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Methods Mimicry and speculation on possible displaced amino acids in proper positions of peptide increased the biological effects of TNFR blocking peptide by compote modeling. The original peptide and its mutant were synthesized. The competitively inhibitory effects on green fluorescence protein-tumor necrosis factor fusion protein with TNFR were compared between original peptide and its mutant (receptor competitively inhibitory binding assays). The inhibitory effects on cytotoxicity were compared between original peptide and its mutant.

通过计算机模建,模拟并推测提高TNFR封闭肽生物学效应需置换的位点和氨基酸;合成原封闭肽和突变肽;受体竞争抑制结合实验比较原肽与突变肽对绿色荧光蛋白-肿瘤坏死因子融合蛋白结合TNFR的竞争抑制效应;细胞毒作用抑制实验比较原肽与突变肽对TNFR的封闭效应。

In this thesis, we studied the effects of nuclear actin on gene transcription regulation, with a special focus on the relations between the nuclear actin and RNA polymerase II in concanavalin A-stimulated lymphocytes and HeLa cells.

本文以ConA激活的淋巴细胞和HeLa细胞为实验材料,从不同角度研究了细胞核肌动蛋白与RNA聚合酶Ⅱ相互作用,并对细胞核肌动蛋白参与RNA聚合酶Ⅱ的作用机制进行了探讨。

The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia.

实验前后用睫状肌麻痹下验光、A超动态观察豚鼠眼屈光度和眼轴长度,并取后极部巩膜组织,用电镜、终末脱氧核糖核苷酸转移酶介导原位缺口末端标记法和流式细胞术检测巩膜的凋亡细胞,应用免疫组织化学染色和蛋白印迹(Western-blot)法检测bFGF和bcl-2蛋白

They were characterized by decreased fluidity in the deeper part of membrane lipid,configurational changes and motion restriction in membrane proteins.

严重烧伤后早期即出现心肌细胞膜的损伤,表现为膜脂质深层流动性下降,膜蛋白构象改变,膜蛋白运动受限。

The aluminium oxyhydrogen adjuvant could increase the IgG titer to Pncps caused by the Pncps-protein conjugant, very notably increase the IgG titer to protein and to conjugant itself caused by conjugant.

氢氧化铝佐剂能增加多糖-蛋白结合物诱生的抗多糖IgG抗体滴度,极其显著地增加抗蛋白及抗结合物自身的IgG抗体滴度。

Among them, the composition of membrane proteins, the protein in connective tissue component.

其中,蛋白参与细胞膜的组成成分,蛋白参与结缔组织的组成。

Among them, the SCAN domain is a highly conserver motif that is vertebrate-specific.

众多含有SCAN结构域的C2H2型锌指蛋白组成了一个C2H2型锌指蛋白的亚家族。

Fibronectin and laminin were responsible for this contractile action.

纤维连接蛋白和层粘连蛋白参与了膜收缩。

Endothelial cell form and contractility are regulated by cytoskeleton proteins,such as actin and myosin.

内皮细胞形态变化和收缩性的改变主要受细胞骨架蛋白如肌动蛋白和肌球蛋白的影响。

All the research work has laid a solid foundation for the study on Proteomics of Conus venom duct.

通过以上的研究为芋螺毒管蛋白蛋白组学的深入研究打下良好的基础。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。