蛋白光

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组，以体外直肠不全结扎法造模，以动态浊度法检测各组大鼠血浆内毒素水平，放射免疫分析法检测各组血清TNF-α含量；②大鼠处死后，取左肺进行支气管肺泡灌洗，以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平；③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数，计数兰染的细胞数，求出死亡百分率；在光镜下计数BALF沉渣200个细胞，计算中性粒细胞所占百分比。

As they report in the Public Library of Science, HenrikMouritsen of the University of Oldenburg, in Germany, and his colleagues, thinkthat proteins called cryptochromes are involved.

德国奥尔登堡大学的亨利克·莫里特森及其同事们在《公共科学图书馆》杂志上发表的报告认为，这与一种名叫隐色素（亦称蓝光受体， cryptochrome ）的蛋白有关。

Rice (Oryza sativa L.) is not only one of the most important food crops in the world, but also a model plant for the study of molecular developmental biology in cereals.

很多研究已表明，生长素的运输蛋白在植物的胚后发育过程及植物的光形态建成和组织分化方面起着非常重要的作用。

In the study, we established the animal model of diabetic ED successfully. Modern medical methods such as routine lightscopy, pathology, transmission electroscopy, scanning electroscopy, fluorospectrophotometer and radio-immunity were employed. From the aspects of pathomorphism, cellular supermicrostucture and neurocrine, we observed the mixture's effect on status of the whole animal model and penial erection, the fast plasma glucose, protein non-enzyme gycosylation, nitric oxide synthase , testosterone. In contrast with control groups, the mixture could apparently slow down the corpus cavernosum penial, vascular, neural pathologic progression of the diabetic rats and had a better result than diabetic pill, invigorating yang and restoring normal capsule and amino guanidine.

通过实验研究，成功建立了糖尿病性阴茎勃起功能障碍动物模型，利用常规光镜病理、透射电镜、扫描电镜、荧光分光光度计、放射免疫法等现代医学研究方法，从病理形态学、细胞超微结构、神经内分泌学等方面，观察了降糖起萎合剂对模型动物整体状态和阴茎勃起情况、空腹血糖水平、蛋白非酶糖基化、一氧化氮合酶、睾酮等实验指标的影响，与对照组比较提示降糖起萎合剂能明显延缓大鼠基于DM基础上的阴茎海绵体及血管、神经病变的病理进程，其疗效优于消渴丸、壮阳复春灵胶囊及氨基胍。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

It is possible that the 60KD protein is an efficient marker to determine whether a rice line has the steady photoperiodic sensitive male sterility.

60KD蛋白可能是判断一个水稻材料是否具有稳定光敏不育特性的有效指标。

Phytochrome A proteinaceous pigment found in low concentrations in most plant organs, particularly meristems and darkgrown seedlings.

光敏色素：在大多数植物器官，特别是在分生组织和黄花苗中低浓度存在的蛋白色素。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The glycyrrhizic acid antigen is prepared through the process including following steps: dissolving glycyrrhizic acid in N, N-dimethyl formamide, dioxane or dimethyl sulfoxide, heliophobically adding N-hydroxy succinimide, reacting with dicyclohexylcarbodiimide at 0-10 deg.c for 2-5 hr; and reacting with carrier protein at normal temperature for 4-6 hr to obtain glycyrrhizic acid antigen.

本发明所提供的甘草酸抗体，是用甘草酸抗原免疫动物，然后从被免疫动物的血清中分离得到的；所述甘草酸抗原按如下过程制备：先将甘草酸溶解在N，N-二甲基甲酰胺、二氧六环或二甲基亚砜中，避光加入N-羟基琥珀酰亚胺，然后在0-10℃条件下与二环己基碳二亚胺反应2-5小时；接着与载体蛋白常温反应4-6小时，得到甘草酸抗原。

The P-Phycoerythrin is a less toxity and prosperous photosensitizer possibly applied to the photodynamic therapy of cancer.

藻红蛋白是一种毒性较小，很有前景的光敏剂，可望应用于肿瘤光动力治疗。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。