蛋白休克

ABSTRACT Antitumor Activity of Dendritic Cells Activated with gP96 Peptide Complex Department of Thoracic Surgery, Peking University People's Hospital Objectives: To investigate the expression level of gP96 in human lung cancer samples; purify gP96-polypeptide from human and mouse lung adenocarcinoma cell lines; prepare tumor vaccine by in vitro gP96-polypeptide stimulation of monocyte-derived dendritic cells and test its efficiency both in vitro and in mice models.

研究人肺癌组织热休克蛋白gP96—多肽复合物的表达水平；肺癌细胞株来源的肿瘤组织gP96—多肽复合物的提取和鉴定；外周血来源树突状细胞培养和鉴定：将gP96—多肽复合物和DC体外共同孵育制备疫苗，在体外激活T淋巴细胞的能力及在小鼠体内的抑制肿瘤生长作用的研究。

Objective To review the advancement of heat shock protein 70 （HSP70） vaccine in alimentary canal cancer.

目的综述热休克蛋白70（heat shock protein70，HSP70）疫苗在消化道肿瘤中的研究进展。

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活，ICAM-1表达升高，与被激活的PMN表面表达的LFA-1相结合，&PMN-EC&相互作用加剧，一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍；另一方面粘附的PMN过度吞噬或激活，当白细胞吞噬的颗粒不能被封闭隔离，连同细胞内的酶被释放出来，其中的PMN-elastase能够降解细胞基质中各种成分，水解多种蛋白，加重胰腺的毛细血管内皮细胞和腺泡的损伤；释放的溶酶体酶使组织蛋白水解，产生的不饱和脂肪酸引发脂质过氧化方应，破坏细胞膜的结构和功能；释放的炎性细胞因子，激发其他的免疫细胞的功能，导致进一步的组织损伤和坏死，加重SAP的病理损伤，最终导致休克、脓毒血症及多器官功能障碍等严重后果。

Small heat shock proteins are a particularly important group of chaperones. They prevent the clumping of proteins under stress conditions.

小的热休克蛋白是分子伴侣中非常重要的一个群体，它们阻止蛋白在应力状态下的聚合。

DATA SYNTHESIS: HSP70 is one of the most conservative proteins during biological evolution.

资料综合：热休克蛋白70是生物进化过程中最为保守的一族蛋白。

Recent study indicates that heat shock protein, a protective protein, could also be used as the marker of overtraining.

最新研究显示，热休克蛋白作为一种应激保护性蛋白，也是监控过度训练的有效指标。

It has been suggested that HSP function as molecular chaperons, which direct the proper folding of the protein, as well proper assembly of the protein complex.

热休克蛋白的功能是作为分子监护子，导引蛋白质正确折叠和适当组装蛋白复合体。

Objective To observe the relationship between zonula occludens protein subunit 1(ZO-1)and the regulatory effectof vasodilator stimulated phosphoproteinon the intestinal barrier function inrats ofhemorrhagic shock.

目的 观察血管扩张刺激磷蛋白(vasodilator stimulated phosphoprotein， VASP)调节失血性休克大鼠小肠屏障功能与闭锁连接蛋白1(ZO-1)的关系。

Heat shock protein 72 may contribute to cell apoptosis by upregulating P53 expression.

热休克蛋白72可能通过上调p53蛋白的表达促进细胞凋亡。

There is an increasing need to better understand the fate of domoic acid, in aquatic ecosystem. In this report, partial cDNA of hepatic cytochrome P-450 1A (CYP1A), giutathione S-transferase alpha, glutathione S-transferase rho, heat shock protein 70 (HSP70), Na~+/K~+-ATPase alpha 1(ATP 1A1) and beta-actin gene from rabbitfish were obtained by PCR using degenerate primers, which respectively were 900 bp, 582 bp, 615 bp, 660 bp, 749 bp and 554 bp in length.

本研究成功克隆了黄斑篮子鱼肝脏第Ⅰ时相去毒酶细胞色素P450 1A，第Ⅱ时相去毒酶谷胱甘肽S-转移酶alpha和谷胱甘肽S-转移酶rho(GS7R)，热休克蛋白70(HSP70)，钠钾ATP酶alpha 1(ATP1A1)和β-肌动蛋白基因的核心序列，它们分别长900 bp，582 bp，615 bp，660 bp，749 bp和554 bp。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐