗原的免疫组织化学染色证实表达rb的细胞是血管内皮细胞。利用计算机图像分析技术测量不同时期血管瘤组织和正常皮肤组织rb表达的积分光密度和面积。结果：增生期血管瘤内皮细胞rb表达水平低于退化期，差异有显著性（p.05），退化期血管瘤内皮细胞rb表达水平与正常皮肤组织相比，差异有显著性（p.05）。结论：rb通过抑制血管瘤内皮细胞增殖和血管生成而在血管瘤的退化过程中起重要作用。

The expression of VEGF, bFGF and BMP-2 were detected in femoral head through making use of immunohistochemistry and in-situ hybridization technique.Results The organization structure of experimental group was disorganized cracked and there were a great number of empty lacunas in bone trabecula.

所有标本取材后经固定、脱钙后，制成切片，光镜及电镜观察其病理变化及骨修复情况，并以免疫组化和原位杂交技术对其VEGF蛋白、bFGF与BMP-2mRNA的表达情况进行检测。

Therefore, we used a computer-based high throughput screening method to screen drug library to identify the possible binding drugs, thus increasing the added value for nsLTPs in drug delivery systems.

我们利用了电脑助药物筛选的技术来进行筛选，试找出可能与此一蛋白结合的药物，并结合萤光光谱的方法加以验证。如此，可进一步提高此一蛋白质的附加价值。

Objective To study the effect exerted by sodium dodecyl sulfate on the resonance light-scattering based on histone and DNA complex and to explore the orderliness of interaction in the three compounds.

目的 研究十二烷基磺酸钠对组蛋白与DNA共振光散射强度的影响，探讨三者相互作用规律。

B. The positive expression of E-cadherin protein was mainly located in membrane of liver cell in para-carcinoma liver tissue, showed yellow brown granules, expression in cytoplasm and nucleus were rare.

经统计学分析，β-catenin蛋白的阳性表达强度存在组间差异，即肝癌组的平均光密度高于癌旁肝组织组，差异有显著性意义(P.05)。

Vitami function as photoreceptor mechanism of retina, maintenance of integrity of epithelia, lysosome stability, glycoprotein synthesis.

它的作用有：视网膜光受体机制、维护上皮细胞的完整性、溶酶体的稳定性、糖蛋白的合成等。

Rresults from those studies can be summariazed as follow:(1) A preliminary study no correlationship between the UV-VIS reflection spectrum of tomato fruit surface and the lycopene amount of tomato fruit was undertaken. Based on observation from this study, a damage-free and in-situ detection technique of lycopene amount from tomato was developed with UV-VIS reflection spectroscopy;(2) The geometrical isomers of lycopene were separated successfully on C30-HPLC-PDA-ELSD. The absorption coefficients of Z-isomers were consequently dedermined. The geometrical isomer composition of lycopene could therefore be assessed. Those efforts formed a solid base to identify the natural product from synthetic compound of lycopene;(3) Lycopene resource from the fruit of Autumn oliver was proved to be available for industrial application. An extraction methos of lycopene from the fruit of Autumn oliver by supercritical carbon dioxide was developed at laboratory scale;(4) A method to extract lycopene by supercritical liquid with improved efficiency was developed; Data from this investigation suggested that a proper raw material pre-extraction process was important for a better extraction efficiency;(5) Variation in the geometrical isomer composition of lycopene during its metabolish in rat was assessed. A large amount of Z-isomers were observed. This variation took place in serum;(6) The absorption rate and accumulation in serum of natural lycopene in rat were assessed;(7) Ability to quench singlet oxygen by different geometrical isomers of lycopene was compared;(8) It was detected that whether natural lycopene had functions to regule blood-lipids and LDL antioxidation;(9) A C31 degradation piece of lycopene was found in the prostate gland of rat.

研究结果包括：[1]应用UV-VIS反射光谱法建立了番茄果实中番茄红素含量的无损伤、现场检测技术；[2]应用C30-HPLC-PDA-ELSD技术实现了对番茄红素几何异构体的分离，测定了不同顺式异构体的吸光系数，建立了天然番茄红素几何异构体组成的检测方法，为区分和鉴定人工合成品和天然产物奠定了基础；[3]证实了秋橄榄果实中的番茄红素资源具有工业开发价值，并建立了超临界CO_2萃取的实验室方法；[4]探索了提高番茄红素超临界流体萃取效率的方法，证明了物料前处理对提高萃取效率起了极其重要的作用；[5]观察了番茄红素在大鼠体内几何异构体组成的变化情况，发现了大量顺式异构体的存在，并证实了几何异构体组成在血清中发生了显著变化；[6]测定了天然番茄红素在大鼠体内的吸收率和在大鼠血清中的动态积累规律；[7]比较了不同番茄红素几何异构体淬灭单线态氧的能力；[8]检测了天然番茄红素是否具有调节血脂作用和抗低密度脂蛋白氧化的功能；[9]首次发现大鼠前列腺内存在番茄红素C31降解片段，并证实其仍具有淬灭单线态氧的能力。

Scripps Institute of the United States and his马克耶格尔Professor University of Virginia, the University of Utah colleagues used X-ray crystal photography, the first detailed description of the composition of the HIV virus capsid protein precursors of the hexagonal molecular structure of high-definition, they said hexagonal protein precursors such as CA VI protein oligomers.

美国斯克里普斯研究所的马克。耶格尔教授和他在弗吉尼亚大学、犹他大学的同事一起，采用X光晶体照相术，第一次详细描述了构成HIV病毒衣壳的六边形蛋白体的高清分子结构，他们称这种六边形蛋白体为CA蛋白六聚物。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。