蛋白

The expression level of integrin β1 on the surface of K562 cells was evaluated by flow cytometry; the growth of K562 cells treated with phycocyanin was measured by MTT assay; the expression level of FAK mRNA was analyzed by relatively quantitative RT-PCR after four-day culture of K562 cells with phycocyanin of 40 μg/ml,80 μg/ml and 160 μg/ml,respectively.

本 研究观察不同浓度藻蓝蛋白对K562细胞增殖的影响，并检测藻蓝蛋白处理后细胞表面整合蛋白β1表达和胞内黏着斑激酶基因表达的变化，探讨整合蛋白β1在藻蓝蛋白抑制K562细胞增殖中的可能作用机制。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The baculovirus expression system, utilizing the strong polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus , has become an important tool for protein production in the field of molecular biology.

由于核型多角体杆状病毒的多角体蛋白不是病毒复制必需的蛋白，利用转录多角体蛋白基因的强启动子可以高效表达外源蛋白，包括原核和真核基因编码的蛋白。

Plant there exists large amount of cell wall egg white in cell wall middle, if structure egg white , zymoprotein etc., structure egg white having mainly expanding egg white , being rich in the glycine proteide and so on studying at present, brings the important effect into play in plant cell wall growth and structural adjustment.

植物细胞壁中存在大量的细胞壁蛋白，如结构蛋白、酶蛋白等，目前研究的结构蛋白主要有伸展蛋白、富含甘氨酸的蛋白质等，在植物细胞壁的生长和结构调整中发挥重要作用。

IntroductionProtein kinase B is a serine /threonine protein kinase with homology to protein kinase A and C , so it is also called RAG.

前言 蛋白激酶B是丝氨酸／苏氨酸蛋白激酶，它与蛋白激酶A和蛋白激酶C具有相似的氨基酸序列，所以又叫RAC蛋白激酶。

Its main composition and nutritional value,as well as new methods and technology of deep processing based on CGM at home and abroad,are introduced in this article,including development and production of zein isolated protein and corn bioactive peptides with many special health functions.

文中介绍了玉米蛋白的主要组成成分、营养价值，重点综述了国内外玉米蛋白深加工的新方法和新工艺，包括加工生产醇溶蛋白、玉米分离蛋白以及具有多种保健功能的玉米蛋白肽等。

The persent of each component protein can be measured :total protein 52.72 per, albumin 14.36 per, globin 9.30per, titanium 12.87 per , alcoholy protein 2.29per, alkalize protein 2.62per, hawless protein 6.08per.

结果发现：脱脂蝇蛆粉的总蛋白质含量为52.72%，清蛋白14.36%，球蛋白9.30%，小分子蛋白12.87%，醇溶蛋白2.29%，碱溶蛋白2.62%，难溶蛋白6.08%。

The p53 gene structural abnormality and mdm2 protein overexpression might be two factors causing p53 protein overexpresion in NPC. The function of p53 proteins produced by various structural anormality of p53 gene might be different in activating MDM2. The overexpression of p53 or mdm2 protein, particularly both cooperation, might play an important role during NPC genesis.Key words: Nasopharyngeal neoplasms; p53 gene; p53 protein; mdm2 protein

p53基因结构改变和mdm2蛋白过度表达是影响NPCp53蛋白过度表达的两个因素；不同类型p53基因结构改变所产生的p53蛋白激活MDM2的功能并不相同；p53和mdm2蛋白过度表达，尤其两蛋白协同作用，可能在NPC发生中起重要作用。

We isolate the LCA binding glycosylated proteins from anosia human liver cell extraction and liver cancer cell extraction by LCA coupled epoxy magnetic particles, and the glycosylated fractions were separated by 2D gels which were analyzed by 2D ImageMaster to established disparity expression spectra between Chang Liver and SMMC-7721 firstly.

利用该方法对健康人肝脏细胞Chang Liver总蛋白、肝癌细胞SMMC-7721总蛋白及健康人血清进行了糖蛋白的分离纯化，结合2D电泳技术及其图像分析，建立了健康人肝脏细胞ChangLiver和肝癌细胞SMMS-7721 LCA结合糖蛋白的差异表达谱，分别得到蛋白质点148±5和209±7个，匹配点数为127，匹配率为85.36%。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失