蛋白休克

The complications in the early stage, related parameters, and the incidence rate of infection and mortality in the later stage were evaluated respectively. Results: The incidence rates of renal dysfunction, shock, cardiovascular failure and gastrointestinal hemorrhage, the score of acute physiology and chronic health evaluation Ⅱ and the frequencies of pulse and breath in the severe hypoalbuminemia group were all higher than those in the mild hypoalbuminemia group (P＜0.05 or P＜0.01). The differences of incidence rate of hepatic failure and the scores of Ranson and Balthazar CT between these two groups had no statistical significance (P＞0.05). The incidence rate of infection and the mortality in the severe hypoalbuminemia group were higher than those in the mild hypoalbuminemia group (P＜0.01) in the later stage of SAP.

结果：重度低白蛋白血症组肾功能衰竭、休克、心衰及消化道出血的发生率高于轻度低白蛋白血症组（P＜0.01，肝功能衰竭发生率两组比较无统计学差异；Ranson评分及Balthazar CT评分两组间比较，差异无统计学意义（P＞0.05）；重度低白蛋白血症组的急性生理和慢性健康评价指标Ⅱ（acute physiology and chronic health evaluationⅡ， APACHEⅡ）评分、脉搏和呼吸频率均明显高于轻度低白蛋白血症组（P＜0.05或P＜0.01；重度低白蛋白血症组后期感染发生率及病死率均高于轻度低白蛋白血症组

RESULTS: Image analysis of two-dimensional map revealed 72 differentially expressed proteins between MHCC97-H and MHCC97-L cells, and 9 protein peaks (including M2 pyruvate kinase,α-subunit of ATP synthase, heat shock protein 60, Toll-like receptor 9, flavoprotein oxygenase, pro-calreticulin, manganese superoxide dismutase, nm23-H1, and G-protein-coupled receptor kinase 5) were further identified by tryptic digestion, peptide mass fingerprinting and mass spectrometry.

结果： 2D图谱显示共有72个差异蛋白条带，共鉴定出了9个差异蛋白，分别为M2型丙酮酸激酶、ATP合成酶α亚单位、热休克蛋白60、Toll样受体9、含黄素单加氧酶、钙网硬蛋白前体、锰超氧化物岐化酶、nm23-H1、G-蛋白偶连受体激酶5；其中4个蛋白在高转移细胞株MHCC97-H中表达升高， 5个蛋白在低转移细胞株MHCC97-L表达升高。

Objective: To study the expression of heat shock protein 70(HSP70) following transient ischemia in the gerbil.

目的：研究热休克预处理对脑缺血再灌后热休克蛋白70(HSP70)的表达影响及对缺血神经细胞损伤的保护作用。

An early event in a cellular heat shock response is the transmittance of stress signal from cell surface into the interior and the...

在细胞应答热休克过程中最早期的事件是将胁迫信号从细胞表面传至细胞内，从而诱导热休克蛋白的合成使细胞产生对热的耐受性。

Twenty-seven differential proteins were tested by MALDI-TOF-MS, and 16 were identified. Of the 16 proteins, 13 were up-regulated in patients with HCC, including heat-shock protein 27, member RAS oncogene family (RAB14), actin, alpha 1 antitrypsin, RNA-binding protein regulatory subunit and so on. By contrast, the levels of proteins such as PDX6, HSPA8 and MnSOD were significantly reduced in HCC patients.

对27个差异蛋白质点进行了质谱分析，鉴定出16个蛋白质，在HBV相关性HCC患者PBMC中表达明显增强的有热休克蛋白质27、ras癌基因家族(RAB14)、肌动蛋白、α1-抗胰蛋白及RNA结合蛋白调节亚基等13个蛋白点；而PDX6、HSPA8及锰超氧化物歧化酶在HCC患者PBMC中低表达。

RESULTS: A well-resolved and reproducible 2-DE pattern of PBMC was obtained from patients with HBV associated HCC or LC. For HCC, the mean number of protein spots was 1186±43, with an average matching rate of 91.6%, and for LC, the mean number of protein spots was 1013±41, with an average matching rate of 90.2%. Twenty-seven differential proteins were tested by MALDI-TOFMS, and 16 were identified. Of the 16 proteins, 13 were up-regulated in patients with HCC, including heat-shock protein 27, member RAS oncogene family (RAB14), actin, alpha 1 antitrypsin, RNAbinding protein regulatory subunit and so on. By contrast, the levels of proteins such as PDX6, HSPA8 and MnSOD were significantly reduced in HCC patients.

结果：得到了分辨率较高、重复性较好的HBV相关性HCC及LC患者PBMC双向凝胶电泳图谱，HBV相关性HCC及LC患者PBMC凝胶的平均蛋白质点数分别为1186±43及1013±41；组内的平均匹配率分别为91.6%，90.20%对27个差异蛋白质点进行了质谱分析，鉴定出16个蛋白质，在HBV相关性HCC患者PBMC中表达明显增强的有热休克蛋白质27、ras癌基因家族(RAB14)、肌动蛋白、α1-抗胰蛋白及RNA结合蛋白调节亚基等13个蛋白点；而PDX6、HSPA8及锰超氧化物歧化酶在HCC患者PBMC中低表达。

Sequences analysis indicated 26 unique cDNA sequences that encoded putative proteins showing similarities to HL35 antigen U, hlim3, heat shock protein, NADH dehydrogenase subunit 1 and calreticulin of H. longicornis, disulfide isomerase of Ambiyomma variegatum and Ixodes scapularis.

结果表明：在长角血蜱雌蜱唾液腺cDNA表达文库中获得26个长角血蜱免疫相关cDNA序列，其编码蛋白与长角血蜱HL35、黏附素hlim3假定蛋白、热休克蛋白、NADH脱氢酶、钙网蛋白以及杂色花蜱和肩突硬蜱的二硫异构酶等具有同源性。

Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23 A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells.

结果：筛选出21个差异蛋白质点，成功鉴定出5种差异蛋白质，其中在LoVo细胞株中表达上调的蛋白有埃兹蛋白、转录延长因子A (transcription elongation factor A)和甘氨酸A/C，在SW480细胞株中表达上调的蛋白有NM23蛋白[non-metastatic cell 1 protein (NM23A) expressed in isoform a]和（heat shockprotein 8，热休克蛋白8）。

To determine whether F3, similar to other large DNA iruses, encoded proteins that lock or delay apoptosis, mock- and F3-infected FHM cells were osmotically shocked and assayed for DNA fragmentation 3 hours later. NA fragmentation was clearly seen whether or not shocked cells were preiously infected with F3, indicating that infection with F3 did not block apoptosis induced by osmotic shock in FHM cells.

为了证明F3是否象其大DNA病毒一样编码能阻止或延迟凋亡的蛋白，在模拟感染和正常感染的FHM细胞被渗透压休克后3小时检测DNA片段化；结果显示无论是否休克F3感染都能诱导FHM细胞的DNA片段化，渗透压休克并不能阻止细胞凋亡。

The results indicated that the histone deacetylase inhibitors had effects on the morphology of the polytene chromosomes.

2，利用RT-PCR技术，对去乙酰化酶抑制剂处理后的果蝇的热休克基因的表达水平进行检测，结果表明经组蛋白去乙酰化酶抑制剂处理后的果蝇幼虫，其热休克基因的表达高于基础水平，也就是说去乙酰化酶抑制剂诱导了热休克基因的表达。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失