蛋白

It is a member of a large family of proteins characterized by two conserved domains and a set of 16 conserved cysteine residues.

它是一个庞大的蛋白家族的一员，这个蛋白家族有两个保守区域和16个保守的半胱氨酸残基。

Metallothioneinis a group of conjugated proteins which are rich in cysteine with low molecular weight.

金属硫蛋白是一类富含半胱氨酸的低分子量结合蛋白。

The cDNA has 752bp and encodes a protein of 216 amino acids. Thenascent Src l contained a signal peptide of 19 amino acids, a propart of 19 amino acidsand a 178-amino-acid mature Src l. The characteristic amphiphilic α-helix of cytolysin islocated at the N-terminus of the mature Src l.

根据文库的EST序列设计引物，从玫瑰红绿海葵克隆到一全长cDNA序列，命名为Src I，利用生物信息学方法对其进行了分析，结果表明，该cDNA序列编码一个216个氨基酸的海葵细胞毒素前体蛋白，它包括19个氨基酸的信号肽、19个氨基酸的propart motif和178个氨基酸的成熟蛋白。

In contrast, cancer cells lacking functional P53 would be expected to be sensitive to viral replication and subsequent cytolysis. However P53 defections wildly exist in most of human cancer cell, deletion of E1B-55kD gene can make the adenovirus target at p53 mutation tumor cell.

以往的研究表明，大部分人类肿瘤中均存在p53基因突变，因此E1B55kD蛋白缺失的重组病毒只能在P53功能异常的肿瘤细胞中复制，而在正常细胞中该重组病毒由于缺乏对抗P53蛋白的病毒产物，不能有效复制，因此具有肿瘤靶向治疗的特点。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

The fragment encoding the polypeptide of wheat MLO protein C terminus located in cytoplast was cloned into the vector pET-30a and expressed in the strain BL21 of E coli.

本研究将编码小麦MLO蛋白羧基端约130个氨基酸多肽的基因片断克隆到表达载体pET-30a中，在大肠杆菌中表达了该多肽的融合蛋白。

Pombe Protein kinase cascade regulates the response of extracellular stimulation in cytoplast and cell nuclei. The MAPK signal cascade is an ancestral and conservative protein kinase in eukaryote.

MAPK(mitogen-activated protein kinase，丝裂原激活的蛋白激酶)级联是在单细胞和多细胞真核生物中发现的古老而保守的蛋白激酶级联。

Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells. 2 CldAdo blocked the cell growth at the phase of S and G2/M, while 2 ClAdo at the phase of G0/G1. 2 CldAdo mainly induced cell necrosis, while 2 ClAdo induced both cell necrosis and apoptosis. Down regulated Bcl 2 expression and up regulated p53 and Bax expression were associated with 2 ClAdo, while 2 CldAdo down regulated Bcl 2 expression and up regulated mildly p53 expression without any effect on Bax expression.

结果 2 ClAdo、2 CldAdo对所研究的细胞均有程度不等的毒性；2' dAdo对各细胞生长、增殖均无明显抑制（c=100 μmol/L即30 μg/mL内）；2 CldAdo明显阻断细胞于S、G2/M期，2 ClAdo阻断细胞于G0/G1期；2 CldAdo 致细胞坏死为主，2 ClAdo 既致细胞坏死又诱导凋亡；2 ClAdo 下调Bcl 2，上调P53、Bax蛋白表达；2 CldAdo小幅上调P53、小幅下调Bcl 2，不影响Bax蛋白表达。

Further bioinformatical analysis indicated that the three Y-box genes of lamprey, i.e., Lyb1, Lyb2, and Lyb3, encoded 331, 181, and 171 amino acids, respectively. The three amino acid sequences were homologically aligned with those from Ciona intestinalis, Danio rerio, Oryzias latipes, Takifugu rubripes, and Mus musculus by DNAMAN software.

采用DNAMAN软件，将七鳃鳗的3个Y-box蛋白氨基酸序列与海鞘、斑马鱼、清鳉、河豚鱼和小鼠Y-box蛋白进行同源性分析，结果显示他们共有一个保守的冷休克结构域，其中包含两个RNA结合基序RNP-1和RNP-2，表明获得的Lyb基因属于Y-box 基因家族成员。

Sequence analysis reveals the identity rate of β-actin cDNA nucleotide sequence between the rice field eel and Tilapiamossambica, Salmo salar, Oryzias latipes, Oncorhynchus mykiss, Danio rerio, Carassius auratus, Physalaemus pustulosus, Bos taurus, Gall us gallus, Mus musculus, Hoino sapiens are 68%~95%; and the similarity of 3-actin amino acid sequence is 97%~100%.

将所得序列与各科鱼、蛙、鸡、牛、鼠、人等的β-肌动蛋白基因序列同源性分析显示，核苷酸序列具有68%~95%的相似性，氨基酸序列具有97%~100%相似性，显示该基因在生物进化过程中的保守；系统发育分析表明黄鳝β-肌动蛋白基因与罗非鱼的亲缘关系最近。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失